Results 81 to 90 of about 601,747 (289)

Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis

BMC Research Notes, 2019
Objective Planarians including Dugesia ryukyuensis (Dr) have strong regenerative abilities that require enhanced DNA replication. Knockdown of the DUT gene in Dr, which encodes deoxyuridine 5′-triphosphate pyrophosphatase (dUTPase), promotes DNA ...
Md. Shahanoor Alam, Hideaki Moriyama, Midori Matsumoto   +2 more
doaj   +1 more source

Orotic Aciduria [PDF]

, 2018
Orotic acid is an intermediate found in the pathway for pyrimidine synthesis. The mitochondrial enzyme dihydroorotate dehydrogenase (DHODH) catalyzes the production of orotic acid by the conversion of the compound dihydroorotate to orotic acid.
Fonteh, Aliah L
core   +1 more source

Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons [PDF]

, 2015
We describe here a detailed protocol for the synthesis of ribonucleotides specifically deuterated at each ribose carbon atom. We synthesized 20 specifically deuterated ribonucleotides: ATP, CTP, GTP, and UTP, each of which contained one of five ...
Azad, Robert, Ingle, Shakti, Tullius, Tom   +2 more
core   +3 more sources

Phase Separation of Nucleic Acids: Mechanisms, Properties, and Applications

Angewandte Chemie International Edition, EarlyView.
Recent discoveries have shown that single‐stranded long‐chain nucleic acids can undergo temperature‐induced phase separation, enabling formation of micrometer‐sized condensates. This Minireview discusses the current mechanistic understanding of this phenomenon, highlights strategies for controlling the physical and chemical properties of these ...
Weixiang Chen, Johann Fritzen, Andreas Walther   +2 more
wiley   +1 more source

NMR Metabolomic Analysis of Skeletal Muscle, Heart, and Liver of Hatchling Loggerhead Sea Turtles (Caretta caretta) Experimentally Exposed to Crude Oil and/or Corexit

Metabolites, 2019
We used nuclear magnetic spectroscopy (NMR) to evaluate the metabolic impacts of crude oil, Corexit 5900A, a dispersant, and a crude oil Corexit 5900A mixture exposure on skeletal muscle, heart, and liver physiology of hatchling loggerhead sea turtles ...
Stasia A. Bembenek-Bailey, Jennifer N. Niemuth, Patricia D. McClellan-Green, Matthew H. Godfrey, Craig A. Harms, Hanna Gracz, Michael K. Stoskopf   +6 more
doaj   +1 more source

Density Functional Theory for the Photoionization Dynamics of Uracil

, 2007
Photoionization dynamics of the RNA base Uracil is studied in the framework of Density Functional Theory (DFT). The photoionization calculations take advantage of a newly developed parallel version of a multicentric approach to the calculation of the ...
Altmann S. L., D. Toffoli, F. A. Gianturco, Massey H. S. W., P. Decleva, Parr R. G., R. R. Lucchese, Slater J. C., Taylor J. R.   +8 more
core   +1 more source

Synthesis of 1,4-Disubstituted Mono and Bis-triazolocarbo-acyclonucleoside Analogues of 9-(4-Hydroxybutyl)guanine by Cu(I)-Catalyzed Click Azide-Alkyne Cycloaddition [PDF]

, 2011
A series of novel mono-1,2,3-triazole and bis-1,2,3-triazole acyclonucleoside analogues of 9-(4-hydroxybutyl)guanine was prepared via copper(I)-catalyzed 1,3-dipolar cycloaddition of N-9 propargylpurine, N-1-propargylpyrimidines/as-triazine with the ...
Engels, Joachim W., Krim, Jamal, Taourirte, Mohamed   +2 more
core   +2 more sources

Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme‐derived peptide tag

Biotechnology Progress, EarlyView.
Fusion of a 27‐amino‐acid peptide tag derived from a glycolytic enzyme enabled artificial assembly formation of the two violacein biosynthetic enzymes, VioE and VioC. This assembly resulted in enhanced biosynthesis of deoxyviolacein. Abstract In microbial production, regulating endogenous and exogenous synthetic pathways is essential.
Rioko Ito, Sayoko Ito‐Harashima, Naoko Segawa, Natsuko Miura, Michihiko Kataoka   +4 more
wiley   +1 more source

Synthetic RNA modules for fine-tuning gene expression levels in yeast by modulating RNase III activity [PDF]

, 2011
The design of synthetic gene networks requires an extensive genetic toolbox to control the activities and levels of protein components to achieve desired cellular functions.
Alper, Alper, Alper, Anderson, Andrew H. Babiskin, Babiskin, Babiskin, Basu, Beisel, Callura, Caponigro, Carrier, Chanfreau, Chanfreau, Chao, Christina D. Smolke, Danin-Kreiselman, Elela, Ellis, Elowitz, Gardner, Ge, Gietz, Hawkins, Hawkins, Isaacs, Jensen, Jin, Jones, Keiler, Lamontagne, Lamontagne, Lamontagne, Lamontagne, Lamontagne, Lamontagne, Larose, Lautz, Lavoie, Lee, Louis, Lutz, Mateus, Misra, Nevoigt, Ng, Nguyen, Ostergaard, Paradise, Pfleger, Ro, Salis, Sambrook, Szczebara, Vasudevan, Veen, Win, Wu, Wu, Zhou, Zhou, Zhu, Zhu   +62 more
core   +3 more sources

Recent Advances in Chemical Probing Strategies for RNA Structure Determination in Vivo

Chemistry – A European Journal, EarlyView.
This Review illustrates how structural probes combined with advanced sequencing technologies are revolutionizing our ability to map RNA structures and interactions within living cells. It highlights the development of innovative strategies tailored to probe RNA structures in particular biological contexts.
Maryana Yarshova, Jieyu Zhao, Chun Kit Kwok   +2 more
wiley   +1 more source