Cell-Free Multi-Enzyme Synthesis and Purification of Uridine Diphosphate Galactose. [PDF]
Chembiochem, 2022 AbstractHigh costs and low availability of UDP‐galactose hampers the enzymatic synthesis of valuable oligosaccharides such as human milk oligosaccharides. Here, we report the development of a platform for the scalable, biocatalytic synthesis and purification of UDP‐galactose.
Mahour R +8 more
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Journal of Lipid Research, 1976 An enzyme that transfers galactose from UDP-Gal to ganglioside GM2 (Tay-Sachs ganglioside) was concentrated 50 times in Golgi apparatus from rat liver relative to total homogenates.
F E Wilkinson, D J Morré, T W Keenan
doaj +2 more sources
The enzymatic transformation of uridine diphosphate glucose into a galactose derivative [PDF]
Archives of Biochemistry and Biophysics, 1951 Abstract Treatment of uridine diphosphate glucose (UDPG) with an enzyme of S. fragilis was found to produce about 25% of a galactose-containing compound. This compound is precipitated with mercuric ions like UDPG, and its migration in chromatography in acid-ethanol is similar.
Luis F. Leloir
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Frog brain uridine diphosphate galactose–N-acetylgalactosaminyl-N-acetylneuraminylgalactosylglucosylceramide galactosyltransferase [PDF]
Biochemical Journal, 1970 1. The enzyme that catalyses the transfer of galactose from UDP-galactose to N-acetylgalactosaminyl-(1→4)-N-acetylneuraminyl-(2→3)-galactosyl-(1→4)-glucosylceramide (GM2) was found mainly in the heavy- and light-microsomal fractions of the adult frog brain. 2.
Morris C.M. Yip, Joel A. Dain
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Generalised uridine diphosphate galactose-4-epimerase deficiency [PDF]
Archives of Disease in Childhood, 1999 The generalised form of epimerase deficiency galactosaemia has been described in only two children from unrelated families. Their progress is reported and three other affected children from these families are described. The initial presentation was similar to classic galactosaemia.
J H Walter +6 more
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Developmental aspects of uridine diphosphate galactose 4-epimerase in rat intestine [PDF]
Biochemical Journal, 1971 Kinetic and developmental characteristics of rat intestinal UDP-galactose 4-epimerase activity have been examined. The enzyme in the adult rat had a Vmax. value 2–3 times higher than that of the newborn animal, but the Km values for the enzyme in the newborn and adult rat were the same (0.17mm). No differences in epimerase activity were found along the
Arthur Weinstein +2 more
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Lactogenesis in the rat. Metabolism of uridine diphosphate galactose by mammary gland [PDF]
Biochemical Journal, 1968 1. Lactose synthetase activity in the rat mammary gland increases during the last day of pregnancy from an essentially zero value. There is a parallel increase of tissue lactose and of glucose 6-phosphate dehydrogenase activity. 2. Mammary-gland homogenates prepared both before and after parturition hydrolyse the lactose precursors glucose 6-phosphate,
Nicholas J. Kuhn
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Uridine Diphosphate-4-Keto-Glucose, an Intermediate in the Uridine Diphosphate-Galactose-4-Epimerase Reaction [PDF]
Proceedings of the National Academy of Sciences, 1971 When UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli is incubated with UDP-galactose, then reduced with NaB 3 H 4 , label is found in UDP-glucose and UDP-galactose.
Utpalendu S. Maitra, Helmut Ankel
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Enzymic synthesis of cerebroside from ceramide and uridine diphosphate galactose [PDF]
Biochemical Journal, 1969 Yozo Fujino, Masahiro Nakano
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Human Hepatic Uridine Diphosphate Galactose Pyrophosphorylase
Journal of Biological Chemistry, 1969 Hajnalka Ábrahám, R. Rodney Howell
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