Results 11 to 20 of about 3,929 (139)

Osmolality‐Independent Impact of Sodium on Glycosylation of an Fc‐Fusion Protein and the Hexosamine Biosynthesis Pathway in a Chinese Hamster Ovary Cell Line [PDF]

Biotechnology Journal, Volume 21, Issue 6, June 2026.
Graphical Abstract and Lay Summary Sodium and potassium supplementation in CHO batch culture elevates intracellular UDP‐HexNAc concentrations through activation of the hexosamine biosynthesis pathway (HBP), independently of osmolality. Increased nucleotide sugar availability enhances Fc‐fusion protein glycosylation and reduces the abundance of low ...
Jason Reiniger, Adrian Pöttner, Lara Rosenberger, Aline Zimmer   +3 more
wiley   +2 more sources

Multienzyme Platform for the Synthesis of UDP Sugars and Human Milk Oligosaccharides [PDF]

ChemBioChem, Volume 27, Issue 8, April 2026.
Engineered Escherichia coli produces six enzymes (UMPK, PPK3, GALK, NAHK, GALU, PPA) that are purified and used in a cell‐free cascade to synthesize UDP‐N‐acetylglucosamine (UDP‐GlcNAc) and UDP‐galactose (UDP‐Gal) from inexpensive substrates. The cascades can be coupled with glycosyltransferases for in situ nucleotide sugar regeneration, enabling ...
Tuan Son Hoang, Fabian Lange, Sebastian Bruno Kleeberg, Lea Thomas, Nam-Hai Hoang, Udo Reichl, Thomas F. T. Rexer   +6 more
wiley   +2 more sources

A Synergistic Inhibitor Development Strategy Against Human UDP‐Galactose‐4‐Epimerase [PDF]

Angewandte Chemie International Edition, Volume 65, Issue 22, 25 May 2026.
The epimerase GalE is crucial for the biosynthesis of cancer‐relevant O‐GalNAc glycans. Here, we employ orthogonal, structurally enabled small molecule fragment screens to yield both covalent and non‐covalent inhibitors against GalE within no more than 22 elaborated compounds.
William M. Browne, Jonathan Pettinger, Teresa Weckwerth, Andrew Purkiss, Sing Hei Lok, Louisa Penicaut, Roksana Ogrodowicz, Raveena Prema, Simone Kunzelmann, Chloe Roustan, Ganka Bineva‐Todd, Saskia Pieters, Francesca Zappacosta, Alfred E. Doherty, Isobel Oram, Christelle Soudy, Robert Quinlan, Joanna Redmond, Svend Kjaer, David House, Stephane Mouilleron, Jacob T. Bush, Benjamin Schumann   +22 more
wiley   +2 more sources

Mass Spectrometry Imaging‐Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa [PDF]

Advanced Science, Volume 13, Issue 29, 22 May 2026.
An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Yushi Liu, Wenna Duan, Weiwei Tang, Yucheng Zhao, Bin Li   +4 more
wiley   +2 more sources

Ganglioside biosynthesis. Characterization of uridine diphosphate galactose: GM2 galactosyltransferase in Golgiapparatus from rat liver

Journal of Lipid Research, 1976
An enzyme that transfers galactose from UDP-Gal to ganglioside GM2 (Tay-Sachs ganglioside) was concentrated 50 times in Golgi apparatus from rat liver relative to total homogenates.
F E Wilkinson, D J Morré, T W Keenan
doaj   +1 more source

Profiling of intracellular metabolites produced from galactose and its potential for galactosemia research

Orphanet Journal of Rare Diseases, 2018
Background Clinical outcome of patients with a classical presentation of galactosemia (classical patients) varies substantially, even between patients with the same genotype.
Michel van Weeghel, Lindsey Welling, Eileen P. Treacy, Ronald J. A. Wanders, Sacha Ferdinandusse, Annet M. Bosch   +5 more
doaj   +1 more source

Urinary reducing substances in neonatal intrahepatic cholestasis caused by citrin deficiency

Journal of Pediatric and Neonatal Individualized Medicine, 2014
Neonatal cholestasis due to citrin deficiency is an autosomal recessive metabolic disorder caused by mutations in SLC25A13 gene. Mutations in this gene have a relatively high prevalence in East-Asian races compared to European or Afro-Caribbean races ...
Ajmal Kader, Christina Ong, Veena Logarajah, Kong Boo Phua, Ee Shien Tan   +4 more
doaj   +1 more source

Overelaborated synaptic architecture and reduced synaptomatrix glycosylation in a Drosophila classic galactosemia disease model

Disease Models & Mechanisms, 2014
Classic galactosemia (CG) is an autosomal recessive disorder resulting from loss of galactose-1-phosphate uridyltransferase (GALT), which catalyzes conversion of galactose-1-phosphate and uridine diphosphate (UDP)-glucose to glucose-1-phosphate and UDP ...
Patricia Jumbo-Lucioni, William Parkinson, Kendal Broadie   +2 more
doaj   +1 more source

Identification of Leishmania major UDP-Sugar Pyrophosphorylase Inhibitors Using Biosensor-Based Small Molecule Fragment Library Screening

Molecules, 2019
Leishmaniasis is a neglected disease that is caused by different species of the protozoan parasite Leishmania, and it currently affects 12 million people worldwide.
Ohm Prakash, Jana Führing, John Post, Sharon M. Shepherd, Thomas C. Eadsforth, David Gray, Roman Fedorov, Françoise H. Routier   +7 more
doaj   +1 more source

Do Galactolipid Synthases Play a Key Role in the Biogenesis of Chloroplast Membranes of Higher Plants?

Frontiers in Plant Science, 2018
A unique feature of chloroplasts is their high content of the galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), which constitute up to 80% of their lipids.
Joana Rocha, Joana Rocha, Milène Nitenberg, Milène Nitenberg, Agnès Girard-Egrot, Juliette Jouhet, Juliette Jouhet, Eric Maréchal, Eric Maréchal, Maryse A. Block, Maryse A. Block, Christelle Breton, Christelle Breton   +12 more
doaj   +1 more source