Results 11 to 20 of about 66,671 (164)

Cell-Free Multi-Enzyme Synthesis and Purification of Uridine Diphosphate Galactose. [PDF]

Chembiochem, 2022
AbstractHigh costs and low availability of UDP‐galactose hampers the enzymatic synthesis of valuable oligosaccharides such as human milk oligosaccharides. Here, we report the development of a platform for the scalable, biocatalytic synthesis and purification of UDP‐galactose.
Mahour R, Lee JW, Grimpe P, Boecker S, Grote V, Klamt S, Seidel-Morgenstern A, Rexer TFT, Reichl U.   +8 more
europepmc   +5 more sources

Harnessing two UDP-glycosyltransferases from Saponaria officinalis toward heterologous production of endosomal escape enhancer saponins in Gypsophila elegans hairy roots. [PDF]

Plant J
SUMMARY Soapwort (Saponaria officinalis) synthesizes a vast repertoire of specialized metabolites with bioactive properties. Among those, a specific type of highly glycosylated oleanane‐derived triterpenoid saponins has potential applications in the pharmaceutical industry due to their anticancer activity and enhancement of endosomal escape for ...
Qu T, Schinkel H, Moses T, Reddy K, de Vaumas R, Schillberg S, Goossens A, Lacchini E.   +7 more
europepmc   +2 more sources

Osmolality-Independent Impact of Sodium on Glycosylation of an Fc-Fusion Protein and the Hexosamine Biosynthesis Pathway in a Chinese Hamster Ovary Cell Line. [PDF]

Biotechnol J
Graphical Abstract and Lay Summary Sodium and potassium supplementation in CHO batch culture elevates intracellular UDP‐HexNAc concentrations through activation of the hexosamine biosynthesis pathway (HBP), independently of osmolality. Increased nucleotide sugar availability enhances Fc‐fusion protein glycosylation and reduces the abundance of low ...
Reiniger J, Pöttner A, Rosenberger L, Zimmer A.   +3 more
europepmc   +2 more sources

The enzymatic transformation of uridine diphosphate glucose into a galactose derivative [PDF]

Archives of Biochemistry and Biophysics, 1951
Abstract Treatment of uridine diphosphate glucose (UDPG) with an enzyme of S. fragilis was found to produce about 25% of a galactose-containing compound. This compound is precipitated with mercuric ions like UDPG, and its migration in chromatography in acid-ethanol is similar.
Luis Federico Leloir, L F Leloir
exaly   +4 more sources

Uridine Diphosphate Glucose: the Coenzyme of the Galactose–Glucose Phosphate Isomerization [PDF]

Nature, 1950
A PREVIOUS report1 dealt with the enzymatic conversion of galactose-1-phosphate into glucose-1-phosphate and mentioned that a thermostable factor is necessary for the reaction. This factor has now been obtained practically pure, and for reasons given below will be referred to as uridine diphosphate glucose.
R Caputto, L F Leloir
exaly   +3 more sources

Multienzyme Platform for the Synthesis of UDP Sugars and Human Milk Oligosaccharides. [PDF]

Chembiochem
Engineered Escherichia coli produces six enzymes (UMPK, PPK3, GALK, NAHK, GALU, PPA) that are purified and used in a cell‐free cascade to synthesize UDP‐N‐acetylglucosamine (UDP‐GlcNAc) and UDP‐galactose (UDP‐Gal) from inexpensive substrates. The cascades can be coupled with glycosyltransferases for in situ nucleotide sugar regeneration, enabling ...
Hoang TS, Lange F, Kleeberg SB, Thomas L, Hoang NH, Reichl U, Rexer TFT.   +6 more
europepmc   +2 more sources

Review of Congenital Myasthenic Syndrome Caused by Pathogenic Variants in GFPT1. [PDF]

Muscle Nerve
ABSTRACT Glutamine:fructose‐6‐phosphate transaminase 1 (GFPT1) catalyzes the first and rate‐limiting step of the hexosamine biosynthetic pathway (HBP) to generate UDP‐GlcNAc. GFPT1 exon 9 is specifically spliced in in striated muscles, which makes a long isoform of GFPT1 (GFPT1‐L).
Ohno K, Nazim M, Zhang R, Farshadyeganeh P, Ito M, Ohkawara B.   +5 more
europepmc   +2 more sources

A Synergistic Inhibitor Development Strategy Against Human UDP-Galactose-4-Epimerase. [PDF]

Angew Chem Int Ed Engl
The epimerase GalE is crucial for the biosynthesis of cancer‐relevant O‐GalNAc glycans. Here, we employ orthogonal, structurally enabled small molecule fragment screens to yield both covalent and non‐covalent inhibitors against GalE within no more than 22 elaborated compounds.
Browne WM, Pettinger J, Weckwerth T, Purkiss A, Lok SH, Penicaut L, Ogrodowicz R, Prema R, Kunzelmann S, Roustan C, Bineva-Todd G, Pieters S, Zappacosta F, Doherty AE, Oram I, Soudy C, Quinlan R, Redmond J, Kjaer S, House D, Mouilleron S, Bush JT, Schumann B.   +22 more
europepmc   +2 more sources

Mass Spectrometry Imaging-Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa. [PDF]

Adv Sci (Weinh)
An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Liu Y, Duan W, Tang W, Zhao Y, Li B.
europepmc   +2 more sources

Structural Studies of Catalysis and Processing in Galactose [PDF]

, 2007
The fungal copper containing enzyme galactose oxidase is one of the earliest examples of a protein derived radical cofactor. A covalent bond, formed between the active site cysteine and the Cc of a tyrosine act as a site for radical formation, allowing ...
Akumanyi, Nana
core   +7 more sources