Rates of UMP-dependent reduction of the DPN+ associated with Escherichia coli UDP-galactose-4-epimerase at 27 degrees and 0.2 M ionic strength in 0.1 M Tris-HCl buffer, pH 8.5, are reported. The reaction exhibits excellent pseudo-first order behavior when D-glucose is at anomeric equilibrium.
Unwoo Kang, Lynn D. Nolan, Perry A. Frey
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Uridine diphosphate glucose and uridine diphosphate galactose in galactosemia
Michael Palmieri +3 more
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Induced Reoxidation and Reactivation of a Reduced Uridine Diphosphate Galactose-4-epimerase Complex
Abstract An inactive reduced UDP-galactose-4-epimerase complex, which is prepared by reduction with NaBH4 in the presence of substrates and is known to contain DPNH and UDP-hexoses in tightly bound form, is largely reoxidized and partially reactivated in the presence of 0.02 to 0.4 m cyclohexanone or cyclohexanol.
Timple G. Wee, Perry A. Frey
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Computer-aided identification of Trypanosoma brucei uridine diphosphate galactose 4'-epimerase inhibitors: toward the development of novel therapies for African sleeping sickness. [PDF]
Durrant JD +3 more
europepmc +3 more sources
Galactosemia caused by generalized uridine diphosphate galactose-4-epimerase deficiency
Luigi Garibaldi +2 more
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Structural characterisation of nucleotide sugar short-chain dehydrogenases/reductases from the thermophilic pseudomurein-containing methanogen Methanothermobacter thermautotrophicus ΔH. [PDF]
Nucleotide sugar short‐chain dehydrogenases/reductases (NS‐SDRs) are involved in pseudomurein and capsular polysaccharide formation in methanogenic Archaea. Methanothermobacter thermautotrophicus possesses several NS‐SDRs labelled Mth375, Mth380, Mth373, Mth631 and Mth1789.
Carbone V +4 more
europepmc +2 more sources
Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures. [PDF]
Abstract Objective Malformations of cortical development represent major causes of drug‐resistant epilepsy, with mild malformation of cortical development with oligodendroglial hyperplasia and epilepsy recognized as a distinct pathological entity. Pathogenic X‐linked SLC35A2, encoding the uridine diphosphate–galactose transporter, has been implicated ...
Bartel TM +6 more
europepmc +2 more sources
Uridine Diphosphate Galactose 4-Epimerase from Yeast
Robert A. Darrow, Robert Rodstrom
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In order to clarify the pathogenic function of uridine diphosphate (UDP)-glucose 4-epimerase encoding gene galE on Ralstonia pseudosolanacearum, the association between galE and the pathogenic-related phenotypes, and the gene deletion effect on ...
ZHANG Hong +8 more
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Uridine diphosphate glucose and uridine diphosphate galactose in galactosemia
Henry N. Kirkman
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