Results 91 to 100 of about 57,494 (245)

Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin

Food and Agricultural Immunology, 2020
Competitive enzyme-linked immunosorbent assay (ELISA) is the most widely used method for rapidly screening of mycotoxins in contaminated food products; however, it involves the use of toxin-protein conjugates as a competitive antigen and toxin standards ...
Liang Xiong, Xueqin Zhang, Yang Xu, Yanping Li, Deguang Liu, Zhui Tu, Qinghua He   +6 more
doaj   +1 more source

Functional Divergence in the Affinity and Stability of Non-Canonical Cysteines and Non-Canonical Disulfide Bonds: Insights from a VHH and VNAR Study

International Journal of Molecular Sciences
Single-domain antibodies, including variable domains of the heavy chains of heavy chain-only antibodies (VHHs) from camelids and variable domains of immunoglobulin new antigen receptors (VNARs) from cartilaginous fish, show the therapeutic potential of ...
Mingce Xu, Zheng Zhao, Penghui Deng, Mengsi Sun, Cookson K. C. Chiu, Yujie Wu, Hao Wang, Yunchen Bi   +7 more
semanticscholar   +1 more source

CD3ɛ Nanobody‐Engineered Extracellular Vesicles Driving In Vivo Generation of TCE‐secreting CAR‐Ts for Solid Tumor Therapy With Memory Response and Minimal Immunogenicity

Advanced Science, EarlyView.
HEK‐293T‐derived CD3ε Nb‐engineered EVs to generate dual‐targeting CAR‐T cells directly in vivo. These EVs selectively deliver CAR.BiTE transgenes into T cells and reprogramed to HLA‐G/PD‐L1‐targeting effector cells with enhanced memory and persistence.
Shi‐Wei Huang, Yu‐Chuan Lin, Chih‐Ming Pan, Yeh Chen, Ming‐You Shie, Cheng‐Yu Chen, Kai‐Wen Kan, Yi‐Wen Chen, Ming‐Chao Liu, Chung‐Chun Wu, Yu‐Ting Chiang, Huai‐Ping Ho, Chen‐Yu Lin, Pei‐Ying Lin, Yu‐Han Huang, Steffany Rusli, Wan‐Yu Mao, Pei‐Wen Huang, Sin‐Ting Wang, Wan‐Chen Tsai, Ya‐Hsu Chiu, Ting‐Hsun Lin, Wan‐Ling Chiang, Che‐Kai Chang, Zi‐Lun Lai, Mei‐Chih Chen, Shao‐Chih Chiu, Der‐Yang Cho   +27 more
wiley   +1 more source

Anticuerpos de cadena única de alpaca para la detección de antígenos de Fasciola hepatica

Revista Peruana de Medicina Experimental y Salud Pública, 2018
Objetivo. Producir anticuerpos recombinantes de cadena única de alpaca que se unan con alta afinidad y especificidad al antígeno excretado-secretado (ES) de Fasciola hepatica para el desarrollo de tecnologías nuevas de diagnóstico de fascioliasis humana ...
Teresa Barreto, Yenisey Alfonso, Pierre Lafaye, Maria del Pilar García Lazaro, L. Agueda Perez, Patricia Herrera-Velit, Jose R. Espinoza   +6 more
doaj   +1 more source

Development of a highly specific and sensitive VHH-based sandwich immunoassay for the detection of the SARS-CoV-2 nucleoprotein

Journal of Biological Chemistry, 2021
The current COVID-19 pandemic illustrates the importance of obtaining reliable methods for the rapid detection of SARS-CoV-2. A highly specific and sensitive diagnostic test able to differentiate the SARS-CoV-2 virus from common human coronaviruses is ...
Marion Gransagne, G. Aymé, S. Brier, Gaëlle Chauveau-Le Friec, Véronique Mériaux, M. Nowakowski, F. Déjardin, S. Levallois, Guilherme Dias de Melo, F. Donati, M. Prot, Sébastien Brûlé, B. Raynal, J. Bellalou, P. Gonçalves, X. Montagutelli, J. D. Di Santo, F. Lazarini, P. England, S. Petres, N. Escriou, P. Lafaye   +21 more
semanticscholar   +1 more source

Cationic mRNA Lipid Nanoparticles for Ex Vivo NanoCAR‐T Cell Engineering

Advanced Science, EarlyView.
This study compares charge‑neutral and cationic lipidnanoparticles (LNPs) for ex vivo mRNA delivery to T cells, revealing distinctdependencies on medium composition and T cell activation. Where conventional LNPs benefit from ApoE‐dependent T cell targeting, cationic DOTAP‑modified LNPs mediate unspecific charge‑dependent transfection.
Laure Harinck, Stijn De Munter, Margo De Velder, Joline Ingels, Dominika Berdecka, Ine Lentacker, Winnok H. De Vos, Bart Vandekerckhove, Kevin Braeckmans, Koen Raemdonck   +9 more
wiley   +1 more source

Delicate balance among thermal stability, binding affinity, and conformational space explored by single-domain VHH antibodies

Scientific Reports, 2021
The high binding affinities and specificities of antibodies have led to their use as drugs and biosensors. Single-domain VHH antibodies exhibit high specificity and affinity but have higher stability and solubility than conventional antibodies as they ...
Emina Ikeuchi, Daisuke Kuroda, Makoto Nakakido, A. Murakami, K. Tsumoto   +4 more
semanticscholar   +1 more source

Lifelong Evolution of Autoreactive Plasma Cell Numbers, Affinity, and Anatomic Location in Arthritic K/BxN Mice

Arthritis &Rheumatology, EarlyView.
Objective The spontaneous K/BxN mouse model of rheumatoid arthritis has been used extensively to study chronic inflammation, contribution of immune cells, and the primordial role of autoreactive antibodies in disease initiation and severity. Only the ubiquitous enzyme glucose‐6‐phosphate isomerase (GPI) is the target of IgG autoantibodies secreted by ...
Thibault Vanhoucke, Carlos Castrillon, Odile Richard‐Le Goff, Angga Perima, Matteo Broketa, Andrew D. Griffiths, Patrick England, François Huetz, Pierre Bruhns   +8 more
wiley   +1 more source

Super Potent Bispecific Llama VHH Antibodies Neutralize HIV via a Combination of gp41 and gp120 Epitopes

Antibodies, 2019
Broad and potent neutralizing llama single domain antibodies (VHH) against HIV-1 targeting the CD4 binding site (CD4bs) have previously been isolated upon llama immunization.
Nika M. Strokappe, Miriam Hock, Lucy Rutten, Laura E. Mccoy, Jaap W. Back, Christophe Caillat, Matthias Haffke, Robin A. Weiss, Winfried Weissenhorn, Theo Verrips   +9 more
doaj   +1 more source

Structural Evaluation of EGFR Inhibition Mechanisms for Nanobodies/VHH Domains [PDF]

Structure, 2013
The epidermal growth factor receptor (EGFR) is implicated in human cancers and is the target of several classes of therapeutic agents, including antibody-based drugs. Here, we describe X-ray crystal structures of the extracellular region of EGFR in complex with three inhibitory nanobodies, the variable domains of heavy chain only antibodies (VHH).
Schmitz, K.R., Bagchi, A., Roovers, R.C., van Bergen en Henegouwen, P.M.P., Ferguson, K.M.   +4 more
openaire   +3 more sources