Results 81 to 90 of about 58,013 (257)

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Development of novel humanized VHH synthetic libraries based on physicochemical analyses

Scientific Reports
Due to the high affinity and specificity of antibodies toward antigens, various antibody-based applications have been developed. Recently, variable antigen-binding domains of heavy-chain antibodies (VHH) have become an attractive alternative to ...
Makoto Nakakido, Seisho Kinoshita, Kouhei Tsumoto   +2 more
semanticscholar   +1 more source

VHH Structural Modelling Approaches: A Critical Review

International Journal of Molecular Sciences, 2022
VHH, i.e., VH domains of camelid single-chain antibodies, are very promising therapeutic agents due to their significant physicochemical advantages compared to classical mammalian antibodies.
Poonam Vishwakarma, Akhila Melarkode Vattekatte, Nicolas K. Shinada, J. Diharce, Carla Martins, F. Cadet, F. Gardebien, C. Etchebest, A. Nadaradjane, A. D. de Brevern   +9 more
semanticscholar   +1 more source

Distinct types of VHHs in Alpaca

Frontiers in Immunology
IntroductionVHHs (VH of heavy-chain-only antibodies) represent a unique alternative to Q7 conventional antibodies because of their smaller size, comparable binding affinity and biophysical properties. MethodIn this study, we systematically analyzed VHH NGS sequences from 22 Alpacas and structure data from public database.
Xinhao Wang, Lu Zhang, Yao Zhang, Jiaguo Li, Wenfeng Xu, Weimin Zhu   +5 more
openaire   +3 more sources

GPCRs in CAR‐T Cell Immunotherapy: Expanding the Target Landscape and Enhancing Therapeutic Efficacy

Advanced Science, EarlyView.
Chimeric antigen receptor T cell therapy faces dual challenges of target scarcity and an immunosuppressive microenvironment in solid tumors. This review highlights how G protein‐coupled receptors can serve as both novel targets to expand the therapeutic scope and functional modules to enhance CAR‐T cell efficacy.
Zhuoqun Liu, Yuchen Xiao, Yamin Zhao, Lihe Dai, David J.H. Shih, Shikang Liang, Honglei Tian, Liu Liu, Feng Tian, Bing Liu, Lei Chang, Chao Zhang   +11 more
wiley   +1 more source

Novel VHH-Based Tracers with Variable Plasma Half-Lives for Imaging of CAIX-Expressing Hypoxic Tumor Cells

Molecular Pharmaceutics, 2022
Hypoxic areas are present in the majority of solid tumors, and hypoxia is associated with resistance to therapies and poor outcomes. A transmembrane protein that is upregulated by tumor cells that have adapted to hypoxic conditions is carbonic anhydrase ...
S. V. van Lith, F. Huizing, G. Franssen, B. Hoeben, J. Lok, S. Doulkeridou, O. Boerman, M. Gotthardt, P. M. P. van Bergen en Henegouwen, J. Bussink, S. Heskamp   +10 more
semanticscholar   +1 more source

Anticuerpos de cadena única de alpaca para la detección de antígenos de Fasciola hepatica

Revista Peruana de Medicina Experimental y Salud Pública, 2018
Objetivo. Producir anticuerpos recombinantes de cadena única de alpaca que se unan con alta afinidad y especificidad al antígeno excretado-secretado (ES) de Fasciola hepatica para el desarrollo de tecnologías nuevas de diagnóstico de fascioliasis humana ...
Teresa Barreto, Yenisey Alfonso, Pierre Lafaye, Maria del Pilar García Lazaro, L. Agueda Perez, Patricia Herrera-Velit, Jose R. Espinoza   +6 more
doaj   +1 more source

Divergent Roles of mGlu2 and mGlu3 Receptors in Amyloid‐β Production and Cognitive Dysfunctions in Alzheimer's Disease

Advanced Science, EarlyView.
This study explores the opposing effects of the mGluR2 and mGluR3 receptors on amyloid precursor protein processing. mGluR2 promotes amyloidogenic cleavage, while mGluR3 favors non‐amyloidogenic pathways. Using a brain‐penetrant nanobody as a mGluR2 positive allosteric modulator, the study uncovers how its chronic activation aggravates amyloid‐β burden
Pierre‐André Lafon, Mireille Elodie Tsitokana, Ugo Guy Alenda, Yen‐Ling Lian, Clémentine Eva Philibert, Mathieu Oosterlaken, Marta Cimadevila, Gaëlle Dudon, Jessica Monnic, Salomé Roux, Julie Bessié, Séverine Diem, Franck Vandermoere, Laurent Prézeau, Patrick Chames, Julie Kniazeff, Sylvie Claeysen, Anaïs Menny, Jean‐Philippe Pin, Véronique Perrier, Jianfeng Liu, Philippe Rondard   +21 more
wiley   +1 more source

Single VHH-directed BCMA CAR-T cells cause remission of relapsed/refractory multiple myeloma

Leukemia, 2021
Chimeric antigen receptor T (CAR-T) cells have shown remarkable effects in treating hematological malignancies [1, 2]. Typically, the antigen recognition domain of CAR-T cells is a single-chain variable fragment (scFv) linked to a costimulatory domain ...
Lu Han, Jishuai Zhang, Jian Zhou, K. Zhou, Ben-Ling Xu, Lin-Lin Li, B. Fang, Q. Yin, Xing-hu Zhu, Hu Zhou, Xudong Wei, Hong-Chang Su, Bingxiang Zhang, Ya-Nan Wang, B. Xiang, Quanli Gao, Yong-Ping Song   +16 more
semanticscholar   +1 more source

Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin

Food and Agricultural Immunology, 2020
Competitive enzyme-linked immunosorbent assay (ELISA) is the most widely used method for rapidly screening of mycotoxins in contaminated food products; however, it involves the use of toxin-protein conjugates as a competitive antigen and toxin standards ...
Liang Xiong, Xueqin Zhang, Yang Xu, Yanping Li, Deguang Liu, Zhui Tu, Qinghua He   +6 more
doaj   +1 more source