Results 111 to 120 of about 57,494 (245)

Overexpression, Purification, Characterization, and Pathogenicity of Vibrio harveyi Hemolysin VHH [PDF]

Infection and Immunity, 2006
ABSTRACT Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli , and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity ...
Zhong, Yingbin, Zhang, Xiao-Hua, Chen, Jixiang, Chi, Zhenghao, Sun, Boguang, Li, Yun, Austin, Brian   +6 more
openaire   +4 more sources

Nanobody–chlorin e6 conjugate for Nectin‐4‐mediated tumor targeting and enhanced photodynamic therapy

VIEW, EarlyView.
Nectin‐4 is frequently overexpressed in solid tumors, yet many PDT photosensitizers show poor water solubility and nonspecific uptake. A Nectin‐4‐targeted nanobody–chlorin e6 conjugate (NPC) improves aqueous solubility and selectively accumulates in Nectin‐4‐positive tumor cells.
Haodian Wan, Tao Ye, Yawei Bi, Jiayu Zhang, Wei Wu, Aochi Liu, Li Sun, Jie Lin, Ningli Chai, Manjie Zhang, Jian Ma   +10 more
wiley   +1 more source

Cloning and Expression of Nano Body Gene against Enterotoxin B of Staphylococcus Aureus [PDF]

Journal of Advanced Biomedical Sciences, 2016
Background & Objectives: Staphylococcus aureus bacteria causes many different diseases by secretion of various enterotoxins. Therefore, it is necessary to develop ways that facilitate the detection of enterotoxins.
Zahra Tavassoli, Hamideh Rouhani Nejad, Jalil Fallah Mehrabadi   +2 more
doaj  

Anti-VP6 VHH: An Experimental Treatment for Rotavirus A-Associated Disease. [PDF]

PLoS ONE, 2016
Species A Rotaviruses (RVA) remain a leading cause of mortality in children under 5 years of age. Current treatment options are limited. We assessed the efficacy of two VP6-specific llama-derived heavy chain antibody fragments (VHH) -2KD1 and 3B2- as an ...
Lucía Maffey, Celina G Vega, Samuel Miño, Lorena Garaicoechea, Viviana Parreño   +4 more
doaj   +1 more source

A new catfish species of Microcambeva Costa & Bockmann, 1994 (Siluriformes: Trichomycteridae) from southern Brazil, with a redescription of M. ribeirae Costa, Lima & Bizerril, 2004

Journal of Fish Biology, EarlyView.
Abstract Among the few species of Microcambeva reported as occurring in more than one hydrographic basin, M. ribeirae has been previously listed from both the Ribeira de Iguape and the Guaraqueçaba basins. However, morphological and molecular analyses revealed that the specimens from Guaraqueçaba represent a new species, which is described in this ...
Lucas S. de Medeiros, Igor C. A. Souto‐Santos, Paulo A. Buckup, Juliano Ferrer, Vinicius J. C. Reis, Mario de Pinna, Sergio M. Q. Lima   +6 more
wiley   +1 more source

PCR detection of hemolysin (vhh) gene in Vibrio harveyi

The Journal of General and Applied Microbiology, 2004
The Vibrio harveyi hemolysin gene (vhh), which encodes for a virulence factor involved in pathogenicity to fish and shellfish species, may be targeted for species detection or strain differentiation. Primers designed for this gene were used in detection studies of V. harveyi strains from various hosts.
Mia Judith U, Conejero, Cynthia T, Hedreyda   +1 more
openaire   +3 more sources

Rational Design of Nanobodies Targeting LINGO‐1

Applied Research, Volume 5, Issue 2, April 2026.
We rationally designed and recombinantly produced a panel of single‐domain antibodies (nanobodies) targeting the human transmembrane protein LINGO‐1, a key player in multiple sclerosis. ABSTRACT The leucine‐rich repeat and immunoglobulin‐like domain‐containing Nogo receptor‐interacting protein 1 (LINGO‐1) is a transmembrane protein expressed in neurons
Alexander Röntgen, Vaidehi Roy Chowdhury, Aubin Ramon, Matthew Greenig, Xing Xu, Pietro Sormanni, Michele Vendruscolo   +6 more
wiley   +1 more source

Nanobody‐Mediated c‐MYC Degradation Inhibits Tumor Cell Progression

MedComm, Volume 7, Issue 4, April 2026.
A cell‐permeable nanobody strategy was developed to target the intrinsically disordered oncoprotein c‐MYC. A synthetic nanobody, M4, binds the central PEST region of c‐MYC (241–263) and promotes c‐MYC degradation by enhancing Thr58 phosphorylation. Conjugation of M4 to a cell‐penetrating peptide (CPP) generates CPM4, which efficiently accumulates in ...
Yuanyuan Xue, Hao Jiang, Zhaoyun Zong, Xiaolin Tian, Zelong Miao, Ting Li, Yali Wei, Haiteng Deng   +7 more
wiley   +1 more source

Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform

mAbs, 2020
Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a conventional antibody by two independently functioning VHH domains. Consequently,
Lukas Pekar, Michael Busch, Bernhard Valldorf, Steffen C. Hinz, Lars Toleikis, Simon Krah, Stefan Zielonka   +6 more
doaj   +1 more source

Non‐Viral Cytokine‐Inducible SH2 Containing Protein Locus‐Specific Integrated Fibroblast Activation Protein Alpha‐Targeting Chimeric Antigen Receptor T Cells Achieve Potent Antitumor Efficacy in Glioblastoma

MedComm, Volume 7, Issue 4, April 2026.
FAPα is a dual target on glioblastoma cells and perivascular cells. Using a high‐affinity anti‐FAPα nanobody, we generated CISH‐KO FAPα‐CAR‐T cells via non‐viral CRISPR–Cas9 integration. These cells demonstrated enhanced proliferation and superior antitumor efficacy in patient‐derived organoids and orthotopic mouse models compared to conventional CAR‐T
Xin Dong, Yao Sun, Yuetong Guo, Jiao Wang, Fei Wang, Ziming Wang, Ruizhen Li, Fei Xie, Tingting Tan, Baijie Cheng, Ronghan Huang, Shu Zhang, Xiaotong Lin, Zhaoze Guo, Hubing Wu, Hao Wu, Xubiao Zhang, Guozhu Xie   +17 more
wiley   +1 more source