Association of rotavirus viroplasms with microtubules through NSP2 and NSP5 [PDF]
Memorias Do Instituto Oswaldo Cruz, 2006 Rotavirus replication and virus assembly take place in electrodense spherical structures known as viroplasms whose main components are the viral proteins NSP2 and NSP5.
Claudio Cabral-Romero
exaly +6 more sources
Rotavirus Spike Protein VP4 Mediates Viroplasm Assembly by Association to Actin Filaments. [PDF]
J Virol, 2022 Rotavirus (RV) viroplasms are cytosolic inclusions where both virus genome replication and primary steps of virus progeny assembly take place. A stabilized microtubule cytoskeleton and lipid droplets are required for the viroplasm formation, which ...
Vetter J +9 more
europepmc +4 more sources
Conserved rotavirus NSP5 and VP2 domains interact and affect viroplasm [PDF]
Journal of Virology, 2020 One step of the life cycle common among all rotaviruses (RV) studied so far is the formation of viroplasms, membrane-less cytosolic inclusions providing a microenvironment for early morphogenesis and RNA replication.
Ackermann, Mathias +5 more
core +4 more sources
Characterization of viroplasm formation during the early stages of rotavirus infection [PDF]
Virology Journal, 2010 Background During rotavirus replication cycle, electron-dense cytoplasmic inclusions named viroplasms are formed, and two non-structural proteins, NSP2 and NSP5, have been shown to localize in these membrane-free structures.
Isa Pavel +4 more
doaj +3 more sources
Rotavirus viroplasm fusion and perinuclear localization are dynamic processes requiring stabilized microtubules. [PDF]
PLoS ONE, 2012 Rotavirus viroplasms are cytosolic, electron-dense inclusions corresponding to the viral machinery of replication responsible for viral template transcription, dsRNA genome segments replication and assembly of new viral cores. We have previously observed
Catherine Eichwald +7 more
doaj +6 more sources
Plasmid-Based Reverse Genetics for Probing Phosphorylation-Dependent Viroplasm Formation in Rotaviruses [PDF]
Virus Research, 2020 Rotavirus (RV) replication occurs in cytoplasmic compartments, known as viroplasms, that are composed of viral and cellular proteins. Viroplasm formation requires RV nonstructural proteins NSP2 and NSP5 and cellular lipid droplets (LDs); however, the ...
Crawford, Sue E +2 more
core +6 more sources
Significance of Cellular Lipid Metabolism for the Replication of Rotaviruses and Other RNA Viruses [PDF]
VirusesThe replication of species A rotaviruses (RVAs) involves the recruitment of and interaction with cellular organelles’ lipid droplets (LDs), both physically and functionally.
Ulrich Desselberger
doaj +2 more sources
Rotavirus NSP2: A Master Orchestrator of Early Viral Particle Assembly [PDF]
VirusesRotaviruses (RVs) are 11-segmented, double-stranded (ds) RNA viruses and important causes of acute gastroenteritis in humans and other animal species.
Sarah L. Nichols +3 more
doaj +2 more sources
A Fijivirus Major Viroplasm Protein Shows RNA-Stimulated ATPase Activity by Adopting Pentameric and Hexameric Assemblies of Dimers [PDF]
mBio, 2023 Fijiviruses replicate and package their genomes within viroplasms in a process involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein of the mal de Río Cuarto virus
Gabriela Llauger +18 more
doaj +5 more sources
Host Factor Requirements of the Rotavirus Viroplasm [PDF]
Rotavirus (RV) is the primary etiological agent responsible for severe gastroenteritis and dehydration worldwide in infants and young animals. RV virions are icosahedral, non-enveloped particles with three concentric layers of protein (TLP, triple ...
Vetter, Janine
core +3 more sources