Results 81 to 90 of about 45,419 (204)

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Feline calicivirus can tolerate gross changes of its minor capsid protein expression levels induced by changing translation reinitiation frequency or use of a separate VP2-coding mRNA. [PDF]

PLoS ONE, 2014
Caliciviruses use reinitiation of translation governed by a 'termination upstream ribosomal binding site' (TURBS) for expression of their minor capsid protein VP2.
Maria Haβ, Christine Luttermann, Gregor Meyers   +2 more
doaj   +1 more source

The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein.

PLoS ONE, 2021
There are a wide variety of porcine parvoviruses (PPVs) referred to as PPV1 to PPV7. The latter was discovered in 2016 and later reported in some countries in America, Asia, and Europe.
Diana S Vargas-Bermudez, Santiago Rendon-Marin, Julian Ruiz-Saenz, Dario Mogollón, Jairo Jaime   +4 more
doaj   +1 more source

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

Exchange of the C-terminal part of VP3 from very virulent infectious bursal disease virus results in an attenuated virus with a unique antigenic structure

, 2002
Infectious bursal disease virus (IBDV) is the major viral pathogen in the poultry industry. Live attenuated serotype 1 vaccine strains are commonly used to protect susceptible chickens during their first 6 weeks of life. Wild-type serotype 1 IBDV strains
Peeters, B.P.H., ter Huurne, A.A.H.M., Pol, J.M., Hoekman, A.J.W., Huurne, A.A.H.M., ter, Boot, H.J., Gielkens, A.L.J.   +6 more
core   +1 more source

Molecular characterization of porcine parvovirus 1 based on partial VP2 gene in the ovaries of Thai pigs

Veterinary Integrative Sciences, 2022
Porcine parvovirus 1 (PPV1) is the causative agent of swine reproductive disease, particularly in naive gilts and sows. This study aimed to investigate the prevalence and genetic diversity of the partial nucleotide sequence of the VP2 gene and ...
Prayuth Saekhow, Chayaphon Sriphannam, Panuwat Yamsakul   +2 more
doaj   +1 more source

Iflaviruses in arthropods: when small is mighty

Insect Science, EarlyView.
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia, Hannah‐Isadora Huditz, Monique M. van Oers, Vera I.D. Ros   +3 more
wiley   +1 more source

Expanding the Fish‐Brain Invitrome With the Senegalese Sole SsB‐1 Cell Line—A Versatile Model for Neurotropic Virus Research

Journal of Fish Diseases, EarlyView.
ABSTRACT Cell lines are essential tools for studying animal physiology and immunopathology, reducing the use of live animals and supporting the 3Rs principle of reduction, refinement, and replacement. The Senegalese sole (Solea senegalensis), a relevant species for Spanish aquaculture diversification, lacks established in vitro models. We developed SsB‐
Yulema Valero, Alberto Cuesta
wiley   +1 more source

Expression of immunogenic VP2 protein of infectious bursal disease virus in Arabidopsis thaliana [PDF]

Biotechnology Letters, 2004
VP2 protein is the major host-protective immunogen of infectious bursal disease virus (IBDV) of chickens. Transgenic lines of Arabidopsis thaliana expressing recombinant VP2 were developed. The VP2 gene of an IBDV antigenic variant E strain was isolated, amplified by RT-PCR and introduced into a plant expression vector, pE1857, having a strong promoter
Wu, H., Singh, N.K., Locy, R.D., Scissum-Gunn, K., Giambrone, J.J.   +4 more
openaire   +2 more sources

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, Volume 101, Issue 7, Page 1384-1395, July 2026.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source