Results 181 to 190 of about 3,424,209 (206)

Modulation of walnut protein solubility: effects of multiple modification treatments on walnut protein structure, physicochemical properties, and investigation of mechanisms

Food Research International
In this study, we proposed a targeted modification technique of ultrasound-assisted restriction enzyme digestion combined with glycosylation, which could significantly improve the solubility of walnut protein (WP, +91.63 %). The mechanism was elucidated by spectral analysis and molecular docking: ultrasound changed the conformation of WP to promote ...
Shutong, Zhao, Qingzhi, Wu, Yijian, Jiang, Jialu, Li, Qi, Hao, Jian, Zhang, Xiaoying, Mao   +6 more
openaire   +2 more sources

Identification of Key Lipogenesis Stages and Proteins Involved in Walnut Kernel Development

Journal of Agricultural and Food Chemistry, 2023
Walnuts are abundant in oil content, especially for polyunsaturated fatty acids, but the understanding of their formation is limited. We collected walnut (Juglans regia L.) kernels at 60, 74, 88, 102, 116, 130, and 144 days after pollination (designated S1-S7).
Feng Jin, Ye Zhou, Pu Zhang, Ruimin Huang, Wei Fan, Baoxin Li, Guangzhu Li, Xiaobo Song, Dong Pei   +8 more
openaire   +2 more sources

Development of an indirect competitive immunoassay for walnut protein component in food

Food Chemistry, 2014
Among food allergens, walnut is a frequent cause of adverse food reactions in allergic patients. In this study, the walnut allergen protein 2S albumin precursor (Jug r 1) cDNA was synthesised and cloned into the pGEX-6P-1 expression vector. The recombinant plasmids were transformed into Escherichia coli (E.
Haiyan, Wang, Gang, Li, Yajun, Wu, Fei, Yuan, Ying, Chen   +4 more
openaire   +2 more sources

Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein

Journal of Agricultural and Food Chemistry, 2013
In the present study, a novel angiotensin I-converting enzyme (ACE)-inhibitory peptide, P-2a2, was purified to homogeneity from walnut protein hydrolysate by ultrafiltration, consecutive column chromatography, and high-performance liquid chromatography. The purified peptide was characterized by matrix-assisted laser desorption ionization time-of-flight
Meng, Liu, Ming, Du, Yingchun, Zhang, Weili, Xu, Cong, Wang, Kejian, Wang, Lanwei, Zhang   +6 more
openaire   +2 more sources

Quantitative indirect ELISA for determination of walnut proteins in foods

Food Science and Biotechnology, 2015
An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein.
Juan Fang, Dan Chen, Chaoyin Chen, Feng Ge, Diqiu Liu, Benyong Han, Xiangfeng Xiong   +6 more
openaire   +1 more source

Enzymolysis Optimization of Walnut Residue Protein Using Trypsase

Advanced Materials Research, 2013
The enzymolysis of walnut residue protein was investigated using trypsase, and the best process conditions were studied by one-factor experiment and orthogonal experiment. The result showed that the influential degree weakened as follows sequence: temperature, pH, enzyme quantity, and time.
Shao Ying Zhang, Yin Zhe Ren
openaire   +1 more source

Modulation of cecal microbiota and fecal metabolism in mice by walnut protein

Food & Function
The characteristic functional groups of WP exhibit obvious absorption peaks, with >30% α-helix and β-folding content in the secondary structure. WP is a typical spherical particle with a denaturation temperature of 73.6 °C, which indicates its good structural stability and thermal stability.
Huang Si, Wang Yan, Sheng Jun, Mu Hongyu, Zhang Xia, Wu Kuan, Zhao Cunchao   +6 more
openaire   +2 more sources

Optimization of protein extraction technique in walnut fruits

Acta Horticulturae, 2021
S.Yu. Khokhlov, E.S. Panyushkina, V.V. Vasipov, A.A. Petrov, E.G. Chupahin, I.V. Kruchina-Bogdanov, I.S. Gorbunov   +6 more
openaire   +1 more source

Phenolic compounds in walnut pellicle improve walnut ( L.) protein solubility under pH-shifting condition

Food Research International, 2023
Yeming Chen, Xingfei Li, Xiangzhen Kong
exaly  

Solubilization mechanism of self-assembled walnut protein nanoparticles and curcumin encapsulation

Journal of the Science of Food and Agriculture, 2023
Fengjun Wang
exaly