Results 101 to 110 of about 4,960 (183)

Pervasive translation of Xrn1-sensitive unstable long non-coding RNAs in yeast

RNA
Despite being predicted to lack coding potential, cytoplasmic long non-coding (lnc)RNAs can associate with ribosomes. However, the landscape and biological relevance of lncRNAs translation remains poorly studied. In yeast, cytoplasmic Xrn1-sensitive lncRNAs (XUTs) are targeted by the Nonsense-Mediated mRNA Decay (NMD), suggesting a translation ...
Andjus, Sara, Szachnowski, Ugo, Vogt, Nicolas, Gioftsidi, Stamatia, Hatin, Isabelle, Cornu, David, Papadopoulos, Chris, Lopes, Anne, Namy, Olivier, Wery, Maxime, Morillon, Antonin   +10 more
openaire   +3 more sources

Chimeric Zika Viruses Containing xrRNA1 Elements of Divergent Flaviviruses Retain the Ability to Replicate in Vertebrate and Mosquito Cells and Produce Subgenomic Flaviviral RNA

Viruses
Members of the Flaviviridae encode XRN1-resistant RNA structures (xrRNAs) within their 3′ untranslated regions (UTRs) that stall the cellular 5′→3′ exoribonuclease 1 (XRN1), resulting in the accumulation of subgenomic flavivirus RNAs (sfRNAs). Zika virus
Chandra S. Tangudu, Wichan Dankaona, Nile L. Stoltz, Tyler R. Simpkins, Bradley J. Blitvich   +4 more
doaj   +1 more source

XRN1 supplies free nucleotides to feed alphavirus replication

Abstract Several RNA viruses induce widespread degradation of cellular mRNAs upon infection; however, the biological significance and mechanistic details of this phenomenon remain unknown. Here, we make use of a model alphavirus, Sindbis virus (SINV), to fill this knowledge gap. We found that SINV triggers cellular RNA decay through the
Ruscica V, Iselin L, Hull R, Embarc-Buh A, Narayanan S, Palmalux N, Raut N, Gu Q, Chen H, Noerenberg M, de Laurent ZR, Joseph J, Noble M, Igreja C, Robertson DL, Hughes J, Mohammed S, Pelechano V, Davis I, Castello A.   +19 more
europepmc   +3 more sources

Both knocking-down and knocking-out of XRN1 had no effect on the accumulation of sfRNA.

, 2018
XRN1-knockdown (KD) cells were prepared as described in Materials and Methods. Knockdown efficiency was determined by Western blot using anti-XRN1 and anti-β-actin antibodies. The Rel.
Chih-Feng Tien (5563205), Yi-Shiuan Chen (5563202), Andrew Yueh (1514656), Ruey-Yi Chang (5563208), Yi-Hsin Fan (3585917)   +4 more
core   +1 more source

Characterisation of the in-vivo miRNA landscape in Drosophila ribonuclease mutants reveals Pacman-mediated regulation of the highly conserved let-7 cluster during apoptotic processes

Frontiers in Genetics
The control of gene expression is a fundamental process essential for correct development and to maintain homeostasis. Many post-transcriptional mechanisms exist to maintain the correct levels of each RNA transcript within the cell.
Elisa I. M. Bernard, Benjamin P. Towler, Benjamin P. Towler, Oliver M. Rogoyski, Sarah F. Newbury   +4 more
doaj   +1 more source

Xrn1 associates with eisosomes in post-diauxic cells.

, 2015
Cellular distribution of Xrn1-GFP (A, D, green in C, F) was compared with those of Sur7-mRFP (B, red in C) and Pil1-mRFP (E, red in F) in wild type post-diauxic cells (strains CRY1229 and CRY1187).
Tomas Grousl (382128), Miroslava Opekarová (172850), Jiri Hasek (382134), Jan Malinsky (172856), Vendula Stradalova (172840)   +4 more
core   +1 more source

Crystal structure of the 5′ → 3′ exoribonuclease Xrn1 [PDF]

Acta Crystallographica Section A Foundations of Crystallography, 2011
J. H. Chang, L. Tong
openaire   +1 more source

Mechanism of Xrn1 duplex RNA unwinding

RNA metabolism plays a central role in the regulation of gene expression, enabling cells to precisely control RNA abundance, quality, and function. In this context, RNA degradation is essential for maintaining cellular homeostasis, eliminating defective ...
Junhyuk Rhee
core  

ePRINT: exonuclease assisted mapping of protein-RNA interactions

Genome Biology
RNA-binding proteins (RBPs) regulate key aspects of RNA processing including alternative splicing, mRNA degradation and localization by physically binding RNA molecules.
Sophie Hawkins, Alexandre Mondaini, Seema C. Namboori, Grady G. Nguyen, Gene W. Yeo, Asif Javed, Akshay Bhinge   +6 more
doaj   +1 more source

Localization patterns of Xrn1 in three phases of aerobic growth.

, 2015
Cells expressing Xrn1-GFP (strain CRY1703) were observed 3 (A; log phase), 24 (B; diauxic shift), and 30 hours (C; post-diauxic shift) after the inoculation to complete synthetic medium supplemented with 2% glucose.
Tomas Grousl (382128), Miroslava Opekarová (172850), Jiri Hasek (382134), Jan Malinsky (172856), Vendula Stradalova (172840)   +4 more
core   +1 more source