Evidence that Xrn1 is in complex with Gcn1, and is required for full levels of eIF2α phosphorylation [PDF]
Biochemical JournalThe protein kinase Gcn2 and its effector protein Gcn1 are part of the general amino acid control signalling (GAAC) pathway best known in yeast for its function in maintaining amino acid homeostasis. Under amino acid limitation, Gcn2 becomes activated, subsequently increasing the levels of phosphorylated eIF2α (eIF2α-P).
Renuka Shanmugam +3 more
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Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. [PDF]
PLoS ONEThe budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. In the cytoplasm, apart from catalyzing 5'→3' decay onto non translated mRNAs, Xrn1 can
Antonio Jordán-Pla +7 more
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Comparison of Xrn1 and Rat1 5′ → 3′ exoribonucleases in budding yeast supports the specific role of Xrn1 in cotranslational mRNA decay [PDF]
YeastAbstractThe yeast Saccharomyces cerevisiae and most eukaryotes carry two 5′ → 3′ exoribonuclease paralogs. In yeast, they are called Xrn1, which shuttles between the nucleus and the cytoplasm, and executes major cytoplasmic messenger RNA (mRNA) decay, and Rat1, which carries a strong nuclear localization sequence (NLS) and localizes to the nucleus ...
José E. Pérez‐Ortín +11 more
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The zinc finger protein ZFP36L2 inhibits flavivirus infection via the 5′-3′ XRN1-mediated RNA decay pathway in the replication complexes [PDF]
Journal of Biomedical ScienceBackground The zinc finger protein 36-like (ZFP36L) family is a CCCH-type group consisting of RNA-binding proteins, i.e., ZFP36L1 and ZFP36L2, which regulate cellular mRNA through the RNA decay pathway. ZFP36L1 combats flavivirus infections through the 5′
Ren-Jye Lin +5 more
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Coupled 5′ Nucleotide Recognition and Processivity in Xrn1-Mediated mRNA Decay [PDF]
Molecular Cell, 2011 Messenger RNA decay plays a central role in the regulation and surveillance of eukaryotic gene expression. The conserved multidomain exoribonuclease Xrn1 targets cytoplasmic RNA substrates marked by a 5' monophosphate for processive 5'-to-3' degradation by an unknown mechanism. Here, we report the crystal structure of an Xrn1-substrate complex.
Jinek, Martin +2 more
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Correction: XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts. [PDF]
PLoS Pathogens, 2016 [This corrects the article DOI: 10.1371/journal.ppat.1005890.].
PLOS Pathogens Staff
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XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.
PLoS Pathogens, 2016 In eukaryotes, the degradation of cellular mRNAs is accomplished by Xrn1 and the cytoplasmic exosome. Because viral RNAs often lack canonical caps or poly-A tails, they can also be vulnerable to degradation by these host exonucleases.
Paul A Rowley +4 more
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Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences [PDF]
Nature Communications, 2016 Degradation of messenger RNA is a key regulatory step in controlling eukaryotic gene expression. Here the authors present xrFrag, a molecular tool to interrogate the extent and directionality of mRNA turnover by the detection of stabilized decay ...
Volker Boehm +3 more
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Xrn1/Pacman affects apoptosis and regulates expression of hid and reaper [PDF]
Biology Open, 2015 Programmed cell death, or apoptosis, is a highly conserved cellular process that is crucial for tissue homeostasis under normal development as well as environmental stress.
Joseph A. Waldron +8 more
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The roles of the exoribonucleases DIS3L2 and XRN1 in human disease [PDF]
Biochemical Society Transactions, 2016 RNA degradation is a vital post-transcriptional process which ensures that transcripts are maintained at the correct level within the cell. DIS3L2 and XRN1 are conserved exoribonucleases that are critical for the degradation of cytoplasmic RNAs. Although the molecular mechanisms of RNA degradation by DIS3L2 and XRN1 have been well studied, less is ...
Pashler, Amy L +3 more
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