Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. [PDF]
, 2016 Many gene expression factors contain repetitive phosphorylation sites for single kinases, but the functional significance is poorly understood. Here we present evidence for hyperphosphorylation as a mechanism allowing UPF1, the central factor in nonsense-
Durand, Sébastien +2 more
core +3 more sources
Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability
, 2023 Abstract Autophagy is a conserved process of cellular self-digestion that promotes survival during nutrient stress. In yeast, methionine starvation is sufficient to induce autophagy. One pathway of autophagy induction is governed by the SEACIT complex, which regulates TORC1 activity in response to amino acids through the Rag GTPases Gtr1 and Gtr2 ...
Madeline M. McGinnis +3 more
openaire +2 more sources
Unconventional miR-122 binding stabilizes the HCV genome by forming a trimolecular RNA structure. [PDF]
, 2013 MicroRNAs (miRNAs) typically downregulate protein expression from target mRNAs through limited base-pairing interactions between the 5' 'seed' region of the miRNA and the mRNA 3' untranslated region (3'UTR). In contrast to this established mode of action,
Doudna, Jennifer A, Mortimer, Stefanie A
core +1 more source
KSHV SOX mediated host shutoff: the molecular mechanism underlying mRNA transcript processing [PDF]
, 2017 Onset of the lytic phase in the KSHV life cycle is accompanied by the rapid, global degradation of host (and viral) mRNA transcripts in a process termed host shutoff. Key to this destruction is the virally encoded alkaline exonuclease SOX.
Bagnéris, C +7 more
core +6 more sources
Inhibition of type I interferon induction and signalling by mosquito-borne flaviviruses [PDF]
, 2017 The Flavivirus genus (Flaviviridae family) contains a number of important human pathogens, including dengue and Zika viruses, which have the potential to cause severe disease.
Clark, Jordan J. +3 more
core +1 more source
Endogenous RNAi pathway evolutionarily shapes the destiny of the antisense lncRNAs transcriptome
Life Science Alliance, 2019 A genome-wide comparative analysis of “cryptic” aslncRNAs decay in RNAi-capable and RNAi-deficient budding yeasts suggests an evolutionary contribution of RNAi in shaping the aslncRNAs transcriptome.
Ugo Szachnowski +4 more
doaj +1 more source
Communications Biology, 2023 Most functional eukaryotic mRNAs contain a 5′ 7-methylguanosine (m7G) cap. Although capping is essential for many biological processes including mRNA processing, export and translation, the fate of uncapped transcripts has not been studied extensively ...
Onofrio Zanin +6 more
doaj +1 more source
Engineered viral RNA decay intermediates to assess XRN1-mediated decay [PDF]
Methods, 2019 Both RNA synthesis and decay must be balanced within a cell to achieve proper gene expression. Additionally, modulation of RNA decay specifically offers the cell an opportunity to rapidly reshape the transcriptome in response to specific stimuli or cues.
Joseph, Russo +4 more
openaire +2 more sources
Wolbachia-mediated virus blocking in mosquito cells is dependent on XRN1-mediated viral RNA degradation and influenced by viral replication rate. [PDF]
PLoS Pathogens, 2018 Wolbachia is currently being developed as a novel tool to block the transmission of dengue viruses (DENV) by Aedes aegypti. A number of mechanisms have been proposed to explain the DENV-blocking phenotype in mosquitoes, including competition for fatty ...
Saijo Thomas +3 more
doaj +1 more source
The 5'-3' exoribonuclease pacman is required for epithelial sheet sealing in Drosophila and genetically interacts with the phosphatase puckered [PDF]
, 2008 Background information. Ribonucleases have been well studied in yeast and bacteria, but their biological significance to developmental processes in multicellular organisms is not well understood.
Browne, Cathy +7 more
core +2 more sources