Results 41 to 50 of about 7,632 (199)

Regulation of telomere metabolism by the RNA processing protein Xrn1 [PDF]

Nucleic Acids Research, 2017
Telomeric DNA consists of repetitive G-rich sequences that terminate with a 3΄-ended single stranded overhang (G-tail), which is important for telomere extension by telomerase. Several proteins, including the CST complex, are necessary to maintain telomere structure and length in both yeast and mammals.
Cesena, D, Cassani, C, Rizzo, E, Lisby, M, Bonetti, D, Longhese, M   +5 more
openaire   +4 more sources

Solid-phase XRN1 reactions for RNA cleavage: application in single-molecule sequencing [PDF]

Nucleic Acids Research, 2021
Abstract Modifications in RNA are numerous (∼170) and in higher numbers compared to DNA (∼5) making the ability to sequence an RNA molecule to identify these modifications highly tenuous using next generation sequencing (NGS). The ability to immobilize an exoribonuclease enzyme, such as XRN1, to a solid support while maintaining its ...
Athapattu, Uditha S., Amarasekara, Charuni A., Immel, Jacob R., Bloom, Steven, Barany, Francis, Nagel, Aaron C., Soper, Steven A.   +6 more
openaire   +3 more sources

Pseudoknot length modulates the folding, conformational dynamics, and robustness of Xrn1 resistance of flaviviral xrRNAs

Nature Communications, 2021
Exoribonuclease-resistant RNAs (xrRNAs) are RNA elements that block the exoribonucleolytic degradation of RNA. Here the authors show how a long-range pseudoknot length modulates the Mg2+-dependence of flaviviral xrRNA’s folding, conformational dynamics ...
Xiaolin Niu, Ruirui Sun, Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen, Xianyang Fang   +6 more
doaj   +1 more source

XRN1 stalling in the 5' UTR of Hepatitis C virus and Bovine Viral Diarrhea virus is associated with dysregulated host mRNA stability. [PDF]

PLoS Pathogens, 2015
We demonstrate that both Hepatitis C virus (HCV) and Bovine Viral Diarrhea virus (BVDV) contain regions in their 5' UTRs that stall and repress the enzymatic activity of the cellular 5'-3' exoribonuclease XRN1, resulting in dramatic changes in the ...
Stephanie L Moon, Jeffrey G Blackinton, John R Anderson, Mary K Dozier, Benjamin J T Dodd, Jack D Keene, Carol J Wilusz, Shelton S Bradrick, Jeffrey Wilusz   +8 more
doaj   +1 more source

Dcp2 C-terminal cis-binding elements control selective targeting of the decapping enzyme by forming distinct decapping complexes

eLife, 2022
A single Dcp1–Dcp2 decapping enzyme targets diverse classes of yeast mRNAs for decapping-dependent 5′ to 3′ decay, but the molecular mechanisms controlling mRNA selectivity by the enzyme remain elusive.
Feng He, Chan Wu, Allan Jacobson
doaj   +1 more source

Xrn1-resistant RNA structures are well-conserved within the genus flavivirus [PDF]

RNA Biology, 2020
Subgenomic RNAs are produced by several RNA viruses through incomplete degradation of their genomic RNA by the exoribonuclease Xrn1, and have been shown to be essential for viral growth and pathogenicity. Within the flavivirus genus of the Flaviviridae family, two distinct classes of Xrn1-resistant RNA motifs have been proposed; one for mosquito-borne ...
Ivar W. Dilweg, Assia Bouabda, Tim Dalebout, Alexander P. Gultyaev, Peter J. Bredenbeek, R. C. L. Olsthoorn   +5 more
openaire   +4 more sources

Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. [PDF]

PLoS Genetics, 2015
Retrotransposition of the budding yeast long terminal repeat retrotransposon Ty3 is activated during mating. In this study, proteins that associate with Ty3 Gag3 capsid protein during virus-like particle (VLP) assembly were identified by mass ...
Virginia Bilanchone, Kristina Clemens, Robyn Kaake, Anthony R Dawson, Dina Matheos, Kunio Nagashima, Parth Sitlani, Kurt Patterson, Ivan Chang, Lan Huang, Suzanne Sandmeyer   +10 more
doaj   +1 more source

Processing of RNA Containing 8-Oxo-7,8-Dihydroguanosine (8-oxoG) by the Exoribonuclease Xrn-1

Frontiers in Molecular Biosciences, 2021
Understanding how oxidatively damaged RNA is handled intracellularly is of relevance due to the link between oxidized RNA and the progression/development of some diseases as well as aging.
Cheyenne N. Phillips, Shawn Schowe, Conner J. Langeberg, Namoos Siddique, Erich G. Chapman, Marino J. E. Resendiz   +5 more
doaj   +1 more source

The 5'-3' exoribonuclease Pacman (Xrn1) regulates expression of the heat shock protein Hsp67Bc and the microRNA miR-277-3p in Drosophila wing imaginal discs [PDF]

, 2013
Pacman/Xrn1 is a highly conserved exoribonuclease known to play a critical role in gene regulatory events such as control of mRNA stability, RNA interference and regulation via miRNAs.
Bate M, Carballo E, Christopher I Jones, Dominic P Grima, Hannah N Parker, Joseph A Waldron, Sarah F Newbury, Sue Jones   +7 more
core   +1 more source

Dynamic “Cap”‐abilities of P‐bodies and the XRN1‐EDC4 axis

The EMBO Journal, 2023
RNA turnover regulates the quality and quantity of cellular gene expression through a coordinated cavalcade of enzymes, factors, and phase transitions. In this issue, Brothers et al reveal the importance of balanced communication between the Xrn1 exonuclease and the EDC4 decapping factor to coordinate P‐body dynamics and maintain cellular fitness.
Lauren E Malsick, Jeffrey Wilusz
openaire   +2 more sources