Results 51 to 60 of about 7,264 (130)
Advances in large DNA fragment assembly for microbial cell factory engineering
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang +5 more
wiley +1 more source
Background Xylose reductase (XR) and xylitol dehydrogenase (XDH) from Pichia stipitis are the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains engineered for xylose utilization.
Hahn-Hägerdal Bärbel +2 more
doaj +1 more source
This review integrates chemical and biological xylitol production from upstream to downstream, uniquely combining experimental and theoretical insights to reveal mechanisms, bottlenecks, and future directions for sustainable lignocellulosic xylitol production.
Tanongsak Sukkasem +2 more
wiley +1 more source
ABSTRACT Yeast sugar transporters have highly evolved for preferential glucose transport, a significant roadblock for utilizing non‐glucose sugars in renewable feedstocks such as lignocellulosic biomass. To enable simultaneous transport of multiple sugars, native hexose transporters were replaced by SWEET7p from Arabidopsis thaliana in engineered ...
Nurzhan Kuanyshev +6 more
wiley +1 more source
Metabolic Engineering of Escherichia coli for Xylitol Production
Xylitol is a sugar–alcohol compound with broad applications in fields such as the food, dental, and pharmaceutical sectors. Although xylitol biosynthesis has gained attention, the current strategy for industrial xylitol production majorly relies on the ...
Jiapeng Li +5 more
doaj +1 more source
To address the GWAS data scarcity in Pyropia haitanensis, 280 double‐haploid strains were phenotyped for 11 agronomic traits. GWAS analysis identified 454 candidate genes clustered on chromosome 4. Two molecular markers were validated: RPN5 (C/G SNP at Chr4:4,319,644) for thallus length, and DWF1 (T/G SNP at Chr4:4,485,590) for maturity days ...
Yan Xu +7 more
wiley +1 more source
Construction of a CO2‐Fixing Compartment Using a Shape‐Transforming DNA Scaffold
A shape‐transforming DNA scaffold encapsulates RuBisCO within a controllable, self‐contacting compartment that mimics the carboxysome. The scaffold switches between open and closed states, enabling direct comparison of identical enzyme populations in different microenvironments.
Hui Yang +6 more
wiley +1 more source
Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals.
Suryang Kwak, Yong-Su Jin
doaj +1 more source
Comparative assessment of fermentative capacity of different xylose-consuming yeasts
Background Understanding the effects of oxygen levels on yeast xylose metabolism would benefit ethanol production. In this work, xylose fermentative capacity of Scheffersomyces stipitis, Spathaspora passalidarum, Spathaspora arborariae and Candida tenuis
Henrique César Teixeira Veras +2 more
doaj +1 more source
This study employed an ultrasonic‐assisted extraction (UAE) method combined with column chromatography purification to obtain two homogeneous polysaccharides (CVP‐I and CVP‐II) from Conioselinum vaginatum, which exhibited distinct physicochemical properties and structural characteristics, and exerted anti‐inflammatory effects by inhibiting the ...
Conghui Ren +6 more
wiley +1 more source

