Results 161 to 170 of about 257,444 (202)

Affinity and Stability Analysis of Yeast Displayed Proteins

2022
Yeast surface display is a powerful protein engineering technology that is extensively used to improve various properties of proteins, including affinity, specificity, and stability or even to add novel functions (usually ligand binding). Apart from its robustness and versatility as an engineering tool, yeast display offers a further critical advantage:
Charlotte U, Zajc, Magdalena, Teufl, Michael W, Traxlmayr   +2 more
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Protein Engineering and Selection Using Yeast Surface Display

2015
Yeast surface display is a powerful technology for engineering a broad range of protein scaffolds. This protocol describes the process for de novo isolation of protein binders from large combinatorial libraries displayed on yeast by using magnetic bead separation followed by flow cytometry-based selection.
ANGELINI, Alessandro, Chen TF, De Picciotto S, Yang NJ, Tzeng A, Santos MS, Van Deventer JA, Traxlmayr MW, Wittrup KD   +8 more
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Yeast surface display for screening combinatorial polypeptide libraries

Nature Biotechnology, 1997
Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity.
E T, Boder, K D, Wittrup
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Ligand Engineering Using Yeast Surface Display

2014
The genotype-phenotype linkage provided by display technologies enables efficient synthesis, analysis, and selection of combinatorial protein libraries. This approach tremendously expands the protein sequence space that can be efficiently evaluated for a selectable function.
openaire   +2 more sources

Differential display analysis of gene expression in yeast

Cellular and Molecular Life Sciences (CMLS), 2002
RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model for studying basic functions of the eukaryotic genome ...
A V, Ivanova, S V, Ivanov
openaire   +2 more sources

Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display

2022
Yeast surface display (YSD) has been extensively used for protein design, engineering, and directed evolution in the past two decades. Here, we describe methods for directed evolution of tissue inhibitors of metalloproteinase (TIMP), the natural inhibitors of matrix metalloproteinases (MMPs), through design and generation of a combinatorial library of ...
Mari R, Toumaian, Maryam, Raeeszadeh-Sarmazdeh   +1 more
openaire   +2 more sources

Selection of Antibody Fragments by Yeast Display

2012
The critical need for renewable, high-quality affinity reagents in biological research, as well as for diagnostic and therapeutic applications, has required the development of new platforms of discovery. Yeast display is one of the main methods of in vitro display technology with phage display. Yeast display has been chosen by numerous groups to refine
openaire   +3 more sources

Yeast surface display technology: Mechanisms, applications, and perspectives

Biotechnology Advances
Microbial cell surface display technology, which relies on genetically fusing heterologous target proteins to the cell wall through fusion with cell wall anchor proteins, has emerged as a promising and powerful method with diverse applications in biotechnology and biomedicine.
Yibo, Li, Xu, Wang, Ning-Yi, Zhou, Junmei, Ding   +3 more
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Flow Cytometric Screening of Yeast Surface Display Libraries

2004
A method to screen and isolate antigen specific clones from a library of single-chain antibodies expressed on the surface of yeast cells is presented. Two rounds of magnetic bead enrichment before flow cytometric sorting enables one to screen libraries of far greater diversity than can be screened by just flow cytometry. The strength of flow cytometric
Michael, Feldhaus, Robert, Siegel
openaire   +2 more sources

Isolating and engineering human antibodies using yeast surface display

Nature Protocols, 2006
This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display. Single-chain antibody fragments (scFvs) are first isolated from an existing nonimmune human library displayed on the yeast surface using magnetic-activated cell sorting selection followed by selection
Ginger, Chao, Wai L, Lau, Benjamin J, Hackel, Stephen L, Sazinsky, Shaun M, Lippow, K Dane, Wittrup   +5 more
openaire   +2 more sources