Results 31 to 40 of about 257,444 (202)

Yeast surface display-based microfluidic immunoassay

Sensors and Actuators B: Chemical, 2012
In this paper, we present a new microfluidic immunoassay platform, which is based on the synergistic combination of the yeast surface display (YSD) technique and the microfluidic technology. Utilizing the YSD technique, antigens specific to the target antibody are displayed on the surface of engineered yeast cells with intracellular fluorescent ...
Wang, Jing, Cheng, Danhui, Chan, Kwok Lun, Luo, Xiaoteng, Wu, Hongkai, Hsing, I-Ming   +5 more
openaire   +3 more sources

Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes [PDF]

, 2006
ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin remodelling activities in eukaryotes from yeast to man. Many of these complexes have been found to reposition nucleosomes, but with different directionalities.
Alen, Beard, Becker, Brehm, Bruno, Bruno, Davey, Dillingham, Drew, Eberharter, Elfring, Fazzio, Flaus, Flaus, Flaus, Hamiche, Kagalwala, Kang, Kassabov, Langst, Lorch, Lowary, Luger, Lusser, Meersseman, Mizuguchi, Morillon, Rigaut, Schnitzler, Tran, Tsukiyama, Tsukiyama, Vary, Zofall   +33 more
core   +4 more sources

Yeast Display of Engineered Antibody Domains [PDF]

, 2012
Yeast display is an efficient technology for selection of antibodies and other proteins with high affinity and thermal stability. Here, we describe a method for affinity maturation of engineered antibody domains (eAds) using yeast display. EAd yeast libraries of relatively large size (∼10⁹) were generated and subjected to alternating rounds of magnetic-
Qi, Zhao, Zhongyu, Zhu, Dimiter S, Dimitrov   +2 more
openaire   +2 more sources

Principles of meiotic chromosome assembly revealed in S. cerevisiae [PDF]

, 2019
During meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear.
A Goloborodko, A Hayashi, A Lengronne, A Storlazzi, A Tedeschi, AL Sanborn, AV Smith, B Bonev, D Thacker, D Zickler, E Alipour, E Crane, E Trelles-Sticken, EF Glynn, EP Nora, EP Nora, F Klein, G Fudenberg, G Fudenberg, G Fudenberg, G Wutz, GA Busslinger, H Jin, H Muller, H Tjong, H Wong, J Dekker, J Dekker, J Gassler, J Li, JA Carballo, JF Marko, JHI Haarhuis, JM Belton, JR Dixon, K Challa, K Nasmyth, KG Alavattam, L Lazar-Stefanita, L Patel, L Xu, M Ito, M Merkenschlager, M Sym, M Sym, MN Conrad, N Naumova, N Servant, NJ Petela, NM Hollingsworth, O Crawley, P Eastman, P Eastman, P Kerpedjiev, PB Møens, Q Jin, S Heinz, S Maleki, S Panizza, SA Schalbetter, SSP Rao, SSP Rao, T Mizuguchi, T Sakuno, TS Hsieh, VV Subramanian, W Schwarzer, Y Blat, Y Kakui, Y Wang   +69 more
core   +2 more sources

The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance [PDF]

, 2011
The multiprotein complexes that receive and transmit axon pathfinding cues during development are essential to circuit generation. Here, we identify and characterize the Drosophila sterile α-motif (SAM) domain-containing protein Caskin, which shares ...
Broihier, Heather T, DiAntonio, Aaron, Liu, Nan, Weng, Yi-Lan   +3 more
core   +2 more sources

Soluble T Cell Receptor Vβ Domains Engineered for High-Affinity Binding to Staphylococcal or Streptococcal Superantigens

Toxins, 2014
Staphylococcus aureus and group A Streptococcus secrete a collection of toxins called superantigens (SAgs), so-called because they stimulate a large fraction of an individual’s T cells.
Preeti Sharma, Ningyan Wang, David M. Kranz   +2 more
doaj   +1 more source

Construction of Yeast Surface-Displayed cDNA Libraries [PDF]

, 2011
Using yeast display, heterologous protein fragments can be efficiently displayed at high copy levels on the Saccharomyces cerevisiae cell wall. Yeast display can be used to screen large expressed protein libraries for proteins or protein fragments with specific binding properties.
Scott, Bidlingmaier, Bin, Liu
openaire   +2 more sources

De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against Virions

Journal of Biological Engineering, 2019
Background The promise of biopharmaceuticals comprising one or more binding domains motivates the development of novel methods for de novo isolation and affinity maturation of virion-binding domains.
Pete Heinzelman, Alyssa Low, Rudo Simeon, Gus A. Wright, Zhilei Chen   +4 more
doaj   +1 more source

The evolutionary rewiring of ubiquitination targets has reprogrammed the regulation of carbon assimilation in the pathogenic yeast Candida albicans [PDF]

, 2012
Date of Acceptance: 13/11/2012 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in ...
Ashe, Atir-Lande, Barelle, Barford, Beckwith, Brown, Buchalter, Calderone, Carlson, Cline, Crabtree, de Nobel, Ene, Enjalbert, Enjalbert, Enjalbert, Entian, Fradin, Futcher, Gancedo, Gancedo, Gancedo, Garreau, Gounalaki, Gygi, Görner, Horak, Hämmerle, Ihmels, Imbeaud, Jacob, Janke, Jiang, Johnston, Kaniak, Knop, Kusch, Lavoie, Leach, Leach, Leng, Li, Lorenz, Lorenz, López-Boado, Martchenko, Mercado, Mercado, Mercado, Merico, Nantel, Niimi, Odds, Ozcan, Piekarska, Radivojac, Ramírez, Regelmann, Rodaki, Roig, Roig, Rolland, Sabina, Scheffler, Schüle, Sherman, Smith, Stanhill, Thevelein, Thewes, Trunk, Tsong, Ueno, Usaite, Walker, Yin, Yin, Yin, Yin   +78 more
core   +1 more source

A novel one-step approach for the construction of yeast surface display Fab antibody libraries

Microbial Cell Factories, 2018
Background Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as ...
Simon Rosowski, Stefan Becker, Lars Toleikis, Bernhard Valldorf, Julius Grzeschik, Deniz Demir, Iris Willenbücher, Ramona Gaa, Harald Kolmar, Stefan Zielonka, Simon Krah   +10 more
doaj   +1 more source