Results 101 to 110 of about 21,615 (216)

Gelatin zymography assay on CatK activity.

open access: yes, 2015
a) Left lanes: Human recombinant CatK as positive control and right lanes: CatK extracted from RAW264.7 cells (RAW OC) treated with 0,5,10,100,1000 nM of CatK inhibitors (CKI-8 and CKI-13); b) CatK (OC) extracted from osteoclasts differentiated from bone
Zhong-Yuan Ren (769126)   +6 more
core   +1 more source

Development of "cell in situ collagen zymography" method for determining local activities of collagenases

open access: yes, 2022
Collagenases (MMP-1,-8,-13) are enzymes belonging to the family of matrix metalloproteinases and play significant roles in tumor invasion and metastasis.
Savaş, Ege Gökçe   +2 more
core   +1 more source

FLOW INJECTION ZYMOGRAPHY FOR THE ON-LINE DETECTION OF ENZYMEACTIVITY [PDF]

open access: yes
new method - Flow Injection Zymography - has been developed for the detection of enzymeactivity during purification procedures usingflow injection analysis (FIA). Four oxidases and a lipase were used as model enzymes.
Heine-Dobbernack, Elke   +3 more
core   +1 more source

Spatial patterns of extracellular enzymes: Combining X-ray computed micro-tomography and 2D zymography

open access: yes, 2019
Linking the distribution of enzyme activity to the size and properties of soil pores is a necessary prerequisite for mechanistic understanding of soil biochemical processes.
Guber A.K.   +5 more
core   +2 more sources

Combined in Situ Zymography, Immunofluorescence, and Staining of Iron Oxide Particles in Paraffin-Embedded, Zinc-Fixed Tissue Sections

open access: yesMolecular Imaging, 2012
Superparamagnetic iron oxide particles are used as potent contrast agents in magnetic resonance imaging. In histology, these particles are frequently visualized by Prussian blue iron staining of aldehyde-fixed, paraffin-embedded tissues.
Eyk Schellenberger   +6 more
doaj   +1 more source

A screening method for β-glucan hydrolase employing Trypan Blue-coupled β-glucan agar plate and β-glucan zymography

open access: yes, 2012
A new screening method for β-(1,3-1,6) glucan hydrolase was developed using a pure β-glucan from Aureobaisidum pullulans by zymography and an LB-agar plate. Paenibacillus sp.
D O Kang   +5 more
core   +1 more source

Silver-stained fibrin zymography: separation of proteases and activity detection using a single substrate-containing gel

open access: yes, 2011
A new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed.
Keug Hyun Ahn   +19 more
core   +1 more source

Gelatinolytic activity in dentin upon adhesive treatment

open access: yesScientific Reports
In this multi-parameter study, the effect of diverse factors related to adhesive application on the activation of host-derived gelatinases was investigated by gelatin zymography, in-situ zymography, fluorogenic DQ-gelatin assay and micro-tensile bond ...
Xin Li   +6 more
doaj   +1 more source

MMP-2 and -9 Activities Revealed Using Zymography.

open access: yes, 2015
MMP activities in the ischemic hemisphere were measured using gelatin zymography. The MSC 1-hour group showed higher MMP-2 activity than did the control group (P = 0.028).
Ji Hoe Heo (147873)   +11 more
core   +1 more source

ASSESSMENT OF INDIRECT HEMAGGLUTINATION AND ZYMOGRAPHY PROCEDURES IN EVALUATION OF GELATINASE A IN PATIENTS WITH BENIGN AND MALIGNANT PROSTATE HYPERPLASIA

open access: yesIranian Journal of Allergy, Asthma and Immunology, 2003
There are increasing data on novel tumor markers such as gelatinase A, which play a key role in tissue invasion and metastasis. Since prostate cancer is one of the common malignancies, we designed a simple and applicable Indi¬rect Hemagglutination (IHA ...
Nastaran Aalizadeh   +3 more
doaj  

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