Results 91 to 100 of about 21,615 (216)
By changing the buffer composition from phosphate to acetate (in both cases, the pH value was 6.0), the sensitivity of the estimation of peroxidase activity in polyacrylamide gels was improved from 4 µg/well to 0.4 µg/well.
Gheorghița MENGHIU +2 more
doaj +1 more source
2D zymography reveals enzymatic isoforms of gelatinases of brain tissues from ZO rats.
(A) Gelatinase activity from the brain tissues of the Zucker lean and obese rats was visualized using 1D gelatin zymography. ProMMP-9, act.MMP-9 and proMMP-2 were identified as bright bands.
Jennifer M. Wesley (720661) +9 more
core +1 more source
Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity [PDF]
Lipases are esterases that occur widely in nature, yet those with commercial relevance are exclusively from microbial origin. Glycerol and long-chain fatty acids are the products after hydrolysis of esters bonds in saponifiable lipids catalyzed by ...
Tapizquent, María +6 more
core +1 more source
Gelatin zymography for MMP-2 and -9.
(A) Representative image of gelatin zymography for MMP-2 and -9 of the corneas 14 days after alkali burns, treated with intraperitoneal injection of PBS, minocycline (30 mg/kg b.i.d) and minocycline (60 mg/kg b.i.d).
Bin-wu Lin (314370) +5 more
core +1 more source
Soil membrane zymography enables 2D mapping of enzyme activities on the surface of soil samples. The method is based on diffusion of components of enzymatically-mediated reactions to/from membrane, and, thus, reflects the distribution of enzyme ...
Guber A. +6 more
core +1 more source
Zymography of recombinant MMP-2 protein and excised HT1080 tumor homogenates.
Zymography of recombinant MMP-2 protein and excised HT1080 tumor homogenates.
Takashi Temma (551579) +8 more
core +1 more source
Ex vivo Follicle Rupture and in situ Zymography in Drosophila
Ovulation, the process of releasing a mature oocyte from the ovary, is crucial for animal reproduction. In order for the process of ovulation to occur, a follicle must be fully matured and signaled to rupture from the ovary.
Elizabeth Knapp +2 more
doaj +1 more source
Abstracts submitted to the ‘EACR 2026 Congress: Innovative Cancer Science’, from 08–11 June 2026 and accepted by the Congress Organising Committee are published in this Supplement of Molecular Oncology, an affiliated journal of the European Association for Cancer Research (EACR).
wiley +1 more source
Determination of Bacterial Gelatinase Production By Zymography
Streptococcus pyogenes has been shown to produce an extracellular cysteine protease which degrades gelatin, fibronectin, collagen, and other extracellular matrix (ECM) proteins.
Barnhart, Allen
core
Calibration of 2-D soil zymography for correct analysis of enzyme distribution
Soil zymography is a new technique developed to visualize two-dimensional distributions of enzyme activities. The method consists of incubating a membrane saturated with an enzyme-specific fluorogenic substrate on a surface of the soil sample, followed ...
Guber A.K. +4 more
core +1 more source

