Results 71 to 80 of about 21,615 (216)

Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors

BioTechniques, 2005
The balance between matrix metalloproteinases (MMPs) and their inhibitors, the tissue inhibitors of metalloproteinases (TIMPs), is largely responsible for the remodeling of tissues.
Patricia A.M. Snoek-van Beurden, Johannes W. Von den Hoff   +1 more
doaj   +1 more source

Adaptor protein CIN85 potentiates the motility of osteosarcoma cells via the Akt/mTOR and MMP2‐COL3A1 axis

Molecular Oncology, Volume 20, Issue 8, Page 2085-2101, August 2026.
CIN85 is highly expressed in osteosarcoma, particularly in metastatic lesions. Its overexpression increases cell migration and Matrigel invasion, while silencing CIN85 suppresses these behaviors. Transcriptome analysis shows that CIN85 regulates MMP2, COL3A1, and Akt/mTOR signaling. Targeting these pathways reverses CIN85‐induced motility, highlighting
Iryna Horak, Iva Staniczková Zambo, Matěj Přikryl, Peter Múdry, Danica Zapletalová, Jarmila Navrátilová, Jiří Navrátil, Jan Šmarda, Liudmyla Drobot, Petr Beneš, Lucia Knopfová   +10 more
wiley   +1 more source

Gelatin zymography of VGS supernatants and controls.

, 2018
Each concentration-normalized sample was subjected to zymography to detect protease activity. Zones of clearing indicate protease activity. Molecular mass standards in kDa are indicated to the left of the gels. Two independent experiments were performed,
Mary E. Marquart (399869), Regina C. Galloway (6112058), Lisa M. Stempak (6112061), Angela H. Benton (6112055)   +3 more
core   +1 more source

Integrated Network Analysis Suggests an miR‐21/MMP/VEGF‐Associated Regulatory Axis in Gastric Cancer

Cancer Reports, Volume 9, Issue 8, August 2026.
ABSTRACT Background, Aims Gastric cancer (GC) pathogenesis involves complex molecular interactions that remain incompletely understood at a biological systems level. While microRNA‐21 (miR‐21), matrix metalloproteinases (MMPs), and vascular endothelial growth factor (VEGF) have been individually implicated in GC, their integrated role as a coordinated ...
Fatemeh Babajani, Hadi Mozafari
wiley   +1 more source

Aerobic Endurance Training Modulates Cardiac Renin‐Angiotensin System Peptides and Matrix Metalloproteinase Activity During Chronic Stress

Journal of Cellular and Molecular Medicine, Volume 30, Issue 15, August 2026.
ABSTRACT To investigate the involvement of metalloproteinases (MMPs) in the context of endurance training and their interactions with renin‐angiotensin signalling (RAS) in stress‐induced pathophysiological responses. Trained rats were submitted to 8‐weeks of endurance treadmill training with chronic mild stress (CMS), TS rats, and without CMS (T).
Vinicius Guzzoni, Fabiana Gomes Ferreira, Lívia Bruni de Souza, Rebecca Salomão, Nicholas Kesler, Rodrigo Yokota, Nilsa Regina Damaceno‐Rodrigues, Elia Garcia Caldini, Fernanda Klein Marcondes, Rita de Cássia Marqueti, Steven T. Haller, David J. Kennedy, Lauren G. Koch, Dulce Elena Casarini, Tatiana Sousa Cunha   +14 more
wiley   +1 more source

Time-lapse approach to correct deficiencies of 2D soil zymography

Membrane zymography is commonly used in rhizosphere ecology to map enzyme activities in intact soil samples and plant roots. The method consists of incubating a membrane saturated with an enzyme-specific fluorogenic substrate on the soil/root surface ...
Juyal A., Guber A., Kuzyakov Y., Blagodatskaya E., Kravchenko A., Razavi B.S.   +5 more
core   +1 more source

Detection of rTs-Pt nuclease activity with SDS-PAGE zymography.

, 2014
(A) SDS-PAGE zymography analysis of rTs-Pt, after staining with ethidium bromide and exposure to UV light. (B) The same gel was stained with Coomassie Brilliant blue after zymography.
Mingyuan Liu (142151), Xuelin Wang (527015), Xue Bai (129175), Ying Zhao (16546), Chengshui Liao (527013), Qingsong Sun (622921), Pascal Boireau (622923), Xidong Liu (622922), Pan Liu (527011), Bin Tang (56654), Xiuping Wu (527012), Xiaolei Liu (104141), Feng Wang (44414), He Gao (86117), Tingting Li (10553)   +14 more
core   +1 more source

Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography [PDF]

We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the detection and characterization of proteases and protease inhibitors.
4073, 4074, 4075, Okamoto, Eishiro, 4076, Hoshino, Takashi, 4077, Taniguchi, Masayuki, 4078, Saitoh, Eiichi, Hayakawa, Yoshimi, 4079, Isemura, Satoko, Ohtsubo, Sadami, 4071, 4072, Sato, Ritsuko, Yamamoto, Shinya   +17 more
core   +1 more source

An Inexpensive Staining Alternative for Gelatin Zymography Gels

Methods and Protocols, 2019
Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel.
Christian Wechselberger, Christian Doppler, David Bernhard   +2 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, Volume 16, Issue 7, Page 1414-1427, July 2026.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source