Results 51 to 60 of about 14,446 (183)

Detection and Estimation of Active Proteinases in Biological Samples: An Optimized Protocol

Frontiers in Bioscience-Landmark
Background: Matrix metalloproteinases (MMPs) are enzymes that degrade extracellular matrix (ECM) proteins and activate cytokines and chemokines, playing a critical role in tissue remodeling.
Hebah Almarshood, Yu-Ting Zheng, Robert E. Brainard, Suresh C. Tyagi, Daniela Terson de Paleville, Michael N. Sekula, Utpal Sen   +6 more
doaj   +1 more source

Interactions of Antibody Drug Conjugate Anti‐Tubulin and Topoisomerase I Inhibitor Payloads with Radiotherapy to Potentiate Immunotherapy

Advanced Science, Volume 13, Issue 35, 24 June 2026.
Antibody drug conjugates deliver their cytotoxic anti‐tubulin or topoisomerase I inhibitor payloads to tumors through cancer cell receptor targeting. The released drug payloads induce cellular changes that interact with radiotherapy resulting in radiosensitization that improves cancer cell kill and stimulates anti‐tumor immune responses.
Jacqueline Lesperance, Bryan S. Yung, Michael M. Allevato, Marcus M Cheng, Maria F. Camargo, Robert Saddawi‐Konefka, Kanika Dhawan, Ashwyn K. Sharma, Mahsa Mortaja, Sophie Bice, Daniel J. Scanderbeg, Diego Alvarado, Jyoti Mayadev, Ramez N. Eskander, Stephen R. Adams, Pippa F. Cosper, J. Silvio Gutkind, Sunil J. Advani   +17 more
wiley   +1 more source

Vitamins as potent cancer treatments through regulating the activity and expression of MMP‐2 and MMP‐9

Bulletin of the Korean Chemical Society, Volume 47, Issue 6, Page 745-755, June 2026.
Vitamins could regulate both MMP‐2 and MMP‐9 expression and activity through transcriptional and MAPK signaling pathway modulation. This study highlights the potential of vitamins as low‐toxicity therapeutic or adjunct agents for targeting MMP‐mediated cancer progression. Abstract Matrix metalloproteinases (MMP‐2 and MMP‐9) are key regulators of cancer
Yen Thi Nguyen, Eungchan Kim, Ha Vy Thi Vo, Song Ja Kim, Namdoo Kim, Hyuck Jin Lee   +5 more
wiley   +1 more source

An Inexpensive Staining Alternative for Gelatin Zymography Gels

Methods and Protocols, 2019
Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel.
Christian Wechselberger, Christian Doppler, David Bernhard   +2 more
doaj   +1 more source

An improved collagen zymography approach for evaluating the collagenases MMP-1, MMP-8, and MMP-13

BioTechniques, 2017
Collagen zymography is an SDS-PAGE–based method for detecting both the proenzyme and active forms of collagenases. Although collagen zymography is used for assessment of the matrix metalloproteinases MMP-1 and MMP-13, it can be difficult to detect these ...
Seniz Inanc, Didem Keles, Gulgun Oktay
doaj   +1 more source

Milk‐Clotting Protease From Bacillus stercoris NCCP‐3139: A Potential Microbial Rennet for Cheese Production

Food Science &Nutrition, Volume 14, Issue 5, May 2026.
A milk‐clotting protease from Bacillus stercoris NCCP‐3139 was isolated from soil and purified via ammonium sulfate precipitation and sequential chromatographic techniques, yielding a homogeneous enzyme confirmed by SDS–PAGE. The protease exhibited high catalytic selectivity, with a marked increase in milk‐clotting‐to‐proteolytic activity ratio (50–550)
Muhammad Sibtain, Ashraf Ahmed Qurtam, Sadaf Javaria, Ghulam Murtaza, Mohammed Al‐zharani, Noor‐ul Ain, Sadia Chaman, Fahd A. Nasr, Ali Zaman   +8 more
wiley   +1 more source

Bacterial Extracellular Vesicles: New Hype or Hope to Explain Reproductive Host–Microbiota Interactions

Journal of Extracellular Vesicles, Volume 15, Issue 5, May 2026.
ABSTRACT Rapid advances in microbiome research are transforming our understanding of human health and disease, with growing focus on the female reproductive tract as a critical but understudied niche. Evidence for a local microbiome largely derives from bacterial nucleic acid detection; however, the biological relevance of these signals remains debated,
Hannah Wein, Paula Iglesias‐Moreno, Apostol Apostolov, Andres Salumets, Damián O. Muzzio, Alberto Sola‐Leyva   +5 more
wiley   +1 more source

Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy

Pesquisa Veterinária Brasileira
: Some studies have shown the role played by matrix metalloproteinases and their inhibitors in doxorubicin cardiotoxicity. In this study, we sought to investigate how plasma and myocardial MMP 2 and 9 perform in rabbits with doxorubicin-induced ...
Sheila S.S. Nogueira, Marlos G. Sousa, Fabio N. Gava, Fernando A. Rosa, Guilherme D. Melo, Gustavo Dittrich, Gisele F. Machado, Aparecido A. Camacho   +7 more
doaj   +1 more source

Endolysin Expression, Purification and Activity Determination by Zymography

Bio-Protocol, 2014
Endolysins are peptidoglycan-degrading (muralytic) enzymes produced by many bacteriophages for cell lysis of the host bacterium. The enzymatic activity of muralytic enzymes can be assayed qualitatively using a zymogram containing incorporated ...
Alexander Westbye, Paul Fogg, John Beatty   +2 more
doaj   +1 more source

AP‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways

Journal of Cellular and Molecular Medicine, Volume 30, Issue 9, May 2026.
ABSTRACT Numerous cellular functions, such as apoptosis, proliferation, differentiation, survival, transformation and cell migration, are regulated by a crucial transcription factor called activator protein 1 (AP‐1). Growing evidence indicates that AP‐1 is involved in severe conditions like fibrosis, cancer, and organ damage, as well as inflammatory ...
Maryam Mahjoubin‐Tehran, Samaneh Rezaei, Vasily N. Sukhorukov, Amin Jalili, Prashant Kesharwani, Amirhossein Sahebkar   +5 more
wiley   +1 more source