Results 31 to 40 of about 14,446 (183)
Gelatin Zymography of Major Proteases in Exosomes of Leishmania major Promastigotes
Iranian Journal of Parasitology, 2023 Background: Enzymatic digestion of extra cellular matrix proteins by proteinases of Leishmania promastigotes is a complex process. Hence, studies on functional proteomics of these enzymes can help select these enzymes as possible vaccine candidates or
Akram Azambakhtiar +4 more
doaj +1 more source
Design and Synthesis of Peptide‐Polyester Conjugates for Cell‐Mediated Scaffold Degradation
Advanced Healthcare Materials, EarlyView.This work describes polycaprolactone (PCL)‐based biomaterials engineered to degrade in response to cell‐secreted proteases. A fast‐degrading peptide (Fast) sequence is integrated into a PCL conjugate backbone to produce a biomaterial that is selectively degraded by multiple cell types compared to its scrambled control (ScrFast).
Korina Vida G. Sinad +7 more
wiley +1 more source
Microbiology Indonesia, 2011 Superoxide dismutase (SOD) has therapeutic importance because of its antioxidant activity and protects cells from reactive oxygen species attack. This research was intended to screen bacteria isolated from Indonesia for producing novel SODs and to ...
ANA INDRAYATI +3 more
doaj +1 more source
Animal Models and Experimental Medicine, EarlyView.Age‐related macular degeneration (AMD) involves lipid dysregulation and complement overactivation. Here, we characterize a double‐knockout ApoE−/−/Cfh−/− mouse as a model of early–intermediate retinal degeneration. These mice exhibit retinal pigment epithelium thinning, Bruch's membrane thickening, lipid accumulation, enhanced C5b‐9 deposition ...
Sergio Recalde +9 more
wiley +1 more source
Food Safety and Health, EarlyView.Industrial manufacturing processes have transformative effects on the components of food matrix. These transformations in food matrix and components have direct implications for quality assurance (QA), food quality (e.g., nutritional profile and texture), and food safety management (e.g., pathogen control and toxin mitigation). ABSTRACT This systematic
Chinaza Godswill Awuchi
wiley +1 more source
Chronic Cold Stress Alters the Skin Mucus Interactome in a Temperate Fish Model
Frontiers in Physiology, 2019 Temperate fish are particularly sensitive to low temperatures, especially in the northern Mediterranean area, where the cold season decreases fish-farm production and affects fish health.
Ignasi Sanahuja +4 more
doaj +1 more source
Acta Ortopédica Brasileira, 2022 Introduction During open surgical dislocated hip reduction, several anatomical structures, such as the round ligament, are approached. However, there is controversy over both the possibility of preserving the ligament and its functional importance ...
ALEXANDRE ZUCCON +5 more
doaj +1 more source
Guaiacol peroxidase zymography for the undergraduate laboratory [PDF]
Biochemistry and Molecular Biology Education, 2014 AbstractThis laboratory exercise presents a novel way to introduce undergraduate students to the specific detection of enzymatic activity by electrophoresis. First, students prepare a crude peroxidase extract and then analyze the homogenate via electrophoresis.
Jeff, Wilkesman +3 more
openaire +2 more sources
Semina: Ciências Agrárias, 2020 Caprine arthritis encephalitis is a lentiviral disease that leads to considerable losses in goat farming. In the acute phase of viral infection, though antiviral antibodies are produced by the host’s immune system, they are not sufficient to be detected ...
Ylana Santos de Galiza +6 more
doaj +1 more source
Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography [PDF]
Journal of the Serbian Chemical Society, 2019 A simple and reliable method for the determination of the concentration and function of alpha-2-macroglobulin (α2M) by zymography was developed. The method is based on the covalent binding of α2M and trypsin followed by non-reducing PAGE and zymography ...
Gligorijević Nikola +4 more
doaj +1 more source