Results 31 to 40 of about 21,615 (216)

Protein engineering of human tissue type plasminogen activator (tPA) using active site of plant-cuscutain enzyme to increase its permanent and activity [PDF]

مجله بیوتکنولوژی کشاورزی, 2017
tPA is a fibrinolitic agent that cause dissolves of blood clots and used in stroke and cardiovascular diseases. Cuscutain is a stable and highly active protease in plants such as dodder, and parasite plants using this enzyme to penetrate into host ...
H seyfi, Kh Piri, M Amini
doaj   +1 more source

Densitometry measurements in zymography and reverse zymography gels.

, 2014
Densitometry measurements in zymography and reverse zymography gels.
Nikolaos P. E. Kadoglou (635739), Nikolaos Kostomitsopoulos (635742), Petros Moustardas (635738), Alkiviadis Kostakis (635744), Alkistis Kapelouzou (635741), Panayotis E. Karayannacos (635743), Christos D. Liapis (635745), Michalis Katsimpoulas (635740)   +7 more
core   +1 more source

Evaluation of matrix metalloproteinase and cysteine cathepsin activity in dentin hybrid layer by gelatin zymography

Indian Journal of Dental Research, 2016
Aim: The aim of this study was to comparatively assess the gelatinolytic activity of matrix metalloproteinases(MMPs) and Cysteine Cathepsins (CCs) in the adhesive interface using etch and rinse adhesive at different time intervals using zymographic ...
Sekar Mahalaxmi, Manavalan Madhana Madhubala, Mahendran Jayaraman, Shanmugasundaram Sathyakumar   +3 more
doaj   +1 more source

Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms’ Lytic Activity Using Mass Spectrometry and Zymography

Toxins, 2017
Background: Medusozoans utilize explosively discharging penetrant nematocysts to inject venom into prey. These venoms are composed of highly complex proteins and peptides with extensive bioactivities, as observed in vitro.
Yang Yue, Huahua Yu, Rongfeng Li, Ronge Xing, Song Liu, Kecheng Li, Xueqin Wang, Xiaolin Chen, Pengcheng Li   +8 more
doaj   +1 more source

Gelatinase activities and TIMP-2 serum level in alcohol cirrhosis and chronic pancreatitis

Current Issues in Pharmacy and Medical Sciences, 2013
There are some divergent data concerning the role of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of MMP (TIMP)-2 in the pathogenesis of alcoholic cirrhosis (AC) and chronic pancreatitis (CP).
Agnieszka Mądro, Joanna Kurzepa, Grażyna Czechowska, Maria Słomka, Krzysztof Celiński, Jacek Kurzepa   +5 more
doaj   +1 more source

In Drosophila Hemolymph, Serine Proteases Are the Major Gelatinases and Caseinases

Insects
After separation on gel zymography, Drosophila melanogaster hemolymph displays gelatinase and caseinase bands of varying sizes, ranging from over 140 to 25 kDa.
Jean-Luc Gatti, Séverine Lemauf, Maya Belghazi, Laury Arthaud, Marylène Poirié   +4 more
doaj   +1 more source

Detection of Functional Matrix Metalloproteinases by Zymography [PDF]

Journal of Visualized Experiments, 2010
Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases. They degrade proteins by cleavage of peptide bonds. More than twenty MMPs have been identified and are separated into six groups based on their structure and substrate specificity (collagenases, gelatinases, membrane type [MT-MMP], stromelysins, matrilysins, and others).
Hu, Xueyou, Beeton, Christine
openaire   +2 more sources

Chemistry Underlying the Behavior of Food and Agricultural Components During Industrial Manufacturing Processes and Its Implications in Quality Assurance, Food Quality, and Food Safety Management: A Systematic Review

Food Safety and Health, EarlyView.
Industrial manufacturing processes have transformative effects on the components of food matrix. These transformations in food matrix and components have direct implications for quality assurance (QA), food quality (e.g., nutritional profile and texture), and food safety management (e.g., pathogen control and toxin mitigation). ABSTRACT This systematic
Chinaza Godswill Awuchi
wiley   +1 more source

Reappraisal of Quantitative Gel Zymography for Matrix Metalloproteinases

, 2014
Background: The determination of matrix metalloproteases (MMPs) is relevant in many pathophysiological conditions, especially if associated with extracellular matrix remodeling; however, the results obtained are closely linked to the method used and are ...
D'Amico A, Del Ry S, Prescimone T, Caselli C, Cabiati M, Giannessi D, Tognotti D   +6 more
core   +1 more source

Environmental Identification of Novel Enzymes for Polyurethane and Polyamide Degradation

Angewandte Chemie, Volume 138, Issue 35, 24 August 2026.
Heteroatom‐containing plastics such as polyurethanes and nylons are produced at scale yet remain largely unrecycled. Biocatalysts offer a promising route to depolymerization under mild conditions. Here, we present a screening platform for the discovery of novel polyurethane‐ and nylon‐degrading enzymes from environmental samples and demonstrate ...
Malthe Kjær Bendtsen, Andreas Møllebjerg, Samuel Peña‐Díaz, Rosie Graham, Nicolai Claus Petersen, Bjørk Nolsøe Isaksen, Mathias Carstensen, Martin B. Johansen, Andreas Sommerfeldt, Allan R. Petersen, Iddi Khamisi Chuma, Cecilie Ryberg, Thomas Rea Wittenborn, Virginia Gichuru, Huabing Wang, Carsten Scavenius, Alexander Sandahl, Daniel E. Otzen   +17 more
wiley   +2 more sources