Results 21 to 30 of about 14,446 (183)
Protein engineering of human tissue type plasminogen activator (tPA) using active site of plant-cuscutain enzyme to increase its permanent and activity [PDF]
مجله بیوتکنولوژی کشاورزی, 2017 tPA is a fibrinolitic agent that cause dissolves of blood clots and used in stroke and cardiovascular diseases. Cuscutain is a stable and highly active protease in plants such as dodder, and parasite plants using this enzyme to penetrate into host ...
H seyfi, Kh Piri, M Amini
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Physiological Difference of Estrus and Pregnant Ovary in Korean Water Deer
Journal of Animal Reproduction and Biotechnology, 2014 To describe the macroscopic anatomy and ovarian-physiological difference of the genital organs of the female Korean water deer, organs from captured animals in a wild area of Korea were dissected.
Sang-Hwan Kim +4 more
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Ciência Rural, 2013 Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted ...
Luciane Maria Laskoski +7 more
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Zymography: developing of the enzyme soil activity visualization method
Бюллетень Почвенного института им. В.В. Докучаева, 2023 The enzymes produced by the soil biota are a key link in the regulation of biochemical processes. The soil enzyme activity can be visualized with zymography, a method based on using fluorescent substrates and obtaining two-dimensional images (zymograms).
M. V. Timofeeva +4 more
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Detection of Functional Matrix Metalloproteinases by Zymography [PDF]
Journal of Visualized Experiments, 2010 Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases. They degrade proteins by cleavage of peptide bonds. More than twenty MMPs have been identified and are separated into six groups based on their structure and substrate specificity (collagenases, gelatinases, membrane type [MT-MMP], stromelysins, matrilysins, and others).
Hu, Xueyou, Beeton, Christine
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Detection of Matrix Metalloproteinases by Zymography [PDF]
, 2017 Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis.
Rajeev B, Tajhya +2 more
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In Drosophila Hemolymph, Serine Proteases Are the Major Gelatinases and Caseinases
InsectsAfter separation on gel zymography, Drosophila melanogaster hemolymph displays gelatinase and caseinase bands of varying sizes, ranging from over 140 to 25 kDa.
Jean-Luc Gatti +4 more
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Zymography for Picogram Detection of Lipase and Esterase Activities
Molecules, 2021 Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity ...
Andre Mong Jie Ng +2 more
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FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
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Animal, 2018 Previously, polyunsaturated fatty acids (PUFA) from linseed oil were effectively protected (>80%) against biohydrogenation through polyphenol-oxidase-mediated protein crosslinking of an emulsion, prepared with polyphenol oxidase (PPO) extract from potato
N. De Neve +5 more
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