Results 21 to 30 of about 14,483 (182)
Protein engineering of human tissue type plasminogen activator (tPA) using active site of plant-cuscutain enzyme to increase its permanent and activity [PDF]
مجله بیوتکنولوژی کشاورزی, 2017 tPA is a fibrinolitic agent that cause dissolves of blood clots and used in stroke and cardiovascular diseases. Cuscutain is a stable and highly active protease in plants such as dodder, and parasite plants using this enzyme to penetrate into host ...
H seyfi, Kh Piri, M Amini
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Zymography: developing of the enzyme soil activity visualization method
Бюллетень Почвенного института им. В.В. Докучаева, 2023 The enzymes produced by the soil biota are a key link in the regulation of biochemical processes. The soil enzyme activity can be visualized with zymography, a method based on using fluorescent substrates and obtaining two-dimensional images (zymograms).
M. V. Timofeeva +4 more
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Indian Journal of Dental Research, 2016 Aim: The aim of this study was to comparatively assess the gelatinolytic activity of matrix metalloproteinases(MMPs) and Cysteine Cathepsins (CCs) in the adhesive interface using etch and rinse adhesive at different time intervals using zymographic ...
Sekar Mahalaxmi +3 more
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Detection of Matrix Metalloproteinases by Zymography [PDF]
, 2017 Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis.
Rajeev B, Tajhya +2 more
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Toxins, 2017 Background: Medusozoans utilize explosively discharging penetrant nematocysts to inject venom into prey. These venoms are composed of highly complex proteins and peptides with extensive bioactivities, as observed in vitro.
Yang Yue +8 more
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Detection of Functional Matrix Metalloproteinases by Zymography [PDF]
Journal of Visualized Experiments, 2010 Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases. They degrade proteins by cleavage of peptide bonds. More than twenty MMPs have been identified and are separated into six groups based on their structure and substrate specificity (collagenases, gelatinases, membrane type [MT-MMP], stromelysins, matrilysins, and others).
Hu, Xueyou, Beeton, Christine
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Gelatinase activities and TIMP-2 serum level in alcohol cirrhosis and chronic pancreatitis
Current Issues in Pharmacy and Medical Sciences, 2013 There are some divergent data concerning the role of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of MMP (TIMP)-2 in the pathogenesis of alcoholic cirrhosis (AC) and chronic pancreatitis (CP).
Agnieszka Mądro +5 more
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In Drosophila Hemolymph, Serine Proteases Are the Major Gelatinases and Caseinases
InsectsAfter separation on gel zymography, Drosophila melanogaster hemolymph displays gelatinase and caseinase bands of varying sizes, ranging from over 140 to 25 kDa.
Jean-Luc Gatti +4 more
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Zymography for Picogram Detection of Lipase and Esterase Activities
Molecules, 2021 Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity ...
Andre Mong Jie Ng +2 more
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Animal, 2018 Previously, polyunsaturated fatty acids (PUFA) from linseed oil were effectively protected (>80%) against biohydrogenation through polyphenol-oxidase-mediated protein crosslinking of an emulsion, prepared with polyphenol oxidase (PPO) extract from potato
N. De Neve +5 more
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