An Inexpensive Staining Alternative for Gelatin Zymography Gels [PDF]
Methods and Protocols, 2019 Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel.
Christian Wechselberger +2 more
doaj +3 more sources
Comparison of Single-Step Methods to Enrich Lipase Concentrations in Bacterial Cell Lysates
Brazilian Archives of Biology and Technology, 2021 Lipases are currently used in food technology for the modification of fats and oils. The thermal stability of lipase is an essential characteristic for this application.
Marie Kathleen Walsh +1 more
doaj +1 more source
Identification of a novel 82 kDa proMMP-9 species associated with the surface of leukaemic cells [PDF]
, 2007 MMP-9 (matrix metalloproteinase 9) plays a critical role in tumour progression. Although the biochemical properties of the secreted form of proMMP-9 are well characterized, little is known about the function and activity of cell surface-associated proMMP-
Egea, Virginia +6 more
core +1 more source
Removal of luminal content protects the small intestine during hemorrhagic shock but is not sufficient to prevent lung injury. [PDF]
, 2013 The small intestine plays a key role in the pathogenesis of multiple organ failure following circulatory shock. Current results show that reduced perfusion of the small intestine compromises the mucosal epithelial barrier, and the intestinal contents ...
Altshuler, Angelina E +5 more
core +2 more sources
Memorias do Instituto Oswaldo Cruz, 2012 Angiostrongylus costaricensis is a nematode that causes abdominal angiostrongyliasis, a widespread human parasitism in Latin America. This study aimed to characterize the protease profiles of different developmental stages of this helminth.
Karina Mastropasqua Rebello +7 more
doaj +1 more source
Modulation of autocrine TNF-α-stimulated matrix metalloproteinase 9 (MMP-9) expression by mitogenactivated protein kinases in THP-1 monocytic cells [PDF]
, 2006 Matrix metalloproteinase 9 (MMP-9) is implicated in various physiological processes by its ability to degrade the extracellular matrix (ECM) and process multiple regulatory proteins. Normally, MMP-9 expression is tightly controlled in cells. Sustained or
Bogoyevitch +13 more
core +1 more source
Acute- and late-phase matrix metalloproteinase (MMP)-9 activity is comparable in female and male rats after peripheral nerve injury. [PDF]
, 2018 BACKGROUND:In the peripheral nerve, pro-inflammatory matrix metalloproteinase (MMP)-9 performs essential functions in the acute response to injury. Whether MMP-9 activity contributes to late-phase injury or whether MMP-9 expression or activity after ...
Angert, Mila +6 more
core +2 more sources
Toxins, 2022 Viperidae snakes are the most important agents of snakebites in Brazil. The protein composition of snake venoms has been frequently analyzed by means of electrophoretic techniques, but the interaction of proteins in venoms has barely been addressed.
Natacha Ferreira de Oliveira +2 more
doaj +1 more source
Matrix metalloproteinases-2,-3,-7,-9 and-10, but not MMP-11, are differentially expressed in normal, benign tumorigenic and malignant human keratinocyte cell lines [PDF]
, 2000 In order to investigate the correlations between constitutive proteinase expression and the degree of tumorigenicity of cancer cells we have studied a model system of three keratinocyte cell lines.
Bachmeier, B. E. +4 more
core +1 more source
Detection of proteolytic activity by covalent tethering of fluorogenic substrates in zymogram gels
BioTechniques, 2018 Current zymographic techniques detect only a subset of known proteases due to the limited number of native proteins that have been optimized for incorporation into polyacrylamide gels.
Ameya A Deshmukh +2 more
doaj +1 more source