Results 11 to 20 of about 21,615 (216)

Zymography of Metalloproteinases

Current Protocols in Protein Science, 2003
AbstractZymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly ...
Troeberg, L, Nagase, H
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In situ zymography: topographical considerations

Journal of Biochemical and Biophysical Methods, 2001
In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard,
Mungall, BA, Pollitt, CC
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In Situ Zymography

, 2003
In situ zymography is a unique laboratory technique that enables the localisation of matrix-degrading metalloproteinase (MMP) activity in histological sections. Frozen sections are placed on glass slides coated with fluorescently labelled matrix proteins.
George, SJ, Johnson, JL
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Zymography: developing of the enzyme soil activity visualization method [PDF]

Бюллетень Почвенного института им. В.В. Докучаева, 2023
The enzymes produced by the soil biota are a key link in the regulation of biochemical processes. The soil enzyme activity can be visualized with zymography, a method based on using fluorescent substrates and obtaining two-dimensional images (zymograms).
M. V. Timofeeva, K. N. Abrosimov, A. V. Yudina, D. S. Fomin, V. V. Klyueva   +4 more
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Zymography methods for visualizing hydrolytic enzymes

Nature Methods, 2013
Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful, but often misinterpreted, tool yielding information on potential hydrolytic activities, enzyme forms and the locations of active enzymes.
Jennifer, Vandooren, Nathalie, Geurts, Erik, Martens, Philippe E, Van den Steen, Ghislain, Opdenakker   +4 more
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Incorporation of β-actin loading control into zymography

Journal of Biological Methods, 2016
Gelatin zymography and immunoblot are widely used gel electrophoresis techniques to study matrix metalloproteinases-2 and -9. Each method has its advantages and disadvantages. Zymography is exquisitely sensitive but offers no loading control to ensure equal sample loading.
Govindasamy, Natasha, Yan, MengJie, Jurasz, Paul   +2 more
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An improved collagen zymography approach for evaluating the collagenases MMP-1, MMP-8, and MMP-13

BioTechniques, 2017
Collagen zymography is an SDS-PAGE–based method for detecting both the proenzyme and active forms of collagenases. Although collagen zymography is used for assessment of the matrix metalloproteinases MMP-1 and MMP-13, it can be difficult to detect these ...
Seniz Inanc, Didem Keles, Gulgun Oktay
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Electrophoretic Zymography of Protease Using Azocasein

Zymography is an electrophoretic technique used to demonstrate enzyme activity during separation in polyacrylamide gels under nonreducing conditions. This kind of analysis can be used in association with electrophoresis (SDS-PAGE) to show which protein is correlated with a given activity. Proteases are the most used enzymes for this technique since the
Carvalho-Silva, Jônatas, Fernandes, Lígia M. G., Converti, Attilio, Porto, Tatiana S.   +3 more
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Comparison of Single-Step Methods to Enrich Lipase Concentrations in Bacterial Cell Lysates

Brazilian Archives of Biology and Technology, 2021
Lipases are currently used in food technology for the modification of fats and oils. The thermal stability of lipase is an essential characteristic for this application.
Marie Kathleen Walsh, Teif AbdulQani Najm   +1 more
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Proteolytic activity in the adult and larval stages of the human roundworm parasite Angiostrongylus costaricensis

Memorias do Instituto Oswaldo Cruz, 2012
Angiostrongylus costaricensis is a nematode that causes abdominal angiostrongyliasis, a widespread human parasitism in Latin America. This study aimed to characterize the protease profiles of different developmental stages of this helminth.
Karina Mastropasqua Rebello, Caroline Reis de Siqueira, Erika Louise Ribeiro, Richard Hemmi Valente, Ester Maria Mota, Jonas Perales, Ana Gisele da Costa Neves-Ferreira, Henrique Leonel Lenzi   +7 more
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