Results 11 to 20 of about 14,483 (182)

Lipocalin‐2 activates hepatic stellate cells and promotes nonalcoholic steatohepatitis in high‐fat diet–fed Ob/Ob mice

open access: yesHepatology, EarlyView., 2022
Graphical summary of obesity‐induced NASH progression by LCN2 targeted to HSC activation. Abstract Background and Aims In obesity and type 2 diabetes mellitus, leptin promotes insulin resistance and contributes to the progression of NASH via activation of hepatic stellate cells (HSCs).
Kyung Eun Kim   +12 more
wiley   +1 more source

Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

open access: yesMarine Drugs, 2020
The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive ...
Eugenia Baena   +9 more
doaj   +1 more source

Zymography of Metalloproteinases

open access: yesCurrent Protocols in Protein Science, 2003
AbstractZymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly ...
Troeberg, L, Nagase, H
openaire   +2 more sources

Two-Dimensional Blue Native/SDS Polyacrylamide Gel Electrophoresis for Analysis of Brazilian Bothrops Snake Venoms

open access: yesToxins, 2022
Viperidae snakes are the most important agents of snakebites in Brazil. The protein composition of snake venoms has been frequently analyzed by means of electrophoretic techniques, but the interaction of proteins in venoms has barely been addressed.
Natacha Ferreira de Oliveira   +2 more
doaj   +1 more source

Tissue Inhibitor of Matrix Metalloproteinases-1 (TIMP-1) and Pulmonary Involvement in COVID-19 Pneumonia

open access: yesBiomolecules, 2023
Background: The aim of the study was to longitudinally evaluate the association between MMP-2, MMP-9, TIMP-1 and chest radiological findings in COVID-19 patients.
Maria Antonella Zingaropoli   +17 more
doaj   +1 more source

Physiological Difference of Estrus and Pregnant Ovary in Korean Water Deer

open access: yesJournal of Animal Reproduction and Biotechnology, 2014
To describe the macroscopic anatomy and ovarian-physiological difference of the genital organs of the female Korean water deer, organs from captured animals in a wild area of Korea were dissected.
Sang-Hwan Kim   +4 more
doaj   +1 more source

Environmental Identification of Novel Enzymes for Polyurethane and Polyamide Degradation

open access: yesAngewandte Chemie, EarlyView.
Heteroatom‐containing plastics such as polyurethanes and nylons are produced at scale yet remain largely unrecycled. Biocatalysts offer a promising route to depolymerization under mild conditions. Here, we present a screening platform for the discovery of novel polyurethane‐ and nylon‐degrading enzymes from environmental samples and demonstrate ...
Malthe Kjær Bendtsen   +17 more
wiley   +2 more sources

Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment Expressão de metaloproteinases 2 e 9 no tecido laminar do casco de equinos após obstrução intestinal e tratamento com hidrocortisona

open access: yesCiência Rural, 2013
Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted ...
Luciane Maria Laskoski   +7 more
doaj   +1 more source

Contrasting distribution of enzyme activities in the rhizosphere of European beech and Norway spruce

open access: yesFrontiers in Plant Science, 2022
Recent policies and silvicultural management call for forest regeneration that involve the selection of tree species able to cope with low soil nutrient availability in forest ecosystems.
Bin Song   +5 more
doaj   +1 more source

Detection of proteolytic activity by covalent tethering of fluorogenic substrates in zymogram gels

open access: yesBioTechniques, 2018
Current zymographic techniques detect only a subset of known proteases due to the limited number of native proteins that have been optimized for incorporation into polyacrylamide gels.
Ameya A Deshmukh   +2 more
doaj   +1 more source

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