Zymography - Open Access .click

Methods in Molecular Biology, 2022
Zymography is a powerful technique for the assay of different hydrolases that act upon any biological macromolecule. In particular, zymography is used to assay the activities of serine proteases, e.g., matrix metalloproteinases (MMPs), and reverse zymography is used for tissue inhibitors of metalloproteinases (TIMPs) in multifarious experimental ...
Snehasikta Swarnakar
exaly +3 more sources

Loading-Controlled Zymography

Methods in Molecular Biology
Gelatin zymography is a widely used technique to study matrix metalloproteinases (MMP)-2 and -9, characterized by its simplicity, low cost, and high sensitivity. Zymography can detect picogram quantities of MMPs, which is 100-1000-fold more sensitive than immunoblot.
Jurasz Paul
exaly +3 more sources

RAMA casein zymography: Time‐saving and highly sensitive casein zymography for MMP7 and trypsin

Electrophoresis, 2016
To detect metalloproteinase‐7 (MMP7), zymography is conducted using a casein substrate and conventional CBB stain. It has disadvantages because it is time consuming and has low sensitivity. Previously, a sensitive method to detect MMP7 up to 30 pg was reported, however it required special substrates and complicated handlings.
Yasuhiro Ozeki
exaly +3 more sources

Zymography methods for visualizing hydrolytic enzymes

Nature Methods, 2013
Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful, but often misinterpreted, tool yielding information on potential hydrolytic activities, enzyme forms and the locations of active enzymes.
Jennifer Vandooren +2 more
exaly +3 more sources

electrophoresis, polyacrylamide gel
humans
animals

enzyme assays
3. good health
gelatin

matrix metalloproteinases
peptide hydrolases
substrate specificity