Significance of molecular and zymography profiling for accurate detection of infected sheep with Anaplasma marginale in South Sinai, Egypt. [PDF]
Sayed MA +5 more
europepmc +1 more source
Erratum to "Clinic-ready inhibitor of MMP-9/-12 restores sensory and functional decline in rodent models of spinal cord injury". [PDF]
europepmc +1 more source
Correction: Endogenous SLPI contributes to the regulation of inflammatory responses in peritoneal macrophages by modulating MMP-9 production. [PDF]
Tyshchenko M +5 more
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The Coordinated Interplay Between MMP13 and Pro-Migratory MMPs in Collective Cell Migration of Zebrafish Keratocytes. [PDF]
Uppalapati CK +4 more
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<i>Ferula ammoniacum</i> gum aqueous extract exerts anti-inflammatory and antioxidant mechanisms to combat aluminum chloride-induced Alzheimer's disease in rats: possible involvement of opioid pathways. [PDF]
Fakhri S +6 more
europepmc +1 more source
Inhibition of Metalloproteinases-2, -9, and -14 Suppresses Papillary Thyroid Carcinoma Cell Migration and Invasion. [PDF]
Rocco D, Marotta V, Palumbo D, Vitale M.
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Zymography and Reverse Zymography for Testing Proteases and Their Inhibitors
Methods in Molecular Biology, 2022Zymography is a powerful technique for the assay of different hydrolases that act upon any biological macromolecule. In particular, zymography is used to assay the activities of serine proteases, e.g., matrix metalloproteinases (MMPs), and reverse zymography is used for tissue inhibitors of metalloproteinases (TIMPs) in multifarious experimental ...
Snehasikta Swarnakar
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Amylase zymography was carried out for the detection of amylases produced by a Geobacillus stearothermophilus strain isolated from the Thermal Center "Las Trincheras" in Venezuela. Zymography is an electrophoretic technique used to study hydrolases by means of thin gels containing copolymerized-specific substrates, under nonreducing conditions. In this
Adarelys, Andrades, Lellys M, Contreras
openaire +2 more sources
In situ zymography is a unique laboratory technique that enables the localisation of matrix-degrading metalloproteinase (MMP) activity in histological sections. Frozen sections are placed on glass slides coated with fluorescently labelled matrix proteins.
George, SJ, Johnson, JL
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Zymography and Reverse Zymography for Detecting MMPs, and TIMPs
2003Zymography is the electrophoretic separation of proteins through a polyacrylamide gel containing a proteolytic substrate. After denaturing (but nonreducing) electrophoresis, proteins are renatured and incubated in an appropriate buffer for proteolytic activity. Clear zones of lysis in the stained gel indicated active proteinases.
S P, Hawkes, H, Li, G T, Taniguchi
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