Results 141 to 150 of about 14,446 (183)

Detection and Quantitation of Matrix Metalloproteases by Zymography

2003
Matrix metalloproteases (MMPs) are a family of structurally and functionally related endopeptidases. They have in common a zinc ion at the active site and are released as an inactive proform (zymogen). Proteolytic activation enables MMPs to degrade components of the extracellular matrix such as collagens, fibronectin, and laminin [for review see (1-4)].
T M, Leber, R P, Negus
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Loading-Controlled Zymography

Gelatin zymography is a widely used technique to study matrix metalloproteinases (MMP)-2 and -9, characterized by its simplicity, low cost, and high sensitivity. Zymography can detect picogram quantities of MMPs, which is 100-1000-fold more sensitive than immunoblot.
Aleksandra, Franczak, Paul, Jurasz
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Zymography with Caseogram Prints: Quantification of Pepsinogen

Analytical Biochemistry, 2001
In caseogram prints, a type of zymogram which is designed for the detection of acid proteases, enzyme activity is detected in an overlay gel of agarose containing skim milk. The use of this technique for protease quantification was investigated in this study using pepsinogen as an example protease.
O, Till, E, Baumann, W, Linss
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Zymography in Multiwells for Quality Assessment of Proteinases

2017
Zymography is a well-standardized protocol for the qualitative assessment and analysis of proteinases under specified conditions. However, analysis of a large number of samples simultaneously becomes a challenge when the zymography is carried out by the usual protocol of electrophoresis. This can be overcome by assaying the matrix-degrading proteinases
Ambili, Mechoor, Madathiparambil G, Madanan   +1 more
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Detection of Protease Activity by Fluorescent Peptide Zymography

Journal of Visualized Experiments, 2019
The purpose of this method is to measure the proteolytic activity of complex biological samples. The samples are separated by molecular weight using electrophoresis through a resolving gel embedded with a degradable substrate. This method differs from traditional gel zymography in that a quenched fluorogenic peptide is covalently incorporated into the ...
Ameya A, Deshmukh, Jessica L, Weist, Jennifer L, Leight   +2 more
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Application of Gelatin Zymography in Nanotoxicity Research

2018
Gelatin zymography is a relatively simple, inexpensive, and powerful technique to detect proteolytic enzymes capable of degrading gelatin from various biological sources. It has been used particularly to detect the two members of the matrix metalloproteinase family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading ...
Yue, Zhang, Rong, Wan, Qunwei, Zhang, Yiqun, Mo   +3 more
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Antibody zymography: a novel adaptation of zymography to determine the protease-neutralising potential of specific antibodies and snake antivenoms

Journal of Immunological Methods, 2004
A common problem in the development of antibody-based therapeutics is the selection, usually from a large population, of specific antibodies with the desired function. One of our research objectives is to identify antibodies capable of neutralising the most important haemorrhagic and haemostasis-disruptive proteases from viper venom.
S S, Hasson, R D G, Theakston, R A, Harrison   +2 more
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Recent Updates on Protease Zymography

Proteases, also known as peptidases or proteinases, are enzymes that break down peptide bonds of proteins. It is essential for number of physiological processes like digestion, immune response, blood clotting, and cell regulation. Different versions of the same protease enzyme, or isoforms, result from post-translational modifications, alternative ...
Abinaya, Kanagaraja, Raman, Pachaiappan
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Detection of Gelatinases by Substrate Zymography

Zymography is a method of electrophoretic separation of matrix metalloproteinases (MMPs) in a polyacrylamide gel-containing substrate (called zymogram gel) for the assay of various MMPs in different biological samples. In particular, gelatin-zymography allows to determine simultaneously both active and latent forms of MMP-2 (gelatinase A) and MMP-9 ...
Emanuela, Falcinelli, Paolo, Gresele
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Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

2017
To detect serine protease activity by zymography, casein and CBB stain have been used as a substrate and a detection procedure, respectively. Casein zymography has been using substrate concentration at 1 mg/mL and employing conventional CBB stain. Although ordinary casein zymography provides reproducible results, it has several disadvantages including ...
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