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Cysteine Protease Zymography: Brief Review
2017Cysteine proteases play multiple roles in basically all aspects of physiology and development. In plants, they are involved in growth and development and in accumulation and mobilization of storage proteins. Furthermore, they are engaged in signalling pathways and in the response to biotic and abiotic stresses.
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Zymography: Unveiling Matrix Metalloproteinase Dynamics
Zymography is a laboratory technique used to study the activity of enzymes, particularly proteases and glycosidases, in biological samples. It involves the separation of enzymatic proteins by gel electrophoresis under non-denaturing conditions, followed by their renaturation and incubation with specific substrates that mimic natural substrates.Qinghe, Meng, Adam, Novak, Erin, Rayhill
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Zymography for the Detection of Bacterial Proteases
Proteases serve important functions for eukaryotes and bacteria, such as processing other proteins to activate them or degrading other proteins to create smaller peptides or eliminate malformed proteins. Proteases in pathogenic bacteria can also act as virulence factors by virtue of degradation of host proteins, activating other proteins that then ...openaire +2 more sources
Calpain Zymography: General Methodology and Protocol
2017Casein zymography has become one of the gold standard assays for monitoring mammalian calcium-activated proteases (calpains) in purified enzyme, cell, or tissue samples. This calpain zymography method takes advantages of (1) casein is an excellent substrate for major isoforms of calpains (Calpain-1, 2 and 3), (2) the embedded casein is digested into ...
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Assessment of Matrix Metalloproteinases by Gelatin Zymography
2016Matrix metalloproteinases are endopeptidases responsible for remodeling of the extracellular matrix and have been identified as critical contributors to breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression.
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Electrophoretic Zymography of Protease Using Azocasein
Zymography is an electrophoretic technique used to demonstrate enzyme activity during separation in polyacrylamide gels under nonreducing conditions. This kind of analysis can be used in association with electrophoresis (SDS-PAGE) to show which protein is correlated with a given activity. Proteases are the most used enzymes for this technique since theCarvalho-Silva, Jônatas +3 more
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Enhanced Zymography of Proteases
Analytical Biochemistry, 1993C, Paech, T, Christianson, S, Bläsig
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Fundamentals of Zymography: Applications to the Study of Biological Samples
Zymography is a very useful technique to determine the gelatinolytic activity of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9). This work describes the preparation and use of different biological samples (culture media, cell lysates, tissue extracts, and extracellular vesicles), as well as the determination of gelatinolytic activity in these ...Jorge, Recio-Aldavero +3 more
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Leveraging Zymography to Discover and Investigate Nuclear Proteases
Although many reports over the past few decades have documented protease activity within the nucleus of various eukaryotic cells and tissues, the identity and biological significance of these nuclear proteases remain largely unknown. In-gel zymography is an elegant, sensitive, and inexpensive technique to identify and investigate proteases within ...Benjamin H, Weekley +2 more
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