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Matrix metalloproteinase activity assays: Importance of zymography
Journal of Pharmacological and Toxicological Methods, 2010Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases capable of degrading extracellular matrix, including the basement membrane. MMPs are associated with various physiological processes such as morphogenesis, angiogenesis, and tissue repair.
Peter Ferdinandy
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Zymography and Reverse Zymography for Testing Proteases and Their Inhibitors
2022Zymography is a powerful technique for the assay of different hydrolases that act upon any biological macromolecule. In particular, zymography is used to assay the activities of serine proteases, e.g., matrix metalloproteinases (MMPs), and reverse zymography is used for tissue inhibitors of metalloproteinases (TIMPs) in multifarious experimental ...
Preety, Choudhary +2 more
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2017
The technique described here, transfer zymography, was developed to overcome two limitations of conventional zymography. When proteolytic enzymes are resolved by nonreducing SDS-PAGE into a polyacrylamide gel with copolymerized protein substrate, the presence of the protein substrate can result in anomalous, often slower, migration of the protease and ...
Daniel, Pan +2 more
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The technique described here, transfer zymography, was developed to overcome two limitations of conventional zymography. When proteolytic enzymes are resolved by nonreducing SDS-PAGE into a polyacrylamide gel with copolymerized protein substrate, the presence of the protein substrate can result in anomalous, often slower, migration of the protease and ...
Daniel, Pan +2 more
openaire +2 more sources
2017
Amylase zymography was carried out for the detection of amylases produced by a Geobacillus stearothermophilus strain isolated from the Thermal Center "Las Trincheras" in Venezuela. Zymography is an electrophoretic technique used to study hydrolases by means of thin gels containing copolymerized-specific substrates, under nonreducing conditions. In this
Adarelys, Andrades, Lellys M, Contreras
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Amylase zymography was carried out for the detection of amylases produced by a Geobacillus stearothermophilus strain isolated from the Thermal Center "Las Trincheras" in Venezuela. Zymography is an electrophoretic technique used to study hydrolases by means of thin gels containing copolymerized-specific substrates, under nonreducing conditions. In this
Adarelys, Andrades, Lellys M, Contreras
openaire +2 more sources
2017
Zymography, the detection, identification, and even quantification of enzyme activity fractionated by gel electrophoresis, has received increasing attention in the last years, as revealed by the number of articles published. A number of enzymes are routinely detected by zymography, especially with clinical interest.
Jeff, Wilkesman, Liliana, Kurz
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Zymography, the detection, identification, and even quantification of enzyme activity fractionated by gel electrophoresis, has received increasing attention in the last years, as revealed by the number of articles published. A number of enzymes are routinely detected by zymography, especially with clinical interest.
Jeff, Wilkesman, Liliana, Kurz
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Electrophoretic transfer protein zymography
Analytical Biochemistry, 2011Zymography detects and characterizes proteolytic enzymes by electrophoresis of protease-containing samples into a nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel containing a copolymerized protein substrate. The usefulness of zymography for molecular weight determination and proteomic analysis is hampered by the ...
Daniel, Pan +4 more
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Zymography and Reverse Zymography for Detecting MMPs, and TIMPs
2003Zymography is the electrophoretic separation of proteins through a polyacrylamide gel containing a proteolytic substrate. After denaturing (but nonreducing) electrophoresis, proteins are renatured and incubated in an appropriate buffer for proteolytic activity. Clear zones of lysis in the stained gel indicated active proteinases.
S P, Hawkes, H, Li, G T, Taniguchi
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2003
In situ zymography is a unique laboratory technique that enables the localisation of matrix-degrading metalloproteinase (MMP) activity in histological sections. Frozen sections are placed on glass slides coated with fluorescently labelled matrix proteins.
George, SJ, Johnson, JL
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In situ zymography is a unique laboratory technique that enables the localisation of matrix-degrading metalloproteinase (MMP) activity in histological sections. Frozen sections are placed on glass slides coated with fluorescently labelled matrix proteins.
George, SJ, Johnson, JL
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In situ zymography: topographical considerations
Journal of Biochemical and Biophysical Methods, 2001In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard,
Mungall, BA, Pollitt, CC
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1999
Proteases and their inhibitors play an important role in the extracellular matrix (ECM) remodeling that occurs in a number of physiological and pathological processes. These include such diverse processes as trophoblast implantation, mammary gland involution, rheumatoid arthritis and tumor cell invasion (Liotta et al. 1991a; Liotta et al.
Gary W. Oliver +2 more
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Proteases and their inhibitors play an important role in the extracellular matrix (ECM) remodeling that occurs in a number of physiological and pathological processes. These include such diverse processes as trophoblast implantation, mammary gland involution, rheumatoid arthritis and tumor cell invasion (Liotta et al. 1991a; Liotta et al.
Gary W. Oliver +2 more
openaire +1 more source