Results 191 to 200 of about 27,806 (225)

Calpain Zymography: General Methodology and Protocol

2017
Casein zymography has become one of the gold standard assays for monitoring mammalian calcium-activated proteases (calpains) in purified enzyme, cell, or tissue samples. This calpain zymography method takes advantages of (1) casein is an excellent substrate for major isoforms of calpains (Calpain-1, 2 and 3), (2) the embedded casein is digested into ...
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Recent Updates on Protease Zymography

Proteases, also known as peptidases or proteinases, are enzymes that break down peptide bonds of proteins. It is essential for number of physiological processes like digestion, immune response, blood clotting, and cell regulation. Different versions of the same protease enzyme, or isoforms, result from post-translational modifications, alternative ...
Abinaya, Kanagaraja, Raman, Pachaiappan
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Application of Gelatin Zymography in Nanotoxicity Research

2018
Gelatin zymography is a relatively simple, inexpensive, and powerful technique to detect proteolytic enzymes capable of degrading gelatin from various biological sources. It has been used particularly to detect the two members of the matrix metalloproteinase family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading ...
Yue, Zhang, Rong, Wan, Qunwei, Zhang, Yiqun, Mo   +3 more
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Detection and Quantitation of Matrix Metalloproteases by Zymography

2003
Matrix metalloproteases (MMPs) are a family of structurally and functionally related endopeptidases. They have in common a zinc ion at the active site and are released as an inactive proform (zymogen). Proteolytic activation enables MMPs to degrade components of the extracellular matrix such as collagens, fibronectin, and laminin [for review see (1-4)].
T M, Leber, R P, Negus
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Cell In Situ Zymography: Imaging Enzyme–Substrate Interactions

2017
Zymography has long been used for the detection of substrate-specific enzyme activity. In situ zymography (ISZ), an adaptation from the conventional substrate zymography, is a widely employed technique useful for the detection, localization, and estimation of enzyme-substrate interactions in tissues. Here, we describe a protocol to detect 'in position'
Aastha, Chhabra, Vibha, Rani
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Electrophoretic Zymography of Protease Using Azocasein

Zymography is an electrophoretic technique used to demonstrate enzyme activity during separation in polyacrylamide gels under nonreducing conditions. This kind of analysis can be used in association with electrophoresis (SDS-PAGE) to show which protein is correlated with a given activity. Proteases are the most used enzymes for this technique since the
Carvalho-Silva, Jônatas, Fernandes, Lígia M. G., Converti, Attilio, Porto, Tatiana S.   +3 more
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Detection of Gelatinases by Substrate Zymography

Zymography is a method of electrophoretic separation of matrix metalloproteinases (MMPs) in a polyacrylamide gel-containing substrate (called zymogram gel) for the assay of various MMPs in different biological samples. In particular, gelatin-zymography allows to determine simultaneously both active and latent forms of MMP-2 (gelatinase A) and MMP-9 ...
Emanuela, Falcinelli, Paolo, Gresele
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Measuring Matrix Metalloproteinases Activity by Gelatin Zymography

Gelatin zymography is a widely popular method due to its simplicity, low cost, and quick results, for studying gelatinases in various biological systems. Zymography can detect both the pro and active forms of matrix metalloproteinases MMP-2 (gelatinase A) and MMP-9 (gelatinase B).
Kelli C, Micocci, Uliana S, Stotzer, Milene N O, Moritz   +2 more
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Assessment of Matrix Metalloproteinases by Gelatin Zymography

2016
Matrix metalloproteinases are endopeptidases responsible for remodeling of the extracellular matrix and have been identified as critical contributors to breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression.
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Gelatin Zymography of Proteases in Chronic Wound Samples

Cellular processes such as tissue regeneration, inflammation, and migration require the proteolysis of the extracellular matrix and the proteolytic activation of signaling molecules. A widely used and accessible technique for studying these processes is gelatin zymography, particularly for investigating matrix metalloproteinases (MMPs), though it is ...
Vojtěch, Pavlík, Aneta, Horčičková
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