Purification and characterization of α-galactosidase from Aspergillus niger v. Tiegh RM48
浙江大学学报. 农业与生命科学版, 2009 An extracellular α-galactosidase from Aspergillus niger v. Tiegh RM48 was purified to apparent homogeneity by ammonium sulfate (80% saturation) precipitation, Phenyl Sepharose CL-4B hydrophobic column chromatography and Sephadex G-100 filtration ...
XU Yao-xing +4 more
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Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]
FEMS Microbiology Letters, 2008 A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao +8 more
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Production of a Highly Protease-Resistant Fungal α-Galactosidase in Transgenic Maize Seeds for Simplified Feed Processing. [PDF]
PLoS ONE, 2015 Raffinose-family oligosaccharide (RFO) in soybeans is one of the major anti-nutritional factors for poultry and livestocks. α-Galactosidase is commonly supplemented into the animal feed to hydrolyze α-1,6-galactosidic bonds on the RFOs.
Wenxia Yang +9 more
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High gene expression inEscherichia coliof recombinant alginate lyase as a fused protein with β-galactosidase α-peptide [PDF]
FEMS Microbiology Letters, 1995 Escherichia coli LE392 (pAL28) was previously isolated as a positive clone harboring the alginate lyase gene (aly) from an alginate-degrading strain, Pseudomonas sp. OS-ALG-9. The plasmid pAL205, one of the constructs obtained after successive subcloning of pAL28, gave the highest expression of aly in E. coli cells.
K, Fujiyama +3 more
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Antigenicity of a viral peptide displayed on β-galactosidase fusion proteins is influenced by the presence of the homologous partner protein [PDF]
FEMS Microbiology Letters, 1996 Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competitive ELISA using a panel of seven ...
J L, Corchero, A, Villaverde
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Improving the Secretory Expression of an -Galactosidase from Aspergillus niger in Pichia pastoris. [PDF]
PLoS ONE, 2016 α-Galactosidases are broadly used in feed, food, chemical, pulp, and pharmaceutical industries. However, there lacks a satisfactory microbial cell factory that is able to produce α-galactosidases efficiently and cost-effectively to date, which prevents ...
Xianliang Zheng +6 more
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Genomic Features of a Food-Derived Pseudomonas aeruginosa Strain PAEM and Biofilm-Associated Gene Expression under a Marine Bacterial α-Galactosidase. [PDF]
Int J Mol Sci, 2020 Balabanova L +8 more
europepmc +3 more sources
Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects [PDF]
FEMS Microbiology Ecology, 2005 Previous studies suggest that, besides the maldigestion of lactose in the small intestine, the colonic processing of lactose might play a role in lactose intolerance. beta-Galactosidase is the bacterial enzyme which catalyzes the first step of lactose fermentation in the colon.
Tao, H +3 more
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Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]
Arthropods, 2020 The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei +2 more
doaj
Future Foods, 2021 Intake of Fermentable Oligo,- Di,- Monosaccharides, And Polyols, abbreviated as FODMAP, is known to worsen gastrointestinal symptoms in people with functional bowel disorders.
Antti Nyyssölä +7 more
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