Results 61 to 70 of about 937,878 (172)

Hierarchical Nanoplatform Integrating Oxidative–Acidic Microenvironment Remodeling and Bone Homeostasis Modulation for Diabetic Bone Defect Therapy

Advanced Science, EarlyView.
A hierarchical CM/LDH nanoplatform integrates ROS‐scavenging Ce‐MOF nanozymes with acid‐neutralizing CaAl‐LDH and is delivered within GelMA hydrogel to diabetic bone defects. By remodeling the oxidative‐acidic microenvironment, preserving mitochondrial homeostasis, and suppressing mtDNA–cGAS–STING inflammatory signaling, CM/LDH reduces proinflammatory ...
Xu Chen, Xingyu Zhu, Zhixin Qiu, Yijia Zhu, Xinhui Zheng, Dongqi Fan, Jinglei Zhan, Congxiu Mao, Ping Ji, Tao Chen, Qingqing He   +10 more
wiley   +1 more source

Broad‐Spectrum, Cell Envelope‐Active Marinocyclin Antibiotics From a Coral‐Derived Bacterium Are Effective Against Colistin‐Resistant Bacteria

Angewandte Chemie, EarlyView.
Marinocyclins, polycationic macrocyclic depsipeptides, were discovered by genome mining from the marine coral‐derived bacterium Aquimarina megaterium. The compounds have broad‐spectrum activity against gram‐positive and gram‐negative ESKAPE bacteria.
Clara Chepkirui, Sandra Silva, Jan Straetener, Chandrashekhar Padhi, Tina Keller‐Costa, Tess Lamer, Laura Reinke, Heike Brötz‐Oesterhelt, Rodrigo Costa, Jörn Piel   +9 more
wiley   +2 more sources

Purification and characterization of α-D-galactosidase from lactobacillus sporogenes

, 2010
碩士本實驗以基因工程的方法，將稻米α-galactosidase基因接到質 體上(pLDHEB)，並轉殖入乳酸菌(Lactobacillus sporogenes)表現出較多的α-半乳糖水解酵素。經由大量製備純化後予以定性和定量分析，並測試B型紅血球轉變為O型紅血球之情形。 在最佳條件下培養乳酸菌，並利用超音波細胞破碎後，1升的菌液可以得到1.2 g、40單位的粗製蛋白質。再由液相層析方法使用分子篩管柱層析(Sephadex G-150、Sephacryl S-100)、陰離子交換樹脂(DEAE ...
陳奕任; Chen, Yi-jen
core   +1 more source

Purification and some properties of phospho-β-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201 [PDF]

FEMS Microbiology Letters, 2002
Phospho-beta-galactosidase (P-beta-gal; EC 3.2.1.85) is induced during growth of Leptotrichia buccalis ATCC 14201 on lactose and lactulose. The enzyme has been purified to electrophoretic homogeneity (M(r) approximately 53 kDa, pI approximately 4.8), and kinetic parameters have been determined using the chromogenic analog o-nitrophenyl-beta-D ...
openaire   +2 more sources

Persistent Zn2+ Influx‐Mediated Deacetylation of ANXA2 Regulates the “Zn2+‐Autophagic Flux” and Alleviates Intervertebral Disc Degeneration

Advanced Science, EarlyView.
Zinc transporter SLC39A14 sustains intracellular Zn2+ levels to activate SIRT3‑dependent ANXA2 deacetylation at K302. This modification weakens ANXA2‑mTOR binding and releases autophagic‑flux suppression in nucleus pulposus cells. Dysregulated Zn2+ homeostasis disrupts this axis to drive intervertebral disc degeneration.
Yuxin Jin, Yongcheng Chen, Yihao Xie, Dongyang Hu, Longhui Gong, Zhihua Chen, Linjie Chen, Minhao Gao, Qizhu Chen, Morgan Jones, Hui Wang, Xiuling You, Kenny Yat Hong Kwan, Shoutao Weng, Taidong Lyu, Xiang Chen, Yuxiao Zhu, Yusheng Wang, Bin Li, Xiangyang Wang, Xiaofeng Jia, Kailiang Zhou, Ouqiang Wu, Aimin Wu   +23 more
wiley   +1 more source

Salvianolic Acid a Disrupts the HSP90α‐AKT‐PERK Ternary Complex to Alleviate Atherosclerosis by Activating Endoplasmic Reticulum Stress of Senescent Vascular Smooth Muscle Cells

Advanced Science, EarlyView.
Salvianolic acid A (SAA) selectively induces senescent vascular smooth muscle cell apoptosis, alleviating atherosclerosis. Mechanistically, SAA targets heat shock protein 90 alpha (HSP90α) to disrupt the protein inase B (AKT)‐PRKR‐Like Endoplasmic Reticulum Kinase (PERK) scaffold, driving ubiquitin‐proteasome‐dependent AKT degradation.
Xiuya Guan, Mengkai Lu, Xinhai Cui, Dawei Zhang, Lei Zhang, Lin Lin, Yuan Li, Yuanlong Hu, Chao Li   +8 more
wiley   +1 more source

α-Galactosidase of Aspergillus niger: purification and properties [PDF]

, 2007
Highly purified α-galactosidase (specific activiys 25 U/mg protein) has been isolated from the cultural liquid of Aspergillus niger. The enzyme is thermo- and pH-stable, with pH-optimum 4.1, and temperature optimum 60 °C.
L. D. Varbanets, N. V. Borzova
core   +1 more source

Restores Aβ Clearance by Overcoming PCSK9–LRP1 Dysregulation and TRIB3‐Mediated Autophagy Blockade in Alzheimer's Disease

Advanced Science, EarlyView.
PCSK9 upregulation in cerebrovascular endothelial cells and microglia destabilizes the Aβ transporter LRP1, while TRIB3‐associated autophagy blockade further limits intracellular Aβ degradation. A RAP‐modified tea‐polyphenol nanoparticle co‐delivers siPCSK9 and siTRIB3 to restore LRP1‐mediated Aβ efflux and autophagy‐dependent degradation, reducing Aβ ...
Jie Miao, Jing Wang, Yankun Li, Haipei Zhang, Yanli Zhang, Qingnian Li, Bo Xiao, Wenhu Zhou, Junhong Guo   +8 more
wiley   +1 more source

Kinetics of soluble carbohydrates by action of endo/exo α-galactosidase enzyme in lentils and peas

, 2016
The kinetics of soluble sugars (available sugars and α-galactosides) during the action of naturally occurring endogenous α-galactosidase for up to 90 min in lentil and pea flours has been studied, and it has been compared with the addition of commercial ...
Doblado, Rosa, Vidal-Valverde, Concepción, Frías, Juana   +2 more
core   +1 more source

Immunohistochemical characterization of transgenic mice highly expressing human lysosomal α-galactosidase [PDF]

, 1998
Human lysosomal α-galactosidase predominantly hydrolyzes ceramide trihexoside. A transgenic mouse line, C57BL/6CrSIc–TgN(GLA) 1951 Rin, highly expressing human α-galactosidase, has been established and investigated biochemically and immunohistochemically
Shimmoto, Michie, Masaharu Kotani, Itoh, Kohji, Kase, Ryoichi, Kohji Itoh, Hiromichi Yonekawa, Kotani, Masaharu, Michie Shimmoto, Kouichi Utsumi, Utsumi, Kouichi, Taya, Choji, Choji Taya, Ryoichi Kase, Sakuraba, Hitoshi, Yonekawa, Hiromichi, Hitoshi Sakuraba   +15 more
core   +1 more source