Results 41 to 50 of about 937,878 (172)

The CXCL5/CXCR2 Axis Attenuates Ferroptosis in Intrahepatic Cholangiocarcinoma Through the Positive Feedback Loop Between PTGS2 Transcriptional Activation and Neutrophil Recruitment

Advanced Science, EarlyView.
The CXCL5/CXCR2 axis directly inhibits ferroptosis in ICC cells by activating the NF‐κB/PTGS2 pathway. Meanwhile, the CXCL5/CXCR2 axis recruits N2 TANs, and the activated PTGS2 promotes the secretion of TNF‐α from the recruited N2 TANs via the CCL2/CCL7/CCR2 pathway, thereby activating the NF‐κB/PTGS2 axis and forming a positive feedback loop, which ...
Chanqi Ye, Kefeng Lin, Shuyao Yang, Yan Zhang, Wenli Gao, Jiaqi Chen, Ying Dong, Rui Bai, Yang Tian, Minghao Wang, Zhijun Yuan, Yuxiao Ma, Yuqin He, Ruyin Chen, Wenguang Fu, Qi Jiang, Qiong Li, Jian Ruan   +17 more
wiley   +1 more source

MOESM4 of Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

, 2016
Additional file 4. Trends of the MID of intracellular free amino acids during 13C labeling experiments on Glc and on Glc/Glu.
Liu, Ping, Huang, Mingzhi, Guo, Menglei, Qian, Jiangchao, Lin, Weilu, Chu, Ju, Zhuang, Yingping, Zhang, Siliang   +7 more
openaire   +1 more source

A Microenvironment‐Adaptive Scaffold Rejuvenates Peripheral Nerve Regeneration During Aging Via Dual Modulation of Ferroptotic Stimuli in Schwann Cells

Advanced Science, EarlyView.
(i) Aging impairs peripheral nerve regeneration via its dual ferroptosis‐mediated pathologies in senescent schwann cells. (ii) The composite nerve conduit is a symbiosis niche integrating microenvironment‐responsive nanoparticles (Ga‐PTAs) and maxillofacial‐derived mesenchymal stem cells (Mmscs).
Ning Zhan, Lixin Tian, Lianjie Peng, Jingyu Zhang, Lining Lin, Xiaoting Zhang, Yaxian Luo, Zhichao Liu, Xinhua Yao, Mouyuan Sun, Yong He, Mengfei Yu, Huiming Wang   +12 more
wiley   +1 more source

Expression and characterization of recombinant α-galactosidase from taro

, 2014
碩士α-半乳糖水解酵素（α-galactosidase）是一種可以將非還原端半乳糖水解的酵素，在各種生物體中都存在，並且擁有許多不同的生物功能，例如在植物中α-半乳糖水解酵素參與醣類的代謝和運輸。在應用方面，α-半乳糖水解酵素可以使B型紅血球表面抗原上的半乳糖水解而變成O型紅血球，因此藉由基因工程的方法取得基因並利用 Pichia pastoris 的蛋白質表現系統來產生大量的芋頭α-半乳糖水解酵素以進行血型轉換的測試和應用。 本研究將已轉殖入α-半乳糖水解酵素基因的酵母菌大量培養 ...
陳逸書; Chen, I-Su
core   +1 more source

Taro α-galactosidase: A new gene product for blood conversion

, 2012
The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed ...
Chern, Ming-kai; Chien, Su-fang, Chern, Ming-kai; Li, Huang-yi; Chen, Po-fan; Chien, Su-fang   +1 more
core   +1 more source

In Vivo Direct Reprogramming: Current Progress and Future Prospects from Mechanisms to Therapeutic Application

Advanced Science, EarlyView.
This review examines the potential of in vivo direct reprogramming in regenerative medicine for functional tissue restoration, highlighting the role of tissue‐resident cues in generating functionally mature reprogrammed cells from lineage‐related cells. It contains a discussion on mechanisms, reprogramming factors, delivery approaches, and applications
Rishabh Deo Singh, Mauro Calvoli, Kyeong Kyu Kim   +2 more
wiley   +1 more source

Expression and characterization of recombinant α-galactosidase from rice

, 2010
碩士半乳糖水解酶可分解多醣，水解B型紅血球表面抗原的半乳糖基，使B型紅血球轉換成O型紅血球，可用於臨床輸血。本研究將稻米半乳糖水解酶基因整合到pPIC9K質體，在轉殖到重組酵母菌(Pichia pastoris)的染色體DAN上，並以4 mg/ml G418篩選出具有multicopy的菌株，以表現稻米半乳糖水解酶。重組酵母菌之培養條件測試，分別針對培養基種類、誘導溫度、誘導起始A600值、每日甲醇量及時間間隔、誘導培養基添加額外碳源檢測其對產量的影響，得到最佳表現量，細胞內酵素單位為5.124 ...
陳詩慧; Chen, Shi-hui
core   +1 more source

A rapid permeabilization procedure for accurate quantitative determination of β-galactosidase activity in yeast cells [PDF]

FEMS Microbiology Letters, 1995
A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or ...
openaire   +2 more sources

Impaired Chaperone‐Mediated Autophagy Accelerates Intervertebral Disc Degeneration by Inducing MIDN Accumulation to Target TSC2 for Proteasomal Degradation

Advanced Science, EarlyView.
This graphical abstract illustrates how chaperone‐mediated autophagy (CMA) regulates intervertebral disc degeneration (IDD). Under normal homeostasis (B), CMA degrades cytoplasmic Midnolin (MIDN) to maintain proteostasis. Under inflammatory stress (A), impaired CMA leads to cytoplasmic MIDN accumulation.
Xianglong Chen, Haiyang Gao, Wang Wu, Pengzhi Shi, Yuhang Chen, Anran Zhang, Zhangrong Cheng, Wenbo Wu, Zimu Yu, Yukun Zhang   +9 more
wiley   +1 more source

Gene cloning and expression of taro α-galactosidase

, 2010
碩士α-半乳糖水解酶（α-galactosidase）是一種可以將末端半乳糖水解的酵素，在各種生物體中都存在，並且擁有許多不同的生物功能，例如在植物中α-半乳糖水解酶參與醣類的代謝和運輸（source to sink transport）。在應用方面，α-半乳糖水解酶可以使B型紅血球表面抗原上的半乳糖水解而變成O型紅血球，因此藉由基因工程的方法取得基因並利用 Pichia pastoris 的蛋白質表現系統來產生大量的芋頭 α-半乳糖水解酶以進行血行轉換的測試和應用 ...
李皇毅; Lee, Houng-yi
core   +1 more source