Results 41 to 50 of about 57,026 (146)

Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]

FEMS Microbiology Letters, 1994
Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
openaire   +2 more sources

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
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Production of a Highly Protease-Resistant Fungal α-Galactosidase in Transgenic Maize Seeds for Simplified Feed Processing. [PDF]

PLoS ONE, 2015
Raffinose-family oligosaccharide (RFO) in soybeans is one of the major anti-nutritional factors for poultry and livestocks. α-Galactosidase is commonly supplemented into the animal feed to hydrolyze α-1,6-galactosidic bonds on the RFOs.
Wenxia Yang, Yuhong Zhang, Xiaojin Zhou, Wei Zhang, Xiaolu Xu, Rumei Chen, Qingchang Meng, Jianhua Yuan, Peilong Yang, Bin Yao   +9 more
doaj   +1 more source

Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]

Arthropods, 2020
The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei, Mohammad Ghadamyari, Sohrab Imani, et al.   +2 more
doaj  

Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects [PDF]

FEMS Microbiology Ecology, 2005
Previous studies suggest that, besides the maldigestion of lactose in the small intestine, the colonic processing of lactose might play a role in lactose intolerance. beta-Galactosidase is the bacterial enzyme which catalyzes the first step of lactose fermentation in the colon.
Tao, H, Priebe, MG, Vonk, RJ, Welling, GW   +3 more
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Isolation of Carbohydrate Hydrolyzing Enzymes from Red Kidney Beans [PDF]

Journal of Agricultural Chemistry and Biotechnology
Humans and other monogastric animals lack certain glycosidase enzymes that are widely present in plant seeds and animal tissues. This   study aimed to extract glycosidase enzymes from red kidney bean seeds.
Nancy Taha
doaj   +1 more source

Improving the Secretory Expression of an -Galactosidase from Aspergillus niger in Pichia pastoris. [PDF]

PLoS ONE, 2016
α-Galactosidases are broadly used in feed, food, chemical, pulp, and pharmaceutical industries. However, there lacks a satisfactory microbial cell factory that is able to produce α-galactosidases efficiently and cost-effectively to date, which prevents ...
Xianliang Zheng, Bo Fang, Dongfei Han, Wenxia Yang, Feifei Qi, Hui Chen, Shengying Li   +6 more
doaj   +1 more source

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
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Enzymatic reduction of galactooligosaccharide content of faba bean and yellow pea ingredients and food products

Future Foods, 2021
Intake of Fermentable Oligo,- Di,- Monosaccharides, And Polyols, abbreviated as FODMAP, is known to worsen gastrointestinal symptoms in people with functional bowel disorders.
Antti Nyyssölä, Anni Nisov, Martina Lille, Markus Nikinmaa, Natalia Rosa-Sibakov, Simo Ellilä, Mari Valkonen, Emilia Nordlund   +7 more
doaj   +1 more source

Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Microbial Cell Factories, 2021
Background The spore-forming lactic acid bacterium Bacillus coagulans MA-13 has been isolated from canned beans manufacturing and successfully employed for the sustainable production of lactic acid from lignocellulosic biomass. Among lactic acid bacteria,
Martina Aulitto, Andrea Strazzulli, Ferdinando Sansone, Flora Cozzolino, Maria Monti, Marco Moracci, Gabriella Fiorentino, Danila Limauro, Simonetta Bartolucci, Patrizia Contursi   +9 more
doaj   +1 more source