Results 51 to 60 of about 17,027 (139)

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Synaptic branch stability is mediated by non-enzymatic functions of MEC-17/αTAT1 and ATAT-2

Scientific Reports, 2022
Microtubules are fundamental elements of neuronal structure and function. They are dynamic structures formed from protofilament chains of α- and β-tubulin heterodimers.
Jean-Sébastien Teoh, Amruta Vasudevan, Wenyue Wang, Samiksha Dhananjay, Gursimran Chandhok, Roger Pocock, Sandhya P. Koushika, Brent Neumann   +7 more
doaj   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Ацетилювання α-тубуліну впливає на зміни білкового мікрооточення мікротрубочок при розвитку аутофагії в клітинах тютюну

Фактори експериментальної еволюції організмів, 2015
Aims. Microtubules (MTs) play an important role in the regulation of autophagy development in yeast and animal cells. MTs participate in maturation and traffic of autophagosomes through their dynamic state changes and post-translational modifications of
Д. І. Литвин, В. Д. Федина, А. І. Ємець, Я. Б. Блюм   +3 more
doaj  

Expression and localization of alpha-tubulin N-acetyltransferase 1 in the reproductive system of male mice

The Journal of Reproduction and Development, 2020
The structure of microtubules is essential for the fertilizing ability of spermatozoa. Acetylation of α-tubulin plays an important role in flagellar elongation and spermatozoa motility.
Rin YANAI, Yudai YAMASHITA, Kohei UMEZU, Yuuki HIRADATE, Kenshiro HARA, Kentaro TANEMURA   +5 more
doaj   +1 more source

LukS-PV inhibits hepatocellular carcinoma cells migration by downregulating HDAC6 expression

BMC Cancer, 2022
Background Hepatocellular carcinoma (HCC) is a clinically common malignant tumor worldwide. LukS-PV is the S component of Panton-Valentine leukocidin secreted by Staphylococcus aureus, which has shown anti-cancer activity.
Xuexue Xu, Pengsheng Ding, Lan Shi, Gang Wu, Xiaoling Ma   +4 more
doaj   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Analysis of the role of acetylation in Giardia lamblia and the giardicidal potential of garcinol

Frontiers in Microbiology
IntroductionPost-translational modifications of proteins provide cellular physiology with a broad range of adaptability to the external environment flexibly and rapidly.
Rocío G. Patolsky, Jerónimo Laiolo, Jerónimo Laiolo, Luciano Díaz-Pérez, Gabriel Luna Pizarro, Gonzalo F. Mayol, María C. Touz, Constanza Feliziani, Andrea S. Rópolo   +8 more
doaj   +1 more source

p27Kip1 Modulates Axonal Transport by Regulating α-Tubulin Acetyltransferase 1 Stability

Cell Reports, 2018
Summary: The protein p27Kip1 plays roles that extend beyond cell-cycle regulation during cerebral cortex development, such as the regulation of neuronal migration and neurite branching via signaling pathways that converge on the actin and microtubule ...
Giovanni Morelli, Aviel Even, Ivan Gladwyn-Ng, Romain Le Bail, Michal Shilian, Juliette D. Godin, Elise Peyre, Bassem A. Hassan, Arnaud Besson, Jean-Michel Rigo, Miguel Weil, Bert Brône, Laurent Nguyen   +12 more
doaj   +1 more source

The regulation of stem cell fate and its application in neural regeneration

Interdisciplinary Medicine, EarlyView.
Regulating stem cell fate is crucial for neural regeneration. This review summarizes key physical, biological, and chemical strategies and their applications in repairing nerve injuries, providing new insights for regenerative medicine. Abstract Regulating the fate of stem cells (SCs) is a key technical problem in the field of regenerative medicine and
Yuexin He, Xingyu Chen, Minghong He, Zhe Li   +3 more
wiley   +1 more source