Results 11 to 20 of about 132,836 (234)

Thermostable α-galactosidase fromBacillus stearothermophilusNUB3621: cloning, sequencing and characterization [PDF]

FEMS Microbiology Letters, 1999
An alpha-galactosidase gene from the thermophilic bacterium Bacillus stearothermophilus NUB3621 was cloned, sequenced, expressed in Escherichia coli and the recombinant protein was purified. The Bacillus enzyme, designated AgaN, is similar to alpha-galactosidases of family 36 in the classification of glycosyl hydrolases.
Hildegard Watzlawick, Axel Gehweiler, Olafur Fridjonsson, Ralf Mattes   +3 more
openaire   +3 more sources

High-level expression of Aspergillus niger β-galactosidase in Ashbya gossypii [PDF]

, 2014
Ashbya gossypii has been recently considered as a host for the expression of recombinant proteins. The production levels achieved thus far were similar to those obtained with Saccharomyces cerevisiae for the same proteins.
Aguiar, Ashby, Dietrich, Domingues, Domingues, Domingues, Dünkler, Gietz, Gonçalves, Gueldener, Jiménez, Jiménez, Kato, Kaufmann, Knechtle, Kumar, Manzanares, Monschau, Nevalainen, Nevalainen, O'Connell, Oliveira, Oliveira, Park, Pompejus, Ramakrishnan, Revuelta Doval, Ribeiro, Ribeiro, Ribeiro, Ruohonen, Stahmann, Steiner, Steiner, Tajima, Wendland, Wickerham, Wright   +37 more
core   +1 more source

A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei [PDF]

, 1992
Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but
Alsner, Altschul, Ascoli, Beck, Bensch, Bestor, Bestor, Bestor, Bestor, Bestor, Brancolini, Bravo, Carmo-Fonseca, Carter, Cook, Czank, D'Andrea, Fox, Fu, Gauthier-Rouvière, Goldman, Gruenbaum, Hatton, Huang, Kaiser, Kambach, Kumar, Lasko, Lauster, Lawrence, Li, Li, Matthias, Meier, Mills, Morris, Nakamura, Nakayasu, Noguchi, O'Keefe, Ottiger, Peculis, Posfai, Roise, Sambrook, Saunders, Spector, Tomkinson, Tubo, Vissel, Wang, Weier   +51 more
core   +1 more source

Anaerobic expression of thegyrAgene demonstrated by β-galactosidase activity ingyrA::Mu-lacfusion derivatives ofEscherichia coli [PDF]

FEMS Microbiology Letters, 1988
E. coli C600Δ lac was infected with a Mu- lac defective phage. Lysogens resistant to ampicillin (25 µg/ml) and nalidixic acid (20 µg/ml) were unable to grow anaerobically and insensitive to nalidixic acid (150 µg/ml), indicating formation of gyrA ::Mu- lac fusions.
Mary L. Droffner, Nobuto Yamamoto
openaire   +2 more sources

High lysosomal activities in cystic fibrosis tracheal gland cells corrected by adenovirus-mediated CFTR gene transfer [PDF]

, 1999
Human tracheal gland serous (HTGS) cells are now believed to be a major target of cystic fibrosis (CF) gene therapy. To evaluate the efficiency of adenovirus-mediated gene transfer in these cells we tested the adenovirus construction containing β ...
Bianco, N., Figarella, C., Kammouni, W., Merten, M.D., Naı̈mi, D., Renaud, W.   +5 more
core   +1 more source

Expression of β-galactosidase inRhizobium japonicum [PDF]

FEMS Microbiology Letters, 1985
The plasmid pGC91.14 was used to introduce via conjugation the Escherichia coli lac operon into fast-growing and slow-growing strains of Rhizobium japonicum. Exconjugants now expressed higher levels of β-galactosidase activity which was still inducible by isopropyl-β-d-thiogalactoside (IPTG).
openaire   +2 more sources

Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger β-galactosidase production [PDF]

, 2007
A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration
Domingues, Lucília, Lima, Nelson, Oliveira, Carla Cristina Marques de, Silva, Nancy A. da, Teixeira, J. A.   +4 more
core   +1 more source

Antigenicity of a viral peptide displayed on β-galactosidase fusion proteins is influenced by the presence of the homologous partner protein [PDF]

FEMS Microbiology Letters, 1996
Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competitive ELISA using a panel of seven ...
Antonio Villaverde, José Luis Corchero
openaire   +3 more sources

Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs

Animal Research and One Health, EarlyView., 2023
In the present study, we created the weight gain models with Min and Yorkshire pigs and conducted the metagenomics and metabolomics of colon contents and transcriptomics of colon tissue. Further, we revealed the characteristics and functions of the colonic microbiota in Min and Yorkshire pigs and analyzed their interactions with the host. Overall, this
Xiaoyu Huang, Huihui Li, Feng Cheng, Ligang Wang, Guoqing Cao, Lixian Wang, Lijun Shi   +6 more
wiley   +1 more source

Immobilization of b-galactosidase from kluyveromyces lactis onto a polysiloxane–polyvinyl alcohol magnetic (mPOS–PVA) composite for lactose hydrolysis [PDF]

, 2008
β-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS–PVA, using glutaraldehyde as activating agent and its properties were evaluated.
Balcão, V. M., Carneiro-da-Cunha, Maria G., Carvalho Junior, L. B., Neri, D. F. M., Teixeira, J. A.   +4 more
core   +1 more source