Effect of mechanical denaturation on surface free energy of protein powders [PDF]
, 2016 yesGlobular proteins are important both as therapeutic agents and excipients. However, their fragile native conformations can be denatured during pharmaceutical processing, which leads to modification of the surface energy of their powders and hence ...
Blagbrough, I.S. +4 more
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Bioactive Materials, 2021 We report on the fabrication and characterization of color-encoded chitosan hydrogels for the rapid, sensitive and specific detection of bacterial enzymes as well as the selective detection of a set of tested bacteria through characteristic enzyme ...
Zhiyuan Jia +7 more
doaj +1 more source
Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells [PDF]
, 2012 The adaptive immune response to viral vectors reduces vector-mediated transgene expression from the brain. It is unknown, however, whether this loss is caused by functional downregulation of transgene expression or death of transduced cells.
Ahmed +60 more
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Senescence Associated β-galactosidase Staining
Bio-Protocol, 2012 Detection of senescent cells using a cytochemical assay was first described in 1995 (Dimri et al., 1995). The identification of senescent cells is based on an increased level of lysosomal β-galactosidase activity (Kurz et al., 2000).
Michael Eccles, Caiyun Li
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Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system. [PDF]
PLoS ONE, 2011 Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated
Xiaodong Mu +4 more
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Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]
FEMS Microbiology Letters, 1994 Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
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Tetrahedron, 2013 Synthesis of N-acetyl-D-lactosamine (Gal-β[1→4]GlcNAc, LacNAc) catalyzed by β-galactosidase from Thermus thermophilus (TTP0042) is affected by side reactions that give as result very low yields (about 20%) of LAcNAc when the reaction is performed in buffer.
Sandoval, Manuel +4 more
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The zinc cluster proteins Upc2 and Ecm22 promote filamentation in Saccharomyces cerevisiae by sterol biosynthesis-dependent and -independent pathways. [PDF]
, 2015 The transition between a unicellular yeast form to multicellular filaments is crucial for budding yeast foraging and the pathogenesis of many fungal pathogens such as Candida albicans.
Höfken, T, Woods, K
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Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]
Arthropods, 2020 The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei +2 more
doaj
Axitinib induces senescence-associated cell death and necrosis in glioma cell lines: The proteasome inhibitor, bortezomib, potentiates axitinib-induced cytotoxicity in a p21(Waf/Cip1) dependent manner. [PDF]
, 2017 Glioblastoma is associated with a poor overall survival despite new treatment advances. Antiangiogenic strategies targeting VEGF based on tyrosine kinase inhibitors (TKIs) are currently undergoing extensive research for the treatment of glioma.
Amantini, C +7 more
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