Results 51 to 60 of about 132,836 (234)

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

Protein aggregation and neurodegenerative disease: Structural outlook for the novel therapeutics

Proteins: Structure, Function, and Bioinformatics, EarlyView., 2023
Abstract Before the controversial approval of humanized monoclonal antibody lecanemab, which binds to the soluble amyloid‐β protofibrils, all the treatments available earlier, for Alzheimer's disease (AD) were symptomatic. The researchers are still struggling to find a breakthrough in AD therapeutic medicine, which is partially attributable to lack in ...
Sharif Arar, Md Anzarul Haque, Rakez Kayed   +2 more
wiley   +1 more source

MOESM4 of Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

, 2016
Additional file 4. Trends of the MID of intracellular free amino acids during 13C labeling experiments on Glc and on Glc/Glu.
Liu, Ping, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Chu, Ju, Yingping Zhuang, Siliang Zhang   +7 more
openaire   +1 more source

Picomolar inhibition of β-galactosidase (bovine liver) attributed to loop closure [PDF]

Bioorganic & Medicinal Chemistry, 2017
In an effort to examine similarities in the active sites of glycosidases within the GH35 family, we performed a structure-activity-relationship study using our recently described library of galactonoamidines. The kinetic evaluation based on UV/Vis spectroscopy disclosed inhibition of β-galactosidase (bovine liver) in the picomolar concentration range ...
Pickens, Jessica, Wang, Feng, Striegler, Susanne   +2 more
openaire   +3 more sources

Antioxidant and Anti‐Senescence Polyvinyl Alcohol‐Gallic Acid Supramolecular Hydrogels for Stem Cell Culture

Advanced Healthcare Materials, EarlyView.
The PVA‐GA hydrogel is formed through hydrogen bonding between PVA hydroxyl groups and GA galloyl units, enabling sustainable GA release and its extended biological activity. The hydrogel scavenges ROS to reduce oxidative stress, preventing mitochondrial damage from excessive ROS, mitigating hADSC senescence, and preserving VEGF secretion, offering a ...
Yiduo Zhou, Matías L. Picchio, Yan Nie, Lei Wang, Oihane Sanz, Yue Liu, Xun Xu, Lukas Prantl, Oliver Felthaus, Weiwei Wang, Marcelo Calderón, Nan Ma   +11 more
wiley   +1 more source

Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells [PDF]

, 2012
The adaptive immune response to viral vectors reduces vector-mediated transgene expression from the brain. It is unknown, however, whether this loss is caused by functional downregulation of transgene expression or death of transduced cells.
Ahmed, Ali, Asadi-Moghaddam, Bantug, Barcia, Barcia, Barcia, Barcia, Barcia, Barcia, Bergmann, Byrnes, Byrnes, Carlos Barcia, Chen, Chiocca, Chunyan Liu, Conrady, Dewey, Diamond, Glass, Griffin, Griffin, Hausmann, Huse, Jeffrey M Zirger, Jiang, Jiang, Jiang, Josee Bergeron, Kanegae, Kurt M Kroeger, Lang, Lowenstein, Lowenstein, Maria G Castro, Mariana Puntel, McGavern, Mia Wibowo, Määttä, Niyati Bondale, O'Keefe, Parra, Paxinos, Pedro R Lowenstein, Pulkkanen, Puntel, Rall, Rameen Moridzadeh, Shi, Smith-Arica, Southgate, Southgate, Thomas, Thomas, Wang, Wilkinson, Williamson, Wirth, Yang, Zambrowicz   +60 more
core   +1 more source

Examination of a blood-brain barrier targeting β-galactosidase-monoclonal antibody fusion protein in a murine model of GM1-gangliosidosis

Molecular Genetics and Metabolism, 2021
GM1-gangliosidosis is a lysosomal disease resulting from a deficiency in the hydrolase β-galactosidase (β-gal) and subsequent accumulation of gangliosides, primarily in neuronal tissue, leading to progressive neurological deterioration and eventually early death.
Przybilla, Michael J, Stewart, Christine, Carlson, Timothy W, Ou, Li, Koniar, Brenda L, Sidhu, Rohini, Kell, Pamela J, Jiang, Xuntian, Jarnes, Jeanine R, O'Sullivan, M Gerard, Whitley, Chester B   +10 more
openaire   +6 more sources

Liquid Crystalline Networks Hamper the Malignancy of Cancer Cells

Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.
Liquid Crystalline Networks are used as scaffolds for the growth of A375 melanoma cells demonstrating to affect their malignancy. Indeed, only by contact, these materials reduces cell proliferation and colony formation capacity, while increasing the number of senescent cells and promoting the mesenchymal to epithelial transition.
Daniele Martella, Ignazia Tusa, Alessandro Tubita, Alessia Negri, Marco Turriani, Marta Rojas‐Rodríguez, Martina Salzano de Luna, Alessio Menconi, Camilla Parmeggiani, Elisabetta Rovida   +9 more
wiley   +1 more source

Simultaneous imaging of a lacZ-marked tumor and microvasculature morphology in vivo by dual-wavelength photoacoustic microscopy [PDF]

, 2008
Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a ...
Li, Li, Maslov, Konstantin, Wang, Lihong V., Zemp, Roger J., Zhang, Hao F.   +4 more
core   +2 more sources

Engineering the Future of Restorative Clinical Peripheral Nerve Surgery

Advanced Healthcare Materials, EarlyView.
What if damaged nerves could regenerate more effectively? This review unveils cutting‐edge strategies to restore nerve function, from biomaterial scaffolds and bioactive molecules to living engineered tissues. By accelerating axonal regrowth, preserving Schwann cells, and enhancing connectivity, these approaches are reshaping nerve repair—offering new ...
Justin C. Burrell, Zarina S. Ali, Eric L. Zager, Joseph M. Rosen, Mykhailo M. Tatarchuk, D. Kacy Cullen   +5 more
wiley   +1 more source