Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation [PDF]
Marine Drugs, 2021 β-galactosidases (EC 3.2.1.23) catalyze the hydrolysis of β-galactosidic bonds in oligosaccharides and, under certain conditions, transfer a sugar moiety from a glycosyl donor to an acceptor.
Marco Mangiagalli, Marina Lotti
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Infant-Associated Bifidobacterial β-Galactosidases and Their Ability to Synthesize Galacto-Oligosaccharides [PDF]
Frontiers in Microbiology, 2021 Galacto-oligosaccharides (GOS) represent non-digestible glycans that are commercially produced by transgalactosylation of lactose, and that are widely used as functional food ingredients in prebiotic formulations, in particular in infant nutrition.
Valentina Ambrogi +14 more
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Characterization of two β-galactosidases LacZ and WspA1 from Nostoc flagelliforme with focus on the latter’s central active region [PDF]
Scientific Reports, 2021 The identification and characterization of new β-galactosidases will provide diverse candidate enzymes for use in food processing industry. In this study, two β-galactosidases, Nf-LacZ and WspA1, from the terrestrial cyanobacterium Nostoc flagelliforme ...
Xiang Gao +5 more
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β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization [PDF]
Microorganisms, 2020 Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume produced and the high ...
María Florencia Eberhardt +2 more
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Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates [PDF]
AMB Express, 2019 Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize ...
Valentina Ambrogi +7 more
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Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences. [PDF]
PLoS ONE, 2017 The techniques of metagenomics have allowed researchers to access the genomic potential of uncultivated microbes, but there remain significant barriers to determination of gene function based on DNA sequence alone.
Jiujun Cheng +6 more
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Intrinsic dynamic behavior of enzyme:substrate complexes govern the catalytic action of β-galactosidases across clan GH-A [PDF]
Scientific Reports, 2019 The conformational itineraries taken by carbohydrate residues in the catalytic subsite of retaining glycoside hydrolases (GHs), harness the link between substrate conformation and reactivity.
Rajender Kumar +2 more
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Identification and characterization of a novel β-galactosidase active at low temperatures from the Antarctic fungus Tetracladium sp., expressed in Saccharomyces cerevisiae [PDF]
Microbial Cell FactoriesBackground β-Galactosidases are widely used in the dairy industry to produce lactose-free milk and prebiotics such as galacto-oligosaccharides and lactulose.
Fernando Gutierrez +3 more
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Genome-Wide Analysis of β-Galactosidases in Xanthomonas campestris pv. campestris 8004 [PDF]
Frontiers in Microbiology, 2018 Bacterial β-galactosidase is involved in lactose metabolism and acts as a prevalent reporter enzyme used in studying the activities of prokaryotic and eukaryotic promoters. Xanthomonas campestris pv.
Huiqi Wang +13 more
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Mechanism-Based Allylic Carbasugar Chlorides That Form Covalent Intermediates with α- and β-Galactosidases [PDF]
MoleculesGlycoside hydrolases have been implicated in a wide range of human conditions including lysosomal storage diseases. Consequently, many researchers have directed their efforts towards identifying new classes of glycoside hydrolase inhibitors, both ...
Oluwafemi Akintola +2 more
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