Results 21 to 30 of about 2,910 (220)

Yersinia pestis lacZexpresses a β-galactosidase with low enzymatic activity [PDF]

FEMS Microbiology Letters, 2006
Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y.
Alexander G, Bobrov, Robert D, Perry
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Thermostable α-galactosidase fromBacillus stearothermophilusNUB3621: cloning, sequencing and characterization [PDF]

FEMS Microbiology Letters, 1999
An alpha-galactosidase gene from the thermophilic bacterium Bacillus stearothermophilus NUB3621 was cloned, sequenced, expressed in Escherichia coli and the recombinant protein was purified. The Bacillus enzyme, designated AgaN, is similar to alpha-galactosidases of family 36 in the classification of glycosyl hydrolases.
O, Fridjonsson, H, Watzlawick, A, Gehweiler, R, Mattes   +3 more
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Biochemical characterization of three Aspergillus niger β-galactosidases

Electronic Journal of Biotechnology, 2017
Background: β-Galactosidases catalyze both hydrolytic and transgalactosylation reactions and therefore have many applications in food, medical, and biotechnological fields.
Dandan Niu, Xiaojing Tian, Nokuthula Peace Mchunu, Chao Jia, Suren Singh, Xiaoguang Liu, Bernard A. Prior, Fuping Lu   +7 more
doaj   +1 more source

Expression of β-galactosidase inRhizobium japonicum [PDF]

FEMS Microbiology Letters, 1985
The plasmid pGC91.14 was used to introduce via conjugation the Escherichia coli lac operon into fast-growing and slow-growing strains of Rhizobium japonicum. Exconjugants now expressed higher levels of β-galactosidase activity which was still inducible by isopropyl-β-d-thiogalactoside (IPTG).
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Transcriptional analysis of the β-galactosidase gene (pbg) inClostridium perfringens [PDF]

FEMS Microbiology Letters, 1995
The mode of expression of the beta-galactosidase gene (pbg) of Clostridium perfringens was examined. The pbg gene was transcribed on a single 3.7-kb mRNA. The transcript contained a message for ORF54, located upstream of the pbg gene in the chromosome, indicating that ORF54 and the pbg gene comprise one operon (pbg operon). Expression of the pbg operon
T, Kobayashi, T, Shimizu, H, Hayashi
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Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

Biotechnology for Biofuels, 2021
Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as ...
Zhi-Peng Wang, Xin-Yue Zhang, Yan Ma, Jing-Run Ye, Jing Jiang, Hai-Ying Wang, Wei Chen   +6 more
doaj   +1 more source

β-Galactosidase-Producing Isolates in Mucoromycota: Screening, Enzyme Production, and Applications for Functional Oligosaccharide Synthesis

Journal of Fungi, 2021
β-Galactosidases of Mucoromycota are rarely studied, although this group of filamentous fungi is an excellent source of many industrial enzymes. In this study, 99 isolates from the genera Lichtheimia, Mortierella, Mucor, Rhizomucor, Rhizopus and ...
Bettina Volford, Mónika Varga, András Szekeres, Alexandra Kotogán, Gábor Nagy, Csaba Vágvölgyi, Tamás Papp, Miklós Takó   +7 more
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Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide)

Plant Protection Science, 2012
The red palm weevil, Rhynchophorus ferrugineus (Olivier), is one of the most destructive pests of palm trees in the southeast of Iran. Digestion in the alimentary canal of the red palm weevil is facilitated by some carbohydrases.
Nasir Saberi RISEH, Mohammad GHADAMYARI, Behnam MOTAMEDINIYA   +2 more
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Anaerobic expression of thegyrAgene demonstrated by β-galactosidase activity ingyrA::Mu-lacfusion derivatives ofEscherichia coli [PDF]

FEMS Microbiology Letters, 1988
E. coli C600Δ lac was infected with a Mu- lac defective phage. Lysogens resistant to ampicillin (25 µg/ml) and nalidixic acid (20 µg/ml) were unable to grow anaerobically and insensitive to nalidixic acid (150 µg/ml), indicating formation of gyrA ::Mu- lac fusions.
Nobuto Yamamoto, Mary L. Droffner
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Purification and molecular properties of an α-galactosidase synthesized and secreted byAspergillus nidulans [PDF]

FEMS Microbiology Letters, 1993
alpha-Galactosidases from mycelial extract and culture filtrate of Aspergillus nidulans have been purified to homogeneity and utilised to obtain polyclonal antibodies anti-alpha-galactosidase. The enzymatic characteristics and the cross reactivity of the antibodies suggest that alpha-galactosidases isolated from the two sources were the same enzyme ...
S, Ríos, A M, Pedregosa, I, Fernández Monistrol, F, Laborda   +3 more
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