Results 31 to 40 of about 2,910 (220)

Cloning, Expression, Purification, and Characterization of β-Galactosidase from Bifidobacterium longum and Bifidobacterium pseudocatenulatum

Molecules, 2022
Expression and purification of β-galactosidases derived from Bifidobacterium provide a new resource for efficient lactose hydrolysis and lactose intolerance alleviation. Here, we cloned and expressed two β-galactosidases derived from Bifidobacterium. The
Mingzhu Du, Shuanghong Yang, Tong Jiang, Tingting Liang, Ying Li, Shuzhen Cai, Qingping Wu, Jumei Zhang, Wei Chen, Xinqiang Xie   +9 more
doaj   +1 more source

Experimental conditions may affect reproducibility of the beta-galactosidase assay [PDF]

FEMS Microbiology Letters, 1992
Several experimental conditions and parameters contributing to the determination of beta-galactosidase activity, as proposed in Miller's assay, were studied. Use of the absorbance correction factor and the nature and concentration of permeabilizing agents were taken into account as different experimental conditions.
GIACOMINI, ALESSIO, CORICH, VIVIANA, F. J. Ollero, SQUARTINI, ANDREA, M. P. Nuti   +4 more
openaire   +2 more sources

Cloning of the phospho-β-galactosidase gene inEscherichia colifrom lactose-negative mutants ofStreptococcus mutansisolated following random mutagenesis with plasmid pVA891 clone banks [PDF]

FEMS Microbiology Letters, 1992
In order to mutagenize Streptococcus mutans a marker rescue plasmid, pVA891, was employed. The plasmid was ligated with Sau3AI digested chromosomal DNA fragments from S. mutans GS-5IS3 and the resultant plasmids were amplified in Escherichia coli.
Yutaka Sato, Yasuhito Yamamoto, Harutoshi Kizaki, Howard K. Kuramitsu   +3 more
openaire   +1 more source

Selection of lactic acid bacteria strain for simultaneous production of α- and β-galactosidases

Zaštita Materijala, 2016
Galactosidases are group of enzymes gaining increasing attention in recent times due to the fact they catalyse hydrolysis of galatose-containing oligosaccharide, which reduce digestibility of various food and feed products.
Milica Carević, Katarina Banjanac, Marija Ćorović, Sonja Jakovetić, Ana Milivojević, Sekulić Maja Vukašinović, Dejan Bezbradica   +6 more
doaj   +1 more source

Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strainLactobacillus acidophilusR22 [PDF]

FEMS Microbiology Letters, 2007
Beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 was purified to apparent homogeneity by ammonium sulphate fractionation, hydrophobic interaction, and affinity chromatography. The enzyme is a heterodimer consisting of two subunits of 35 and 72 kDa, as determined by gel electrophoresis.
Thu-Ha, Nguyen, Barbara, Splechtna, Stanimira, Krasteva, Wolfgang, Kneifel, Klaus D, Kulbe, Christina, Divne, Dietmar, Haltrich   +6 more
openaire   +2 more sources

Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]

Arthropods, 2020
The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei, Mohammad Ghadamyari, Sohrab Imani, et al.   +2 more
doaj  

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
openaire   +2 more sources

Les polymères de mannose en production animale. 2. Les enzymes de dégradation des mannanes dans l’alimentation des porcs et des volailles

INRAE Productions Animales, 2021
Les enzymes capables de dégrader les mannanes appartiennent à plusieurs familles d’hydrolases qui se distinguent par les sites de coupure sur ces polysaccharides.
Estelle BONNIN, Michel LESSIRE, Nathaële WACRENIER, Fabien ALLEMAN   +3 more
doaj   +1 more source

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
openaire   +2 more sources

High gene expression inEscherichia coliof recombinant alginate lyase as a fused protein with β-galactosidase α-peptide [PDF]

FEMS Microbiology Letters, 1995
Escherichia coli LE392 (pAL28) was previously isolated as a positive clone harboring the alginate lyase gene (aly) from an alginate-degrading strain, Pseudomonas sp. OS-ALG-9. The plasmid pAL205, one of the constructs obtained after successive subcloning of pAL28, gave the highest expression of aly in E. coli cells.
K, Fujiyama, H, Maki, S, Kinoshita, T, Yoshida   +3 more
openaire   +2 more sources