Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes [PDF]
Applied and Environmental Microbiology, 2006 From near to far , from here to there , funny things are everywhere. —Dr. Seuss, One Fish Two Fish Red Fish Blue Fish In 1909, H. Joel Conn ([25][1]) expressed the hope that methods would soon be at hand by which the significance of the different bacteria present in any soil could ...
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Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]
, 2011 Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Bogert, B., van den +3 more
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Diversity of 16S rRNA Genes within Individual Prokaryotic Genomes [PDF]
Applied and Environmental Microbiology, 2010 ABSTRACT Analysis of intragenomic variation of 16S rRNA genes is a unique approach to examining the concept of ribosomal constraints on rRNA genes; the degree of variation is an important parameter to consider for estimation of the diversity of a complex microbiome in the recently initiated Human Microbiome Project ...
Anna Y, Pei +15 more
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Defining reference sequences for Nocardia species by similarity and clustering analyses of 16S rRNA gene sequence data. [PDF]
PLoS ONE, 2011 BACKGROUND: The intra- and inter-species genetic diversity of bacteria and the absence of 'reference', or the most representative, sequences of individual species present a significant challenge for sequence-based identification.
Manal Helal +5 more
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16S rRNA gene Library Preparation Protocol v1
, 2023 16S rRNA gene library preparation protocol including the following steps: MPure extraction of Bacterial DNA, Manual 16S indexed primer PCR, library clean up with beads, quibit DNA quantification, equimolar mix construction, agarose gel DNA concentration and gel clean up to produce a library ready for Illumina sequencing.
dinesh.aggarwal not provided +3 more
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PCR-generated artefact from 16S rRNA gene-specific primers [PDF]
FEMS Microbiology Letters, 2005 Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex ...
Catherine A, Osborne +3 more
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MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with skin disorders is important for dermatological advancement in both diagnosis and novel treatments. This study aims to analyze and improve the accuracy of taxonomic classification of skin bacteria with MinION ...
Miquel Rozas +4 more
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The complete mitochondrial genome of the house dust mite Dermatophagoides pteronyssinus (Trouessart): a novel gene arrangement among arthropods [PDF]
, 2009 Background: The apparent scarcity of available sequence data has greatly impeded evolutionary studies in Acari (mites and ticks). This subclass encompasses over 48,000 species and forms the largest group within the Arachnida.
Dermauw, Wannes +3 more
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BMC Microbiology, 2021 Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host ...
M. Singer +9 more
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Characterization of Francisella species isolated from the cooling water of an air conditioning system. [PDF]
, 2015 Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive.
Atwill, Edward +6 more
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