Streamlined Method to Analyze 16S rRNA Gene Clone Libraries [PDF]
BioTechniques, 2001 Kevin L. Vergin +2 more
doaj +3 more sources
Environmental Microbiome, 2020 Sequencing of 16S rRNA genes has become a powerful technique to study microbial communities and their responses towards changing environmental conditions in various ecosystems.
F. Wemheuer +6 more
semanticscholar +1 more source
Variable copy number, intra-genomic heterogeneities and lateral transfers of the 16S rRNA gene in Pseudomonas. [PDF]
PLoS ONE, 2012 Even though the 16S rRNA gene is the most commonly used taxonomic marker in microbial ecology, its poor resolution is still not fully understood at the intra-genus level.
Josselin Bodilis +3 more
doaj +1 more source
Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton. [PDF]
, 2018 Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats.
Carlson, Craig A +3 more
core +1 more source
Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences
Nature Biotechnology, 2013 Profiling phylogenetic marker genes, such as the 16S rRNA gene, is a key tool for studies of microbial communities but does not provide direct evidence of a community's functional capabilities.
M. Langille +11 more
semanticscholar +1 more source
Activity profiles for marine sponge-associated bacteria obtained by 16S rRNA vs 16S rRNA gene comparisons [PDF]
The ISME Journal, 2010 Abstract The phylogenetic diversity of microorganisms in marine sponges is becoming increasingly well described, yet relatively little is known about the activities of these symbionts. Given the seemingly favourable environment provided to microbes by their sponge hosts, as indicated by the extraordinarily high abundance of sponge ...
Janine, Kamke +2 more
openaire +2 more sources
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
, 2018 In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini +4 more
core +3 more sources
Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
Nature Communications, 2019 The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect.
Jethro S. Johnson +11 more
semanticscholar +1 more source
Nitrogen removal in a two-chambered microbial fuel cell: Establishment of a nitrifying-denitrifying microbial community on an intermittent aerated cathode [PDF]
, 2016 A microbial fuel cell (MFC) was used to study nitrogen dynamics and its feasibility for high strength wastewater treatment. Intermittent aeration was applied on the cathode chamber accomplishing the establishment of a simultaneous nitrifying-denitrifying
Bonmatí Blasi, August +3 more
core +2 more sources
Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]
, 2020 Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet +3 more
core +1 more source