Results 161 to 170 of about 88,507,977 (198)

Combinatorial Engineering of Escherichia coli for Enhancing 3-Fucosyllactose Production

ACS Synthetic Biology
3-Fucosyllactose (3-FL) is an important fucosylated human milk oligosaccharide (HMO) with biological functions such as promoting immunity and brain development. Therefore, the construction of microbial cell factories is a promising approach to synthesizing 3-FL from renewable feedstocks.
Huiyuan Huang, Wenwen Yu, Xianhao Xu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu   +7 more
openaire   +2 more sources

Combinatorial Optimization Strategies for 3-Fucosyllactose Hyperproduction in Escherichia coli

Journal of Agricultural and Food Chemistry
3-Fucosyllactose (3-FL), an important fucosylated human milk oligosaccharide in breast milk, offers numerous health benefits to infants. Previously, we metabolically engineered Escherichia coli BL21(DE3) for the in vivo biosynthesis of 3-FL. In this study, we initially optimized culture conditions to double 3-FL production.
Zhihui Du, Yingying Zhu, Zhen Lu, Roulin Chen, Zhaolin Huang, Yihan Chen, Cuie Guang, Wanmeng Mu   +7 more
openaire   +2 more sources

3-Fucosyllactose-mediated modulation of immune response against virus infection

International Journal of Antimicrobial Agents
Viral pathogens, particularly influenza and SARS-CoV-2, pose a significant global health challenge. Given the immunomodulatory properties of human milk oligosaccharides, in particular 2'-fucosyllactose and 3-fucosyllactose (3-FL), we investigated their dietary supplementation effects on antiviral responses in mouse models.
Seokoh Moon, Ki Wook Lee, Myungseo Park, Jeonghui Moon, Sang Hee Park, Soomin Kim, Jaehyeon Hwang, Jong-Won Yoon, Seon-Min Jeon, Jun-Seob Kim, Young-Jun Jeon, Dae-Hyuk Kweon   +11 more
openaire   +2 more sources

Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers

Bioresource Technology
Biosensor-based high-throughput screening is efficient for improving industrial microorganisms. There is a severe shortage of human milk oligosaccharides (HMOs) biosensors. This study established a 3-fucosyllactose (3-FL, a kind of HMOs) whole-cell biosensor by coupling cell growth with production.
Qinggang, Li, Chuan, Liu, Jinhuai, He, Tiantian, Liu, Wencong, Zhang, Zhenzhen, Xie, Jianfei, Zong, Yu, Li, Xue, Sun, Fuping, Lu   +9 more
openaire   +2 more sources

Escherichia coli immobilized fermentation for continuous 3-fucosyllactose production via manipulating biofilm formation

Bioresource Technology
3-Fucosyllactose (3-FL), a representative human milk oligosaccharide, is witnessing rapidly increasing demand. Establishing an immobilized fermentation system using microbial cell factories presents a promising strategy for continuous 3-FL production.
Jing, Leng, Weiqiang, Yang, Qingyuan, Yao, Daoguang, Zhu, Chaowei, Zhou, Ting, Xu, Huanqing, Niu, Pengpeng, Yang, Dong, Liu, Yong, Chen, Hanjie, Ying   +10 more
openaire   +2 more sources

Quantitative Nuclear Magnetic Analysis of Human Milk Oligosaccharides 2’-Fucosyllactose and 3-Fucosyllactose in Complicated Food Matrices

Food Chemistry
Human milk oligosaccharides (HMOs) are the third most abundant solid component in breast milk, which is essential for the healthy growth of infants. 2'-fucosyllactose (2'-FL) and 3-fucosyllactose (3-FL) are the main components of HMOs. In this study, we developed and validated a quantitative nuclear magnetic resonance (qNMR) method for determining HMOs
Di Feng, Qiding Zhong, Daobing Wang
exaly   +3 more sources

Production of 3‐Fucosyllactose in Engineered Escherichia coli with α‐1,3‐Fucosyltransferase from Helicobacter pylori

Biotechnology Journal, 2019
3‐Fucosyllactose (3‐FL), one of the major oligosaccharides in human breast milk, is produced in engineered Escherichia coli. In order to search for a good α‐1,3‐fucosyltransferase, three bacterial α‐1,3‐fucosyltransferases are expressed in engineered E.
Sang-Min, Jung, Yong-Cheol, Park, Jin-Ho, Seo   +2 more
openaire   +2 more sources

Multistrategy Optimization for High-Yield 3-Fucosyllactose Production in Escherichia coli BL21 Star (DE3)

Journal of Agricultural and Food Chemistry
3-Fucosyllactose (3-FL), an essential component of human milk oligosaccharides, is crucial for infant health. However, the extraction of 3-FL from milk presents a significant challenge. In this study, E. coli BL21 Star (DE3) was engineered for 3-FL biosynthesis, and the gene combinations and metabolic pathways were optimized to obtain a high-yield 3-FL
Shujie Lu, Caiwen Lao, Jin Wang, Lixia Yuan, Jianming Yao, He Li, Xijie Fan, Qihong Zhang, Jinyong Wu, Xiangsong Chen   +9 more
openaire   +2 more sources

Diversity-Oriented Enzymatic Synthesis of Human Milk Oligosaccharides Bearing a 3-Fucosyllactose Core

Journal of Agricultural and Food Chemistry
Abstract A notable subset of human milk oligosaccharides (HMOs) bearing an α1,3-fucosylated lactose (3FL) moiety at the reducing end constitutes one of the most abundant and representative classes of HMOs, referred to as 3FL-related HMOs (3FL-HMOs).
Yun Li, Hui Xia, Kan Zhong, Haoxuan Zhong, Huashuai Ding, Xihuan Hu, Zhifei Hu, Hongzhi Cao   +7 more
openaire   +1 more source

Efficient de novo synthesis of 3-fucosyllactose in Escherichia coli via enzyme engineering and pathway refinement

Bioresource Technology
3-Fucosyllactose (3-FL), a functional oligosaccharide in human milk, holds great promise due to its health benefits. However, its microbial production is limited by the low activity of α-1,3-fucosyltransferase and the limited GDP-l-fucose supply. This study combined structure-based modeling and evolutionary analysis to identify five double-site mutants
Ruoyu Jia, Mengli Li, Ming Miao, Tao Zhang   +3 more
openaire   +2 more sources