Results 11 to 20 of about 58,354 (259)

Adenovirus transduction of 3T3-L1 cells [PDF]

Journal of Lipid Research, 2001
3T3-L1 cells offer an excellent model system for studies of differentiation and biochemistry of fat cells. However, these cells are limited in their utility by the low efficiency with which DNA can be introduced by transfection.
David J. Orlicky, Jerome Schaack
doaj   +3 more sources

The 3T3-L1 adipocyte glycogen proteome [PDF]

Proteome Science, 2013
Abstract Background Glycogen is a branched polysaccharide of glucose residues, consisting of α-1-4 glycosidic linkages with α-1-6 branches that together form multi-layered particles ranging in size from 30 nm to 300 nm.
Stapleton, D, Nelson, C, Parsawar, K, Flores-Opazo, M, McClain, D, Parker, G   +5 more
openaire   +3 more sources

Cistanche promotes the adipogenesis of 3T3-L1 preadipocytes

PLOS ONE, 2022
Cistanche deserticola Ma (cistanche) is a traditional herb with a wide range of therapeutic properties. However, no evidence of cistanche’s effect on adipogenesis has been found. The effect of cistanche that promotes the adipogenesis of 3T3-L1 preadipocytes was proved by using MTT spectrophotometry, Nile Red staining, Oil Red O staining and ...
Ping Zhang, Le Su, Xiuyu Ji, Feifan Ma, Qiulin Yue, Chen Zhao, Song Zhang, Xin Sun, Kunlun Li, Lin Zhao   +9 more
openaire   +4 more sources

Development of Insulin Resistance in 3T3-L1 Adipocytes [PDF]

Journal of Biological Chemistry, 1997
Insulin resistance is a manifestation of both diabetes mellitus and obesity. However, the mechanism is still not clearly identified. Herein, we describe a procedure that allows us to evaluate the development of insulin resistance in 3T3-L1 adipocytes. Under these conditions, we show that the concentration of insulin required for 50% desensitization of ...
M J, Thomson, M G, Williams, S C, Frost
openaire   +2 more sources

Inhibition of adipogenesis by Tempol in 3T3-L1 cells [PDF]

Free Radical Biology and Medicine, 2010
Obesity is highly associated with an increased risk of serious health conditions including hypertension, cardiovascular disease, diabetes, and cancer. Changes in redox status with increased oxidative stress have been linked with obesity. Previous studies have shown that administration of the antioxidant Tempol in the food of mice prevents obesity ...
Yuval, Samuni, John A, Cook, Rajani, Choudhuri, William, Degraff, Anastasia L, Sowers, Murali C, Krishna, James B, Mitchell   +6 more
openaire   +2 more sources

AGMO Inhibitor Reduces 3T3-L1 Adipogenesis [PDF]

Cells, 2021
Alkylglycerol monooxygenase (AGMO) is a tetrahydrobiopterin (BH4)-dependent enzyme with major expression in the liver and white adipose tissue that cleaves alkyl ether glycerolipids. The present study describes the disclosure and biological characterization of a candidate compound (Cp6), which inhibits AGMO with an IC50 of 30–100 µM and 5–20-fold ...
Caroline Fischer, Annett Wilken-Schmitz, Victor Hernandez-Olmos, Ewgenij Proschak, Holger Stark, Ingrid Fleming, Andreas Weigert, Manuela Thurn, Martine Hofmann, Ernst R. Werner, Gerd Geisslinger, Ellen Niederberger, Katrin Watschinger, Irmgard Tegeder   +13 more
openaire   +5 more sources

Isorhamnetin Represses Adipogenesis in 3T3‐L1 Cells [PDF]

Obesity, 2009
Adipocyte dysfunction is strongly associated with the development of obesity, which is a major risk factor for many disorders including diabetes, hypertension, and heart disease. It is generally accepted that the regulation of adipogenesis or adipokines expression prevents obesity.
Jongsung, Lee, Eunsun, Jung, Jienny, Lee, Saebom, Kim, Sungran, Huh, Youngsoo, Kim, Yongwoo, Kim, Sang Yo, Byun, Yeong-Shik, Kim, Deokhoon, Park   +9 more
openaire   +2 more sources

Resistin promotes 3T3-L1 preadipocyte differentiation [PDF]

European Journal of Endocrinology, 2004
OBJECTIVE: To investigate the relationship between resistin (a potential link between obesity and type 2 diabetes) and preadipocyte differentiation. DESIGN: A rat resistin expression vector was transfected into 3T3-L1 preadipocytes and differentiation was compared between normal 3T3-L1 cells, rat resistin-transfected cells and non-transfected cells ...
Haixia, Gong, Yuhui, Ni, Xirong, Guo, Li, Fei, Xiaoqin, Pan, Mei, Guo, Ronghua, Chen   +6 more
openaire   +2 more sources

RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease

Hepatology, EarlyView., 2022
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease. Abstract Background and Aims Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD)
Marta B. Afonso, Tawhidul Islam, Julie Magusto, Ricardo Amorim, Véronique Lenoir, Rui F. Simões, José Teixeira, Liana C. Silva, Dominique Wendum, Isabelle Jéru, Corinne Vigouroux, Rui E. Castro, Paulo J. Oliveira, Carina Prip‐Buus, Vlad Ratziu, Jérémie Gautheron, Cecília M. P. Rodrigues   +16 more
wiley   +1 more source

Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system [PDF]

, 2013
Murine 3T3-L1 adipocytes are widely used as a cellular model of obesity. However, whereas transfection of 3T3-L1 preadipocytes is straightforward, ectopic gene expression in mature 3T3-L1 adipocytes has proved challenging.
Achim Leutz, Boizard, Calkhoven, Carlotti, Chapman, Choudhury, Cristina L Esteves, Descombes, Esteves, Gnudi, Jonathan R Seckl, Karen E Chapman, Napolitano, Ossipow, Otto, Park, Ramji, Rosen, Seckl, Shoelson, Simon G Lillico, Smink, Thurmond, Tilghman, Val Kelly, Valérie Bégay, Welm, Wethmar, Yeh   +28 more
core   +2 more sources