Results 21 to 30 of about 58,354 (259)

Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines

Adipocyte, 2021
Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-
Tomás Suchý, Isabell Kaczmarek, Tomislav Maricic, Christian Zieschang, Torsten Schöneberg, Doreen Thor, Ines Liebscher   +6 more
doaj   +1 more source

Sulforaphane induces adipocyte browning and promotes glucose and lipid utilization [PDF]

, 2016
Scope: Obesity is closely related to the imbalance of white adipose tissue storing excess calories, and brown adipose tissue dissipating energy to produce heat in mammals.
Bao, Yong P., Chen, Fen, Chen, Shi Y, Fu, Jian F, Shan, Yu J, Wang, An S, Yao, An J, Zhang, Hui Q, Zhang, Xiao H, Zhao, Jin S, Zhou, Zu Q   +10 more
core   +1 more source

Mouse mesoderm-specific transcript inhibits adipogenic differentiation and induces trans-differentiation into hepatocyte-like cells in 3T3-L1 preadiocytes

BMC Research Notes, 2022
Objective The mesoderm-specific transcript (Mest) is an imprinted gene that is transcribed from the paternal allele. It is a marker of adipose tissue expansion; however, it is uncertain whether Mest expression promotes or suppresses adipogenic ...
Yoshito Kadota, Takashige Kawakami, Masao Sato, Shinya Suzuki   +3 more
doaj   +1 more source

Alternate routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4 [PDF]

, 2015
Insulin-stimulated delivery of glucose transporters (GLUT4) from specialized intracellular GLUT4 storage vesicles (GSVs) to the surface of fat and muscle cells is central to whole-body glucose.
Bryant, Nia J., Gould, Gwyn W., Kioumourtzoglou, Dimitrios, Pryor, Paul R.   +3 more
core   +3 more sources

Homocysteine inhibits adipogenesis in 3T3-L1 preadipocytes [PDF]

Experimental Biology and Medicine, 2011
Hyperhomocysteinemia (HHcy) is a characteristic metabolic abnormality in several pathological conditions, including hypertension, diabetes and alcoholic liver disease. Emerging evidence indicates that adipose tissue contributes to HHcy and homocysteine (Hcy) conversely affects adipose tissue function. However, the specific effect of Hcyon adipogenesis
Zhigang, Wang, Xiaobing, Dou, Tong, Yao, Zhenyuan, Song   +3 more
openaire   +2 more sources

Mouse 3T3-L1 cells acquire resistance against oxidative stress as the adipocytes differentiate via the transcription factor FoxO [PDF]

, 2010
Repression of excessive increase and enlargement of adipocytes that is closely associated with obesity is effective in the prevention and treatment of metabolic syndrome.
Kojima Takayuki, Norose Takanari, Sakamoto Kazuichi, Tsuchiya Keita, 坂本 和一   +4 more
core   +1 more source

Topiramate effects lipolysis in 3T3-L1 adipocytes

Biomedical Reports, 2015
Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3-L1. The 3T3-L1 cells were incubated in 50 µM TPM for 30 min.
Campagnaro Martins, Gabriela Poltronieri, Souza, Camila Oliveira, Marques, Scherolin De Oliveira, Luciano, Thais Fernandes, Da Silva Pieri, Bruno Luiz, Rosa, Jose Cesar, Ramos Da Silva, Adelino Sanchez, Pauli, Jose Rodrigo, Cintra, Dennys Esper, Ropelle, Eduardo Rochete, Rodrigues, Bruno, De Lira, Fabio Santos, De Souza, Claudio Teodoro   +12 more
openaire   +4 more sources

CCR4-NOT2 Promotes the Differentiation and Lipogenesis of 3T3-L1 Adipocytes via Upregulation of PPARγ, CEBPα and Inhibition of P-GSK3α/β and β-Catenin

Cellular Physiology and Biochemistry, 2015
Background/Aims: Though CCR4-NOT2 (CNOT2), one of CCR4-NOT complex subunits, was known to be involved in metastasis and apoptosis through transcription and mRNA degradation, its other biological function is poorly understood so far. The aim of this study
Eun Jung Sohn, Deok-Beom Jung, Jihyun Lee, Sang Wook Yoon, Gun-Ho Won, Hyun Suk Ko, Sung-Hoon Kim   +6 more
doaj   +1 more source

Effect of Astaxanthin on the Inhibition of Lipid Accumulation in 3T3-L1 Adipocytes via Modulation of Lipogenesis and Fatty Acid Transport Pathways

Molecules, 2020
Obesity is defined as a condition of excessive fat tissue accumulation. It was the major factor most closely associated with lifestyle-related diseases.
Mei-Chih Tsai, Shih-Chien Huang, Wei-Tang Chang, Shiuan-Chih Chen, Chin-Lin Hsu   +4 more
doaj   +1 more source

Prolonged induction activates Cebpα independent adipogenesis in NIH/3T3 cells. [PDF]

PLoS ONE, 2013
BACKGROUND: 3T3-L1 cells are widely used to study adipogenesis and insulin response. Their adipogenic potential decreases with time in the culture. Expressing exogenous genes in 3T3-L1 cells can be challenging.
Hsiao-Yun Shao, Hsue-Yin Hsu, Kuan-Sju Wu, Siow-Wey Hee, Lee-Ming Chuang, Jih-I Yeh   +5 more
doaj   +1 more source