Results 11 to 20 of about 43,182 (237)

The 3T3-L1 adipocyte glycogen proteome [PDF]

Proteome Science, 2013
Abstract Background Glycogen is a branched polysaccharide of glucose residues, consisting of α-1-4 glycosidic linkages with α-1-6 branches that together form multi-layered particles ranging in size from 30 nm to 300 nm.
Stapleton, D, Nelson, C, Parsawar, K, Flores-Opazo, M, McClain, D, Parker, G   +5 more
openaire   +3 more sources

Development of Insulin Resistance in 3T3-L1 Adipocytes [PDF]

Journal of Biological Chemistry, 1997
Insulin resistance is a manifestation of both diabetes mellitus and obesity. However, the mechanism is still not clearly identified. Herein, we describe a procedure that allows us to evaluate the development of insulin resistance in 3T3-L1 adipocytes. Under these conditions, we show that the concentration of insulin required for 50% desensitization of ...
M J, Thomson, M G, Williams, S C, Frost
openaire   +2 more sources

RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease

Hepatology, EarlyView., 2022
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease. Abstract Background and Aims Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD)
Marta B. Afonso, Tawhidul Islam, Julie Magusto, Ricardo Amorim, Véronique Lenoir, Rui F. Simões, José Teixeira, Liana C. Silva, Dominique Wendum, Isabelle Jéru, Corinne Vigouroux, Rui E. Castro, Paulo J. Oliveira, Carina Prip‐Buus, Vlad Ratziu, Jérémie Gautheron, Cecília M. P. Rodrigues   +16 more
wiley   +1 more source

MicroRNA and 3T3-L1 pre-adipocyte differentiation [PDF]

RNA, 2006
MicroRNAs (miRNAs) have been suggested to play important roles in cell proliferation, apoptosis, and differentiation. In this study, we examined miRNA expression profiles during 3T3-L1 pre-adipocyte differentiation. We constructed miRNA libraries from pre- and post-differentiated 3T3-L1 cells, and identified the expression of 77 previously reported ...
Kazuaki, Kajimoto, Hiroaki, Naraba, Naoharu, Iwai   +2 more
openaire   +2 more sources

Phosphatidylcholine induces apoptosis of 3T3-L1 adipocytes [PDF]

Journal of Biomedical Science, 2011
Abstract Background Phosphatidylcholine (PPC) formulation is used for lipolytic injection, even though its mechanism of action is not well understood. Methods The viability of 3T3-L1 pre-adipocytes and differentiated 3T3-L1 cells was measured after treatment of PPC alone ...
Li Hailan, Lee Jong-Hyuk, Kim Su Yeon, Yun Hye-Young, Baek Kwang Jin, Kwon Nyoun Soo, Yoon Yoosik, Jeong Ji Hoon, Kim Dong-Seok   +8 more
openaire   +3 more sources

Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. [PDF]

PLoS ONE, 2014
Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human ...
Kana Inoue, Norikazu Maeda, Takuya Mori, Ryohei Sekimoto, Yu Tsushima, Keisuke Matsuda, Masaya Yamaoka, Takayoshi Suganami, Hitoshi Nishizawa, Yoshihiro Ogawa, Tohru Funahashi, Iichiro Shimomura   +11 more
doaj   +1 more source

Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation [PDF]

, 2020
The chronic low-grade inflammation in adipose tissue plays a causal role in obesity-induced insulin resistance and its associated pathophysiological consequences. In this study, we investigated the effects of extracts of Broussonetia papyrifera root bark
Choi, Jang Hyun, Choi, Sun Sil, Jang, Hyunduk, Khim, Keon Woo, Lee, Jae Min, Lee, Yo Han, Oh, Sei Ryang, Park, Jiyoung, Park, Mi Hyeon, Ryu, Hyung Won   +9 more
core   +1 more source

Spermidine is indispensable in differentiation of 3T3‐L1 fibroblasts to adipocytes [PDF]

Journal of Cellular and Molecular Medicine, 2010
AbstractImpaired adipogenesis has been shown to predispose to disturbed adipocyte function and development of metabolic abnormalities. Previous studies indicate that polyamines are essential in the adipogenesis in 3T3‐L1 fibroblasts. However, the specific roles of individual polyamines during adipogenesis have remained ambiguous as the natural ...
Vuohelainen, Susanna, Pirinen, Eija, Cerrada-Gimenez, Marc, Keinänen, Tuomo A., Uimari, Anne, Pietilä, Marko, Khomutov, Alex R., Jänne, Juhani, Alhonen, Leena   +8 more
openaire   +2 more sources

Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes. [PDF]

Antioxidants (Basel), 2023
Crocetin (CCT) is a natural saffron-derived apocarotenoid that possesses healthy properties such as anti-adipogenic, anti-inflammatory, and antioxidant activities. Lipolysis is enhanced in obesity and correlates with a pro-inflammatory, pro-oxidant state. In this context, we aimed to investigate whether CCT affects lipolysis. To evaluate CCT’s possible
Cimas FJ, De la Cruz-Morcillo MÁ, Cifuentes C, Moratalla-López N, Alonso GL, Nava E, Llorens S.   +6 more
europepmc   +5 more sources

Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system [PDF]

, 2013
Murine 3T3-L1 adipocytes are widely used as a cellular model of obesity. However, whereas transfection of 3T3-L1 preadipocytes is straightforward, ectopic gene expression in mature 3T3-L1 adipocytes has proved challenging.
Achim Leutz, Boizard, Calkhoven, Carlotti, Chapman, Choudhury, Cristina L Esteves, Descombes, Esteves, Gnudi, Jonathan R Seckl, Karen E Chapman, Napolitano, Ossipow, Otto, Park, Ramji, Rosen, Seckl, Shoelson, Simon G Lillico, Smink, Thurmond, Tilghman, Val Kelly, Valérie Bégay, Welm, Wethmar, Yeh   +28 more
core   +2 more sources