Results 11 to 20 of about 4,897,037 (304)

Adenovirus transduction of 3T3-L1 cells [PDF]

open access: yesJournal of Lipid Research, 2001
3T3-L1 cells offer an excellent model system for studies of differentiation and biochemistry of fat cells. However, these cells are limited in their utility by the low efficiency with which DNA can be introduced by transfection.
David J. Orlicky, Jerome Schaack
doaj   +3 more sources

The orientation of centrioles in migrating 3T3 cells

open access: yesExperimental Cell Research, 1979
Abstract Based on electron microscopic examination of 20 migrating 3T3 cells, the paper suggests that one centriole of a pair is preferentially oriented perpendicular, the other parallel to the substrate. The finding is interpreted as further support for the possibility that centrioles are involved in the control of migration in 3T3 cells.
G, Albrecht-Buehler, A, Bushnell
openaire   +3 more sources

The 89,000-Mr murine cytomegalovirus immediate-early protein stimulates c-fos expression and cellular DNA synthesis [PDF]

open access: yes, 1988
The immediate-early (IE) genes of murine cytomegalovirus (MCMV) are expressed in the absence of prior viral protein synthesis and regulate the transcription of MCMV early genes. The effect of MCMV IE genes on growth induction was studied.
Philipson, L.   +11 more
core   +2 more sources

MiR-146a-5p, targeting ErbB4, promotes 3T3-L1 preadipocyte differentiation through the ERK1/2/PPAR-γ signaling pathway

open access: yesLipids in Health and Disease, 2022
Background MicroRNAs (MiRNAs) are known to participate in preadipocyte differentiation, but the manner in which miR-146a-5p participates in this process remains unclear.
Yifen Wang   +5 more
doaj   +1 more source

Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of Obesity

open access: yesLife, 2023
The increasing prevalence of obesity has prompted intensive research into understanding its role in pathogenesis and designing appropriate treatments. To determine the signals generated from the interaction of fat cells with a target organ, a reliable ...
Noshin Mubtasim, Lauren Gollahon
doaj   +1 more source

Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells

open access: yesJournal of Lipid Research, 2001
3T3-L1 cells have been used as a model to study the differentiation and physiology of adipocytes. Exogenous expression of proteins in these cells offers the prospect of understanding the protein's function(s) in adipose tissue.
David J. Orlicky   +2 more
doaj   +1 more source

A novel 3T3-L1 preadipocyte variant that expresses PPARγ2 and RXRα but does not undergo differentiation

open access: yesJournal of Lipid Research, 1998
This report describes a novel adipocyte-like cell line termed 3T3-L1/RB1 that was derived from preadipocyte cell line, 3T3-L1. The 3T3-L1/RB1 cells continued to divide after reaching confluence, formed foci, and constitutively expressed a low level of ...
Rebecca A. Baillie   +3 more
doaj   +1 more source

Cross-talk between human airway epithelial cells and 3T3-J2 feeder cells involves partial activation of human MET by murine HGF. [PDF]

open access: yesPLoS ONE, 2018
There is considerable interest in the ex vivo propagation of primary human basal epithelial stem/progenitor cells with a view to their use in drug development, toxicity testing and regenerative medicine.
Robert E Hynds   +7 more
doaj   +1 more source

Metformin Improves Burn Wound Healing by Modulating Microenvironmental Fibroblasts and Macrophages

open access: yesCells, 2022
Metformin, a biguanide, exerts different functions through various signaling pathways. In order to investigate the function and mechanism of metformin in burn wounds, we established burn rat models, subcutaneously injected metformin to treat the wounds ...
Liangliang Shi   +9 more
doaj   +1 more source

Mouse mesoderm-specific transcript inhibits adipogenic differentiation and induces trans-differentiation into hepatocyte-like cells in 3T3-L1 preadiocytes

open access: yesBMC Research Notes, 2022
Objective The mesoderm-specific transcript (Mest) is an imprinted gene that is transcribed from the paternal allele. It is a marker of adipose tissue expansion; however, it is uncertain whether Mest expression promotes or suppresses adipogenic ...
Yoshito Kadota   +3 more
doaj   +1 more source

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