Results 21 to 30 of about 2,171 (168)

The 5-Hydroxymethylcytosine (5hmC) Reader UHRF2 Is Required for Normal Levels of 5hmC in Mouse Adult Brain and Spatial Learning and Memory [PDF]

Journal of Biological Chemistry, 2017
UHRF2 has been implicated as a novel regulator for both DNA methylation (5mC) and hydroxymethylation (5hmC), but its physiological function and role in DNA methylation/hydroxymethylation are unknown. Here we show that in mice, UHRF2 is more abundantly expressed in the brain and a few other tissues.
Ruoyu, Chen, Qiao, Zhang, Xiaoya, Duan, Philippe, York, Guo-Dong, Chen, Pengcheng, Yin, Haijun, Zhu, Meichen, Xu, Peilin, Chen, Qihan, Wu, Dali, Li, Jacques, Samarut, Guoliang, Xu, Pumin, Zhang, Xiaohua, Cao, Jiwen, Li, Jiemin, Wong   +16 more
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Correlated 5-Hydroxymethylcytosine (5hmC) and Gene Expression Profiles Underpin Gene and Organ-Specific Epigenetic Regulation in Adult Mouse Brain and Liver. [PDF]

PLoS ONE, 2017
DNA methylation is an epigenetic mechanism essential for gene regulation and vital for mammalian development. 5-hydroxymethylcytosine (5hmC) is the first oxidative product of the TET-mediated 5-methylcytosine (5mC) demethylation pathway. Aside from being
I-Hsuan Lin, Yi-Fan Chen, Ming-Ta Hsu
doaj   +1 more source

-OH No! hiPSCs Misplace Their 5hmCs [PDF]

Cell Stem Cell, 2013
hiPSCs and hESCs are thought to display subtle genetic and epigenetic variability. Recently in Nature Cell Biology, Wang et al. (2013) demonstrated a role for TET1 during reprogramming of human cells and showed that hiPSCs lack appropriate 5hmC marks in subtelomeric regions, contributing to epigenetic variation common to hiPSCs.
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TET Enzymes and 5hmC in Adaptive and Innate Immune Systems [PDF]

Frontiers in Immunology, 2019
DNA methylation is an abundant and stable epigenetic modification that allows inheritance of information from parental to daughter cells. At active genomic regions, DNA methylation can be reversed by TET (Ten-eleven translocation) enzymes, which are responsible for fine-tuning methylation patterns.
Chan-Wang J. Lio, Anjana Rao, Anjana Rao, Anjana Rao   +3 more
openaire   +3 more sources

5-hydroxymethyl Cytosine (5hmC) enrichment

, 2021
Abstract Here we present a method for 5-hydroxymethyl cytosine enrichment from cell free DNA or fragmented genomic DNA. This method is based on Song et al (1).
Chris Ellison, Tierney Phillips, Erin McCarthy, Gulfem Guler, Samuel Levy   +4 more
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Global DNA 5hmC and CK19 with 5hmC positives cell contents represent a promising biomarker for predicting prognosis in small hepatocellular carcinoma [PDF]

, 2020
Abstract Background: 5-Hydroxymethylcytosine (5hmC) exists dynamically and exhibits various regulatory functions. It’s possibly associated with tumor occurrence and malignant transformation. Nevertheless, the part of 5hmC in small hepatocellular carcinoma (SHCC) is still elusive. The study was directed toward characterizing 5hmC content in SHCC
Jinhua Jiang, Tinghua Yan, Dan Cui, Jun Wang, Fang Guo, Jialin Shen   +5 more
openaire   +1 more source

Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA

Epigenetics & Chromatin, 2017
Background The discovery that 5-methylcytosine (5mC) can be oxidized to 5-hydroxymethylcytosine (5hmC) by the ten-eleven translocation (TET) proteins has prompted wide interest in the potential role of 5hmC in reshaping the mammalian DNA methylation ...
Ksenia Skvortsova, Elena Zotenko, Phuc-Loi Luu, Cathryn M. Gould, Shalima S. Nair, Susan J. Clark, Clare Stirzaker   +6 more
doaj   +1 more source

Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue

Epigenetics, 2020
Despite recent evidence that 5-hydroxymethylcytosine (5hmC) possesses roles in gene regulation distinct from 5-methylcytosine (5mC), relatively little is known regarding the functions of 5hmC in mammalian tissues.
Owen M. Wilkins, Kevin C. Johnson, E. Andres Houseman, Jessica E. King, Carmen J. Marsit, Brock C. Christensen   +5 more
doaj   +1 more source

5hmC modification regulates R-loop accumulation in response to stress

Frontiers in Psychiatry, 2023
R-loop, an RNA-DNA hybrid structure, arises as a transcriptional by-product and has been implicated in DNA damage and genomic instability when excessive R-loop is accumulated. Although previous study demonstrated that R-loop is associated with ten-eleven translocation (Tet) proteins, which oxidize 5-methylcytosine to 5-hydroxymethylcytosine (5hmC), the
Xingyun Xu, Xingyun Xu, Junjie Wang, Wenjuan Wang, Yutong Zhang, Bo Wan, Zhigang Miao, Xingshun Xu, Xingshun Xu, Xingshun Xu   +9 more
openaire   +3 more sources

Recognition of 5-hydroxymethylcytosine by the Uhrf1 SRA domain. [PDF]

, 2011
Recent discovery of 5-hydroxymethylcytosine (5hmC) in genomic DNA raises the question how this sixth base is recognized by cellular proteins. In contrast to the methyl-CpG binding domain (MBD) of MeCP2, we found that the SRA domain of Uhrf1, an essential
Frauer, C., M. Cristina Cardoso, Leonhardt, Heinrich, Frauer, Carina, Bultmann, Sebastian, Casa, V., Valentina Casa, Frauer Carina, Hoffmann, T., Cardoso, M. Cristina, Leonhardt Heinrich, Antes, I., Casa Valentina, Iris Antes, Leonhart, H., Heinrich Leonhardt, Cardoso M. Cristina, Hoffmann Thomas, Hoffmann, Thomas, Antes Iris, Thomas Hoffmann, M Cristina Cardoso, Bultmann Sebastian, Bultmann, S., Antes, Iris, Casa, Valentina, Carina Frauer, Sebastian Bultmann   +27 more
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