Results 1 to 10 of about 733,018 (171)

From affinity selection to kinetic selection in Germinal Centre modelling. [PDF]

PLoS Computational Biology, 2022
Affinity maturation is an evolutionary process by which the affinity of antibodies (Abs) against specific antigens (Ags) increases through rounds of B-cell proliferation, somatic hypermutation, and positive selection in germinal centres (GC).
Danial Lashgari, Elena Merino Tejero, Michael Meyer-Hermann, Mathieu A F Claireaux, Marit J van Gils, Huub C J Hoefsloot, Antoine H C van Kampen   +6 more
doaj   +6 more sources

Enantioselective protein affinity selection mass spectrometry (E-ASMS) [PDF]

Nature Communications
We report an enantioselective protein affinity selection mass spectrometry screening approach (E-ASMS) that enables the detection of weak binders, informs on selectivity, and generates orthogonal confirmation of binding.
Xiaoyun Wang, Jianxian Sun, Shabbir Ahmad, Diwen Yang, Fengling Li, U. Hang Chan, Hong Zeng, Conrad V. Simoben, Stuart R. Green, Madhushika Silva, Scott Houliston, Aiping Dong, Albina Bolotokova, Elisa Gibson, Maria Kutera, Pegah Ghiabi, Ivan Kondratov, Tetiana Matviyuk, Alexander Chuprina, Danai Mavridi, Christopher Lenz, Andreas C. Joerger, Benjamin D. Brown, Richard B. Heath, Wyatt W. Yue, Lucy K. Robbie, Tyler S. Beyett, Susanne Müller, Stefan Knapp, Dafydd R. Owen, Rachel Harding, Matthieu Schapira, Peter J. Brown, Vijayaratnam Santhakumar, Suzanne Ackloo, Cheryl H. Arrowsmith, Aled M. Edwards, Hui Peng, Levon Halabelian   +38 more
doaj   +4 more sources

Affinity Selection in Germinal Centers: Cautionary Tales and New Opportunities [PDF]

Cells, 2021
Our current quantitative knowledge of the kinetics of antibody-mediated immunity is partly based on idealized experiments throughout the last decades.
Jose Faro, Mario Castro
doaj   +2 more sources

Selection of imprinted nanoparticles by affinity chromatography [PDF]

Biosensors and Bioelectronics, 2009
Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography. Subsequent fractionation of these particles by affinity chromatography allowed the separation of high affinity fractions from the mixture of nanoparticles.
Guerreiro, Antonio R., Chianella, Iva, Piletska, Elena V., Whitcombe, Michael J., Piletsky, Sergey A.   +4 more
openaire   +6 more sources

Advances in high‐throughput mass spectrometry in drug discovery

EMBO Molecular Medicine, 2022
High‐throughput (HT) screening drug discovery, during which thousands or millions of compounds are screened, remains the key methodology for identifying active chemical matter in early drug discovery pipelines.
Maria Emilia Dueñas, Rachel E Peltier‐Heap, Melanie Leveridge, Roland S Annan, Frank H Büttner, Matthias Trost   +5 more
doaj   +1 more source

Expansion and Refinement of Deep Sequence-Coupled Biopanning Technology for Epitope-Specific Antibody Responses in Human Serum

Viruses, 2020
Identifying the specific epitopes targeted by antibodies elicited in response to infectious diseases is important for developing vaccines and diagnostics.
Nikole L. Warner, Alexandria C. Linville, Susan B. Core, Brechla Moreno, Juan M. Pascale, David S. Peabody, Bryce Chackerian, Kathryn M. Frietze   +7 more
doaj   +1 more source

Positive Selection in the Light Zone of Germinal Centers

Frontiers in Immunology, 2021
Germinal centers (GCs) are essential sites for the production of high-affinity antibody secreting plasma cells (PCs) and memory-B cells (MBCs), which form the framework of vaccination.
Rinako Nakagawa, Dinis Pedro Calado, Dinis Pedro Calado   +2 more
doaj   +1 more source

Antibodies to Variable Domain 4 Linear Epitopes of the Chlamydia trachomatis Major Outer Membrane Protein Are Not Associated with Chlamydia Resolution or Reinfection in Women

mSphere, 2020
Chlamydia trachomatis is an obligate intracellular bacterium. C. trachomatis infection is the most prevalent bacterial sexually transmitted infection and can lead to pelvic inflammatory disease and infertility in women. There is no licensed vaccine for C.
Amanda L. Collar, Alexandria C. Linville, Susan B. Core, Cosette M. Wheeler, William M. Geisler, David S. Peabody, Bryce Chackerian, Kathryn M. Frietze   +7 more
doaj   +1 more source

Affinity Maturation of Monoclonal Antibody 1E11 by Targeted Randomization in CDR3 Regions Optimizes Therapeutic Antibody Targeting of HER2-Positive Gastric Cancer. [PDF]

PLoS ONE, 2015
Anti-HER2 murine monoclonal antibody 1E11 has strong and synergistic anti-tumor activity in HER2-overexpressing gastric cancer cells when used in combination with trastuzumab. We presently optimized this antibody for human therapeutics.
Bong-Kook Ko, Soyoung Choi, Lei Guang Cui, Young-Ha Lee, In-Sik Hwang, Kyu-Tae Kim, Hyunbo Shim, Jong-Seo Lee   +7 more
doaj   +1 more source

Robust Unsupervised Hyperspectral Band Selection via Global Affinity Matrix Reconstruction

IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, 2023
Unsupervised band selection is fundamental to alleviate the curse of dimensionality for hyperspectral imagery. Although many research works have been developed, it is still a challenging problem to improve the poor classification performance with a small
Mengbo You, Aihong Yuan, Min Zou, Kouichi Konno   +3 more
doaj   +1 more source