Results 201 to 210 of about 27,782 (234)

Complete sequencing and characterization of equine aggrecan

Veterinary and Comparative Orthopaedics and Traumatology, 2015
SummaryObjectives: To fully sequence and characterize equine aggrecan and confirm conservation of major aggrecanase, calpain and matrix metalloproteinase (MMP) cleavage sites.Methods: Reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends were used to generate clones that encompassed the complete equine aggrecan sequence ...
E H G, Caporali, T, Kuykendall, M C, Stewart   +2 more
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Brain aggrecan.

Perspectives on developmental neurobiology, 1997
During development, the extracellular matrix (ECM) is a complex dynamic structure whose components and organization help to establish the requisite position and state of differentiation. Until recently, the large chondroitin sulfate proteoglycan, aggrecan, has been localized predominantly to skeletal tissue and considered a hallmark of cartilage ...
N B, Schwartz, M, Domowicz, R C, Krueger, H, Li, D, Mangoura   +4 more
openaire   +1 more source

Aggrecan, aging and assembly in articular cartilage

Cellular and Molecular Life Sciences, 2005
The primary function of articular cartilage to act as a self-renewing, low frictional material that can distribute load efficiently at joints is critically dependent upon the composition and organisation of the extracellular matrix. Aggrecan is a major component of the extracellular matrix, forming high molecular weight aggregates necessary for the ...
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Hyaluronics and aggrecanics

2002
Hyaluronan (HA) is a high molecular weight (105-107Da) unbranched glycosaminoglycan, composed of repeating disaccharides of D-N-Acetylglucosamine and D-Glucuronic acid. It is a widely distributed component of the extracellular matrix of vertebrate tissues [1].
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Changes in aggrecan populations in experimental osteoarthritis

Osteoarthritis and Cartilage, 1994
Adult articular cartilage contains at least two distinct populations of aggrecan: one is larger and richer in chondroitin sulfate (CS), while the other is smaller with less CS. The smaller form is thought to be derived from the larger. The amount of CS in cartilage decreases with maturation and aging, mainly because of a decrease in the proportion of ...
openaire   +2 more sources

Sensitivity of monoclonal antibody, 5-D-4, for the detection of aggrecan, aggrecan fragments, and keratan sulfate

Agents and Actions, 1993
Bovine nasal septum aggrecan and selected proteinase-digested products of aggrecan were evaluated in an inhibition ELISA using the anti-keratan sulfate (KS) monoclonal antibody 5-D-4 (5D4). Undegraded aggrecan was recognized with an IC50 of 0.27 microgram/ml.
C A, Saphos, P, Dey, M W, Lark, V L, Moore   +3 more
openaire   +2 more sources

Quantitative analysis of the effects of hyaluronan and aggrecan concentration and hyaluronan size on the elasticity of hyaluronan–aggrecan solutions

Biorheology: The Official Journal of the International Society of Biorheology, 2004
We determined elasticity (G′) and viscosity (G″) of various aggrecan–hyaluronan solutions using a controlled‐stress rheometer with high (10 Hz) to low (0.1 Hz) frequencies. Aggrecan solution (50 mg/ml) alone showed little elasticity at any frequency, but the addition of 3300 kDa hyaluronan at 0.001–0.1 mg/ml markedly increased the elasticity, but not ...
M, Nishimura, M, Kawata, W, Yan, A, Okamato, H, Nishimura, Y, Ozaki, T, Hamada, Y, Kato   +7 more
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Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry

2011
Aggrecan is essential for the normal function of articular cartilage and intervertebral disc, where it provides the ability for the tissues to withstand compressive loading. This property depends on both the high charge density endowed by its numerous chondroitin sulfate and keratan sulfate chains and its ability to form large molecular aggregates via ...
Peter J, Roughley, John S, Mort
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Aggrecan: Approaches to Study Biophysical and Biomechanical Properties

2014
Aggrecan, the most abundant extracellular proteoglycan in cartilage (~35 % by dry weight), plays a key role in the biophysical and biomechanical properties of cartilage. Here, we review several approaches based on atomic force microscopy (AFM) to probe the physical, mechanical, and structural properties of aggrecan at the molecular level.
Hadi Tavakoli, Nia, Christine, Ortiz, Alan, Grodzinsky   +2 more
openaire   +3 more sources

Aggrecan

2011
openaire   +2 more sources