Results 171 to 180 of about 12,502 (214)
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Detection of lectin activity in Leishmania promastigotes and amastigotes
Acta Tropica, 1997Cell lysates from 16 strains of eight Leishmania species were used to test haemagglutination activity (HA) against a variety of RBC. HA was detected using native or neuraminidase-treated rabbit RBC; it was found in promastigotes of all the Leishmania strains tested and in axenic amastigotes of L. mexicana.
Svobodová, M, Bates, P A, Volf, P
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In vivo Assay of Viability of Amastigotes of Leishmania donovani
The Journal of Parasitology, 1980Following intravenous (i.v.) injection of formalin-killed or ultraviolet-killed or ultraviolet-irradiated amastigotes of Leishmania donovani, the parasites could be identified in mouse liver at 5 min, but most amastigotes were digested by 3 hr or between 24 and 72 hr, respectively.
V H, Hodgkinson, R, Herman
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Characterization of Leishmania (Leishmania) tropica axenic amastigotes
Acta Tropica, 2010Optimum conditions for generating Leishmania (Leishmania) tropica axenic amastigotes (AxA) in culture were determined, pH 5.5/36 degrees C, and the parasites characterized by different techniques, including light microscopy, macrophage infection, stage specific antigen expression and differential display. AxA were morphologically similar to amastigotes
Abedelmajeed, Nasereddin +3 more
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Electrophoretic analysis of the polypeptides of Leishmania amastigotes and promastigotes
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1986The polypeptides of Leishmania mexicana mexicana (M379), L. m. amazonensis (LV78), L. major (LV39) and L. d. donovani (LV39) amastigotes and cultured promastigotes have been analysed by SDS-polyacrylamide gel electrophoresis. The polypeptide banding patterns of the promastigotes of the four species were quite similar, but distinct differences were ...
M F, Pupkis, G H, Coombs
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In vitro cultivation and characterization of axenic amastigotes of Leishmania
Trends in Parasitology, 2001The establishment of axenic cultures of the amastigote stage of Leishmania is important to understand the mechanisms regulating the differentiation, survival and pathogenicity of the parasite with a view to develop and identify molecular and chemotherapeutic targets.
N, Gupta, N, Goyal, A K, Rastogi
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Leishmania mexicana: Amastigote hydrolases in unusual lysosomes
Experimental Parasitology, 1986Leishmania mexicana mexicana (M379) amastigotes were found to contain much higher activities than cultured promastigotes of five putative lysosomal enzymes: cysteine proteinase; arylsulfatase (EC 3.1.6.1); beta-glucuronidase (EC 3.2.1.31); DNase (EC 3.1.22.1), and RNase (EC 3.1.27.1).
L Tetley +2 more
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Macromolecular Synthesis in Leishmania donovani Amastigotes
The Journal of Parasitology, 1985Amastigotes (non-flagellated tissue forms) of Leishmania sp. reside and multiply within the phagolysosomes in tissue macrophages of vertebrate hosts. They are true obligate, intracellular parasites and do not persist as viable entities outside of host cells.
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Purine metabolism in Leishmania donovani amastigotes and promastigotes
Molecular and Biochemical Parasitology, 1983Purine metabolism in Leishmania donovani amastigotes was found to be similar to that of promastigotes with the exception of adenosine metabolism. Adenosine kinase activity in amastigotes is approximately 50-fold greater than in promastigotes. Amastigotes deaminate adenosine to inosine through adenosine deaminase, an enzyme not present in promastigotes.
D L, Looker, R L, Berens, J J, Marr
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Killing of Leishmania Donovani Amastigotes by Murine Macrophages
1982Activated tissue macrophages are the effector cells responsible for containment of a variety of intracellular, bacterial, mycotic and protozoan parasites. The term “activated” macrophage employed originally to describe enhanced bactericidal capability (1) has been extended in recent years to tumoricidal activity (2,3).
P F, Bonventre, C G, Haidaris
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Sterol composition and biosynthesis in Trypanosoma cruzi amastigotes
Molecular and Biochemical Parasitology, 1999A detailed analysis of the endogenous sterols present in the clinically relevant intracellular (amastigote) stages of Trypanosoma cruzi, is presented. The parasites were grown in cultured Vero cells in the absence or presence of different sterol biosynthesis inhibitors, including the C14alpha demethylase inhibitor ketoconazole and two inhibitors of ...
A, Liendo +4 more
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