Results 31 to 40 of about 238,366 (195)

Core of the saliva microbiome: an analysis of the MG-RAST data

BMC Oral Health, 2021
Background Oral microbiota is considered as the second most complex in the human body and its dysbiosis can be responsible for oral diseases. Interactions between the microorganism communities and the host allow establishing the microbiological proles ...
Simone G. Oliveira, Rafaela R. Nishiyama, Claudio A. C. Trigo, Ana Luiza Mattos-Guaraldi, Alberto M. R. Dávila, Rodrigo Jardim, Flavio H. B. Aguiar   +6 more
doaj   +1 more source

VarDict: a novel and versatile variant caller for next-generation sequencing in cancer research [PDF]

, 2016
Accurate variant calling in next generation sequencing (NGS) is critical to understand cancer genomes better. Here we present VarDict, a novel and versatile variant caller for both DNA- and RNA-sequencing data.
Ahdesmaki, Miika, Barrett, J. Carl, Chapman, Brad, Dougherty, Brian, Dry, Jonathan R., Hofmann, Oliver, Johnson, Justin, Lai, Zhongwu, Markovets, Aleksandra, McEwen, Robert   +9 more
core   +2 more sources

Correction: Amplicon–Based Metagenomic Analysis of Mixed Fungal Samples Using Proton Release Amplicon Sequencing

PLoS ONE, 2014
doaj   +5 more sources

Illumina sequencing of 15 deafness genes using fragmented amplicons [PDF]

, 2014
BACKGROUND: Resequencing of deafness related genes using GS FLX massive parallel sequencing of PCR amplicons spanning selected genes has previously been reported as a successful strategy to discover causal variants.
Coucke, Paul, De Keulenaer, Sarah, De Schrijver, Joachim, Deforce, Dieter, Van Criekinge, Wim, Van Nieuwerburgh, Filip, Vandesompele, Jo   +6 more
core   +1 more source

Reconciliation between operational taxonomic units and species boundaries [PDF]

, 2017
The development of high-throughput sequencing technologies has revolutionised the field of microbial ecology via 16S rRNA gene amplicon sequencing approaches.
Boon, Nico, Kerckhof, Frederiek-Maarten, Leys, Natalie, Monsieurs, Pieter, Mysara, Mohamed, Props, Ruben, Raes, Jeroen, Vandamme, Peter   +7 more
core   +2 more sources

Study of microbiocenosis of canine dental biofilms

Scientific Reports, 2021
Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood.
Jana Kačírová, Aladár Maďari, Rastislav Mucha, Lívia K. Fecskeová, Izabela Mujakic, Michal Koblížek, Radomíra Nemcová, Marián Maďar   +7 more
doaj   +1 more source

An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing

Frontiers in Microbiology, 2021
In microbiome research, phylogenetic and functional marker gene amplicon sequencing is the most commonly-used community profiling approach. Consequently, a plethora of protocols for the preparation and multiplexing of samples for amplicon sequencing have
Petra Pjevac, Petra Pjevac, Bela Hausmann, Bela Hausmann, Jasmin Schwarz, Jasmin Schwarz, Gudrun Kohl, Gudrun Kohl, Craig W. Herbold, Alexander Loy, Alexander Loy, David Berry, David Berry   +12 more
doaj   +1 more source

High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa). [PDF]

, 2018
CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high mutation efficiencies have been reported in primary transformants. However, many of the mutations analyzed were somatic and therefore not heritable.
Bertier, Lien D, Bradford, Kent J, Britt, Anne B, Huo, Heqiang, Michelmore, Richard W, Ron, Mily   +5 more
core   +2 more sources

Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections

BMC Genomics, 2017
Background Amplicon deep sequencing permits sensitive detection of minority clones and improves discriminatory power for genotyping multi-clone Plasmodium falciparum infections.
Anita Lerch, Cristian Koepfli, Natalie E. Hofmann, Camilla Messerli, Stephen Wilcox, Johanna H. Kattenberg, Inoni Betuela, Liam O’Connor, Ivo Mueller, Ingrid Felger   +9 more
doaj   +1 more source

MT-Toolbox: improved amplicon sequencing using molecule tags [PDF]

BMC Bioinformatics, 2014
Abstract Background Short oligonucleotides can be used as markers to tag and track DNA sequences. For example, barcoding techniques (i.e. Multiplex Identifiers or Indexing) use short oligonucleotides to distinguish between reads from different DNA samples pooled for high-throughput sequencing.
Yourstone, Scott M, Lundberg, Derek S, Dangl, Jeffery L, Jones, Corbin D   +3 more
openaire   +2 more sources