Results 11 to 20 of about 394,452 (270)

Antibody Multispecificity Mediated by Conformational Diversity

Science, 2003
A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre–steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing ...
James L. C., Roversi P., Tawfik D. S.
openaire   +6 more sources

Beyond Allotypes: The Influence of Allelic Diversity in Antibody Constant Domains

Frontiers in Immunology, 2020
Polymorphic diversity in antibody constant domains has long been defined by allotypic motifs that cross react with the sera of other individuals. Improvements in sequencing technologies have led to the discovery of a large number of new allelic sequences
Annmaree K. Warrender, William Kelton
doaj   +1 more source

Common light chain chickens produce human antibodies of high affinity and broad epitope coverage for the engineering of bispecifics

mAbs, 2021
Bispecific antibodies are an important and growing segment in antibody therapeutics, particularly in the immuno-oncology space. Manufacturing of a bispecific antibody with two different heavy chains is greatly simplified if the light chains can be the ...
Kathryn H. Ching, Kimberley Berg, Kevin Reynolds, Darlene Pedersen, Alba Macias, Yasmina N. Abdiche, William D. Harriman, Philip A. Leighton   +7 more
doaj   +1 more source

Quantitative and Qualitative Analysis of Humoral Immunity Reveals Continued and Personalized Evolution in Chronic Viral Infection

Cell Reports, 2020
Summary: Control of established chronic lymphocytic choriomeningitis virus (LCMV) infection requires the production of neutralizing antibodies, but it remains unknown how the ensemble of antibodies evolves during ongoing infection.
Nike Julia Kräutler, Alexander Yermanos, Alessandro Pedrioli, Suzanne P.M. Welten, Dominique Lorgé, Ute Greczmiel, Ilka Bartsch, Jörg Scheuermann, Jonathan D. Kiefer, Klaus Eyer, Ulrike Menzel, Victor Greiff, Dario Neri, Tanja Stadler, Sai T. Reddy, Annette Oxenius   +15 more
doaj   +1 more source

Assessment of antibody library diversity through next generation sequencing and technical error compensation. [PDF]

PLoS ONE, 2017
Antibody libraries are important resources to derive antibodies to be used for a wide range of applications, from structural and functional studies to intracellular protein interference studies to developing new diagnostics and therapeutics. Whatever the
Marco Fantini, Luca Pandolfini, Simonetta Lisi, Michele Chirichella, Ivan Arisi, Marco Terrigno, Martina Goracci, Federico Cremisi, Antonino Cattaneo   +8 more
doaj   +1 more source

A window into the human immune system: comprehensive characterization of the complexity of antibody complementary-determining regions in functional antibodies

mAbs, 2023
The human immune system uses antibodies to neutralize foreign antigens. They are composed of heavy and light chains, both with constant and variable regions. The variable region has six hypervariable loops, also known as complementary-determining regions
Oscar Mejias-Gomez, Andreas V. Madsen, Kerstin Skovgaard, Lasse E. Pedersen, J. Preben Morth, Timothy P. Jenkins, Peter Kristensen, Steffen Goletz   +7 more
doaj   +1 more source

A Novel Human scFv Library with Non-Combinatorial Synthetic CDR Diversity. [PDF]

PLoS ONE, 2015
The present work describes the construction and validation of a human scFv library with a novel design approach to synthetic complementarity determining region (CDR) diversification.
Xuelian Bai, Jihye Kim, Seungmin Kang, Wankyu Kim, Hyunbo Shim   +4 more
doaj   +1 more source

The antibody loci of the domestic goat (Capra hircus) [PDF]

, 2018
The domestic goat (Capra hircus) is an important ruminant species both as a source of antibody-based reagents for research and biomedical applications and as an economically important animal for agriculture, particularly for developing nations that ...
Bickhart, Derek M., Hammond, John A., Philp, Rebecca L., Schwartz, John C., Smith, Timothy P.L.   +4 more
core   +1 more source

Epigenomic Modifications Mediating Antibody Maturation

Frontiers in Immunology, 2018
Epigenetic modifications, such as histone modifications, DNA methylation status, and non-coding RNAs (ncRNA), all contribute to antibody maturation during somatic hypermutation (SHM) and class-switch recombination (CSR).
Emily C. Sheppard, Rikke Brandstrup Morrish, Michael J. Dillon, Rebecca Leyland, Richard Chahwan   +4 more
doaj   +1 more source

A novel approach to probe host-pathogen interactions of bovine digital dermatitis, a model of a complex polymicrobial infection [PDF]

, 2016
Background: Polymicrobial infections represent a great challenge for the clarification of disease etiology and the development of comprehensive diagnostic or therapeutic tools, particularly for fastidious and difficult-to-cultivate bacteria. Using bovine
Boye, Mette, Jensen, Tim K., Klitgaard, Kirstine, Marcatili, Paolo, Nielsen, Martin W., Nielsen, Morten, Schafer Nielsen, Claus, Sicheritz Ponten, Thomas   +7 more
core   +7 more sources