Results 11 to 20 of about 1,539 (210)

Common light chain chickens produce human antibodies of high affinity and broad epitope coverage for the engineering of bispecifics

mAbs, 2021
Bispecific antibodies are an important and growing segment in antibody therapeutics, particularly in the immuno-oncology space. Manufacturing of a bispecific antibody with two different heavy chains is greatly simplified if the light chains can be the ...
Kathryn H. Ching, Kimberley Berg, Kevin Reynolds, Darlene Pedersen, Alba Macias, Yasmina N. Abdiche, William D. Harriman, Philip A. Leighton   +7 more
doaj   +1 more source

Quantitative and Qualitative Analysis of Humoral Immunity Reveals Continued and Personalized Evolution in Chronic Viral Infection

Cell Reports, 2020
Summary: Control of established chronic lymphocytic choriomeningitis virus (LCMV) infection requires the production of neutralizing antibodies, but it remains unknown how the ensemble of antibodies evolves during ongoing infection.
Nike Julia Kräutler, Alexander Yermanos, Alessandro Pedrioli, Suzanne P.M. Welten, Dominique Lorgé, Ute Greczmiel, Ilka Bartsch, Jörg Scheuermann, Jonathan D. Kiefer, Klaus Eyer, Ulrike Menzel, Victor Greiff, Dario Neri, Tanja Stadler, Sai T. Reddy, Annette Oxenius   +15 more
doaj   +1 more source

Assessment of antibody library diversity through next generation sequencing and technical error compensation. [PDF]

PLoS ONE, 2017
Antibody libraries are important resources to derive antibodies to be used for a wide range of applications, from structural and functional studies to intracellular protein interference studies to developing new diagnostics and therapeutics. Whatever the
Marco Fantini, Luca Pandolfini, Simonetta Lisi, Michele Chirichella, Ivan Arisi, Marco Terrigno, Martina Goracci, Federico Cremisi, Antonino Cattaneo   +8 more
doaj   +1 more source

A window into the human immune system: comprehensive characterization of the complexity of antibody complementary-determining regions in functional antibodies

mAbs, 2023
The human immune system uses antibodies to neutralize foreign antigens. They are composed of heavy and light chains, both with constant and variable regions. The variable region has six hypervariable loops, also known as complementary-determining regions
Oscar Mejias-Gomez, Andreas V. Madsen, Kerstin Skovgaard, Lasse E. Pedersen, J. Preben Morth, Timothy P. Jenkins, Peter Kristensen, Steffen Goletz   +7 more
doaj   +1 more source

A Novel Human scFv Library with Non-Combinatorial Synthetic CDR Diversity. [PDF]

PLoS ONE, 2015
The present work describes the construction and validation of a human scFv library with a novel design approach to synthetic complementarity determining region (CDR) diversification.
Xuelian Bai, Jihye Kim, Seungmin Kang, Wankyu Kim, Hyunbo Shim   +4 more
doaj   +1 more source

Epigenomic Modifications Mediating Antibody Maturation

Frontiers in Immunology, 2018
Epigenetic modifications, such as histone modifications, DNA methylation status, and non-coding RNAs (ncRNA), all contribute to antibody maturation during somatic hypermutation (SHM) and class-switch recombination (CSR).
Emily C. Sheppard, Rikke Brandstrup Morrish, Michael J. Dillon, Rebecca Leyland, Richard Chahwan   +4 more
doaj   +1 more source

Antibody Diversity in Cancer: Translational Implications and Beyond

Vaccines, 2022
Patients with cancer tend to develop antibodies to autologous proteins. This phenomenon has been observed across multiple cancer types, including bladder, lung, colon, prostate, and melanoma. These antibodies potentially arise due to induced inflammation
Raghuram Reddy, Joel Mintz, Roei Golan, Fakiha Firdaus, Roxana Ponce, Derek Van Booven, Aysswarya Manoharan, Isabelle Issa, Bonnie B. Blomberg, Himanshu Arora   +9 more
doaj   +1 more source

Honing-in antigen-specific cells during antibody discovery: a user-friendly process to mine a deeper repertoire

Communications Biology, 2022
The authors developed a methodology for bulk enrichment of antigen-specific B cells to improve the diversity of antibody sequences and increase the number of functional antigen-specific antibodies coming from different antibody generation platforms.
Ankit Mahendra, Aftabul Haque, Ponraj Prabakaran, Brian C. Mackness, Thomas P. Fuller, Xiaohua Liu, Sagar V. Kathuria, Yui-Hsi Wang, Nilesh Amatya, Xiaocong Yu, Joern Hopke, Dietmar Hoffmann, Eva Bric-Furlong, Ningning Zhang, Hyun-Suk Cho, Ruijun Zhang, Jose Sancho, Jacqueline Saleh, Sambasiva P. Rao, Maria Wendt, Partha S. Chowdhury   +20 more
doaj   +1 more source

Secondary Mechanisms of Affinity Maturation in the Human Antibody Repertoire

Frontiers in Immunology, 2013
V(D)J recombination and somatic hypermutation (SHM) are the primary mechanisms for diversification of the human antibody repertoire. These mechanisms allow for rapid humoral immune responses to a wide range of pathogenic challenges.
Bryan S. Briney, James E. Crowe
doaj   +1 more source

Systems Analysis Reveals High Genetic and Antigen-Driven Predetermination of Antibody Repertoires throughout B Cell Development

Cell Reports, 2017
Antibody repertoire diversity and plasticity is crucial for broad protective immunity. Repertoires change in size and diversity across multiple B cell developmental stages and in response to antigen exposure.
Victor Greiff, Ulrike Menzel, Enkelejda Miho, Cédric Weber, René Riedel, Skylar Cook, Atijeh Valai, Telma Lopes, Andreas Radbruch, Thomas H. Winkler, Sai T. Reddy   +10 more
doaj   +1 more source